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FAKD3 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS9604743

Function: Required for normal mitochondrial respiration (PubMed:20869947). Increases steady-state levels and half-lives of a subset of mature mitochondrial mRNAs MT-ND2, MT-ND3, MT-CYTB, MT-CO2, and MT-ATP8/6. Promotes MT-CO1 mRNA translation and increases… More details

$325 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
FAKD3, Polyclonal Antibody
Full Product Name
FAKD3 Antibody
Product Gene Name
anti-FAKD3 antibody
Product Synonym Gene Name
FASTKD3
Product Synonym Names
FAKD3_HUMAN; FAST kinase domain containing protein 3; FAST kinase domain-containing protein 3; FAST kinase domains 3; Fastkd3; FLJ23274; MGC142123; MGC5297
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Concentration
1mg/ml (lot specific)
Specificity
FAKD3 antibody detects endogenous levels of total FAKD3
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Antibody/Peptide Pairs
FAKD3 peptide (MBS9623460) is used for blocking the activity of FAKD3 antibody (MBS9604743)
Molecular Weight
Observed: 75 kD
Predicted: 76 kDa
3D Structure
ModBase 3D Structure for Q14CZ7
Supply Chain Verification
Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available, please inquire.
Immunogen
A synthesized peptide
Subcellular Location
Mitochondrion
Tissue Specificity
Expression detected in spleen, thymus, testis, ovary, colon, heart, smooth muscle, kidney, brain, lung, liver and white adipose tissue with highest expression in liver and thyroid.
Predicted Cross Reactivity
Pig, Bovine, Sheep
Similarity
Pig (83%), Bovine (82%), Sheep (82%)
Conjugation
Unconjugated
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-FAKD3 antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes for anti-FAKD3 antibody
WB: 1:500-1:1000
IF/ICC: 1:100-1:500

Western Blot (WB) of anti-FAKD3 antibody

Western blot analysis of extracts from HepG2 cells, using FAKD3 antibody.The lane on the left is treated with the antigen-specific peptide.
Rabbit Anti-FAKD3 Polyclonal Antibody – Cat# MBS9604743

Immunofluorescene (IF) of anti-FAKD3 antibody

MBS9604743 staining HepG2 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
Rabbit Anti-FAKD3 Polyclonal Antibody, Immunofluorescene (IF) – Cat# MBS9604743

Other Notes

Small volumes of anti-FAKD3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for FAKD3 purchase

MBS9604743 is an antibody targeting FAKD3. Also known as FAKD3 Antibody. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for FAKD3. It may not necessarily be applicable to this product.

NCBI GI #
40068497
NCBI GeneID
79072
NCBI Accession #
NP_076996.2
UniProt Primary Accession #
Q14CZ7
UniProt Secondary Accession #
Q9BVD3
UniProt Related Accession #
Q14CZ7
GenBank Nucleotide #
NM_024091.3
NCBI Official Full Name
FAST kinase domain-containing protein 3, mitochondrial
NCBI Official Synonym Full Names
FAST kinase domains 3
NCBI Official Symbol
FASTKD3
UniProt Protein Name
FAST kinase domain-containing protein 3, mitochondrial
UniProt Gene Name
FASTKD3
NCBI Protein Information
FAST kinase domain-containing protein 3, mitochondrial

NCBI Summary for FAKD3

This gene encodes a member of a small family of Fas-activated serine/threonine kinase domain (FASTKD) containing proteins that share an amino terminal mitochondrial targeting domain and multiple carboxy terminal FAST domains as well as a putative RNA-binding RAP domain. The members of this family are ubiquitously expressed and are generally most abundant in mitochondria-enriched tissues such as heart, skeletal muscle and brown-adipose tissue. Some members of this protein family may play a role in apoptosis. The protein encoded by this gene interacts with components of the mitochondrial respiratory and translation networks. A pseudogene of this gene is also present on chromosome 5. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2013]
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UniProt Comments for FAKD3

Required for normal mitochondrial respiration (PubMed:20869947). Increases steady-state levels and half-lives of a subset of mature mitochondrial mRNAs MT-ND2, MT-ND3, MT-CYTB, MT-CO2, and MT-ATP8/6. Promotes MT-CO1 mRNA translation and increases mitochondrial complex IV assembly and activity (PubMed:27789713).
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Research Articles on FAKD3

1. Despite the fact that proteins of the FASTK family FASTKD1-5 share the same domains, they exhibit various-sometimes opposing-functions in almost all steps of mitochondrial RNA metabolism.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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