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Growth Hormone Antagonists (G119R) Rat Active Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS400126

Recombinant rat GH-22K G119R produced in E. coli is a single, non-glycosylated, polypeptide chain containing 191 amino acids and having a molecular mass of 22 kDa. It is mutated in single amino acid (G119R) according to similar bovine GH mutein described by… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Growth hormone antagonists (G119R), Active Protein
Full Product Name
Rat growth hormone antagonists (G119R)
Product Gene Name
G119R active protein
Product Synonym Names
Rat growth hormone antagonist (G119R)
Sequence
The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Phe-Pr o-Ala-Met.
Host
E Coli
Form/Format
Sterile-filtered white lyophilized (freeze-dried) powder.
The protein was lyophilized from a concentrated (1mg/ml) solution with 0.0045mM NaHCO3.
Purity/Purification
Greater than 98.0% as determined by: (a) Analysis by RP-HPLC.
(b) Gel filtration at non denaturing conditions using 25 nM Tris-HCl + 300 nM NaCl, pH 8.
(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.
Preparation and Storage
Lyophilized rGH G119R although stable at room temperature for at least two weeks, should be stored desiccated below -18 degree C. Upon reconstitution and filter sterilization oGH can be stored at 4 degree C, pH 9 for up to 4 weeks. For long term storage and more diluted solutions it is recommended to add a carrier protein (0.1% HSA or BSA). Addition of carrier protein should be avoided in in vivo experiments to prevent immunic response, Please prevent freeze-thaw cycles. 
Active Protein Note
Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.
Solubility
It is recommended to reconstitute the lyophilized rGH G119R in 0.4% NaHCO3 or water adjusted to pH 9, not less than 100ug/ml, which can then be further diluted to other aqueous solutions, preferably in a presence of a carrier protein such as BSA or similar.
Dimers and Aggregates
Less than 2% as determined by gel filtration chromatography
Biological Activity
rGH G119R acts as antagonist using an in vitro bioassay in PDF-P1 3B9 cells stably transfected with rabbit GH receptors. It is capable of forming a 1:1 complex with the recombinant ovine growth hormone receptor extracellular domain (ECD)and binds to this ECD with affinity similar the the wild type rGH.
Endotoxin
Less than 0.05 ng/ug (IEU/ug) of rat GH G119R.
Protein Content
Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 0.752 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the DNAman analysis program of protein sequences.
2. Analysis by RP-HPLC, using a calibrated solution of human GH-22K-pl as a Reference Standard.

Product Categories/Family for G119R active protein

Growth hormone and prolactin family

Other Notes

Small volumes of G119R active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for G119R purchase

MBS400126 is an active protein targeting G119R. Also known as Rat growth hormone antagonists (G119R). Host: E Coli.
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