The purified sheep anti-BrdU was assayed by competitive ELISA to demonstrate both specificity and sensitivity to BromodeoxyUridine. Purified antibody was incubated in solution with each of the following nucleotide inhibitors: Adenosine, Bromouridine, Bromodeoxyuridine, Thymidine, Deoxycytadine, and Uridine prior to incubation on plate coated with BrdU. The data below shows that antibody incubated with BrdU bound to the molecule in solution, and therefore shows lower signal at lower dilutions. As the sample is diluted, more of the antibody is available to bind the BrdU on the plate. The result is an increase in signal as titer increases.
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