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Interleukin 22 Receptor Antagonist Y51A Mouse Active Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS400114

Mono-pegylated (with 20 kDa PEG)recombinant mouse interleukin 22 Y51A mutant (numbering according to human IL-22 including signal peptide) is one polypeptide chain containing 147 amino, an additional Ala at N-terminus and one molecule of PEG 20 kDa at its… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
interleukin 22 receptor antagonist Y51A, Active Protein
Full Product Name
Mouse interleukin 22 receptor antagonist Y51A - pegylated
Product Synonym Names
Mouse pegylated interleukin 22 Y51A antagonist
Sequence
The sequence of the N-terminal amino acids could not be determined indicating that the PEG moiety is attached to the N-terminus .
Host
E Coli
Form/Format
White lyophilized powder.
Purity/Purification
Greater than 98.0% as determined by:
(a) Gel filtration analysis.
(b) Analysis by reducing and non-reducing SDS-PAGE gel.
Preparation and Storage
Lyophilized pegylated mouse IL22 although stable at room temperature for several days, should be stored desiccated below -20 degree C. Upon reconstitution at > 0.1 pegylated mIL22 mg/ml and up to 2 mg/ml and filter sterilization it can be stored at 4 degree C or even room temperature for several weeks making it suitable for long term infusion studies using osmotic pumps. At lower concentration addition of a carrier protein (0.1% HSA or BSA) is suggested. Please prevent freeze-thaw cycles.
Active Protein Note
Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.
Solubility
It is recommended to reconstitute the lyophilized pegylated mouse IL22 Y51A in sterile water or sterile 0.4% NaHCO3 adjusted to pH 8-9, not less than 100ug/ml, which can then be further diluted with other aqueous solutions.
Protein Content
Protein quantitation of mouse interleukin 22 Y51A mutant was carried out by UV spectroscopy at 280 nm using the absorbency value of 0.18 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the DNAman computer analysis program of protein sequences.
Dimers and Aggregates
The purified mouse pegylated IL22 consists of > 90% monomers as determined by gel-filtration chromatography.
Endotoxin
Less than 0.05 ng/ug (0.5 EU/ug) of recombinant pegylated IL-22 Y51A.
Formulation
The protein was lyophilized from a concentrated (1.0 mg/ml) solution with 0.003mM NaHCO3.
Biological Activity
murine IL-22 Y51A mutant is biologically active, capable of full inhibition of mouse IL-22 induced STAT3 phosphorylation in HepG cells. Its inhibitory acrtivity in vitro is ~ 10-20% compared to the non-pegylated mIL22 (Y51A) mutant.

Product Categories/Family for interleukin 22 receptor antagonist Y51A active protein

Hormones; Cytokines and Growth Factors

Other Notes

Small volumes of interleukin 22 receptor antagonist Y51A active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for interleukin 22 receptor antagonist Y51A purchase

MBS400114 is an active protein targeting interleukin 22 receptor antagonist Y51A. Also known as Mouse interleukin 22 receptor antagonist Y51A - pegylated. Host: E Coli.
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