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Resistin Antagonist Human Active Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS400115

Resistin, a product of the RSTN gene, is a peptide hormone belonging to the class of cysteine-rich secreted proteins (monomeric peptide contains 11 cysteine residues) referred to as the RELM family, and is also described as ADSF (Adipose Tissue-Specific… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
resistin antagonist, Active Protein
Full Product Name
Human resistin antagonist
Product Synonym Names
Human Resistin Antagonist
Sequence
The sequence of the first seven N-terminal amino acids was determined to be Ala-Ser-Se r-Lys-Thr- Leu-Ala.
Host
E Coli
Form/Format
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity/Purification
Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.
(c) Gel filtration chromatography under non denaturative conditions.
Preparation and Storage
Lyophilized resistin although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution it should be stored at 4 degree C between 2-7 days and for future use below -18 degree C. For long term storage (BUT NOT FOR in vivo EXPERIMENTS) it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Active Protein Note
Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.
Solubility
It is recommended to reconstitute the lyophilized resistin in sterile 18M-cm H2O not less than 100ug/ml, which can then be further diluted to other buffered aqueous solutions containing carrier proteins.
Protein Content
Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 1.26 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the computer analysis program of protein sequences (DNAman).
2. Analysis by RP-HPLC, using a standard solution of BSA as a Reference Standard.
Dimers and Aggregates
The protein is a covalent monomer and contains less than 2% aggregates.
Endotoxin
Less than 0.1 ng/ug (IEU/ug) of human resistin antagonist
Formulation
Recombinant resistin was lyophilized from a concentrated (1mg/ml) solution containg 0.03% NaHCO3.
Biological Activity
The biological activity was evidenced by resistin antagonist activity to inhibit resistin-induced Akt phosphorylation in two cell lines. It also reduced the weight (mainly the visceral fat) and normalized GTT and ITT inHFD-fed mice.

Product Categories/Family for resistin antagonist active protein

Hormones; Cytokines and Growth Factors

Other Notes

Small volumes of resistin antagonist active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for resistin antagonist purchase

MBS400115 is an active protein targeting resistin antagonist. Also known as Human resistin antagonist. Host: E Coli.
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