Mouse Anti-RHO Monoclonal Antibody – Cat# MBS415058

Rhodopsin (RHO) Antibody – Mouse Monoclonal

Name: RHO Antibody; Monoclonal; Mouse; MyBioSource
Brand: MyBioSource
SKU: MBS415058
Price: 445 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS415058

MBS415058 is an antibody targeting RHO. Also known as Rhodopsin Antibody. Reactive in Human, bovine, mouse, pig and rat. Host: Mouse. More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

Rhodopsin, Monoclonal Antibody

Full Product Name

Rhodopsin Antibody

Product Gene Name

anti-RHO antibody

Product Synonym Names

Rhodopsin

Clonality

Monoclonal

Isotype

IgG1

Host

Mouse

Species Reactivity

Human, bovine, mouse, pig and rat

Form/Format

Liquid

Purity/Purification

Affinity purified

Concentration

100ug/100ul (lot specific)

Preparation and Storage

Store at -20 degree C/1 year

Molecular Weight

38,893 Da

Chromosome Location

Chromosome: 3; NC_000003.11 (129247482..129254187). Location: 3q21-q24

OMIM

180380

3D Structure

ModBase 3D Structure for P08100

Immunogen

Rhodopsin is the protein in the mammalian retina responsible for the light sensitivity of rod cells, which are responsible for vision in low light levels. Somewhat surprisingly, the rhodopsin protein turned out to be a typical member of the seven transmembrane G protein-coupled receptor (GPCR) superfamily. Whereas other GPCRs initiate signaling on binding a specific ligand, rhodopsin exists with a ligand already bound, specifically the vitamin A related substance retinal. Retinal can exist in two isomeric forms, 11-cis and 11-trans retinal. In the dark rhodopsin is associated with 11-cis retinal, but photons cause the 11-cis form to flip to the 11-trans form, and this causes an alteration in the structure of the rhodopsin making it catalytically active. Activated rhodopsin in turn activates the GTP binding protein G protein transducin by favoring the loss of GDP and the addition of GTP. Transducin is a typical member of the family of heterotrimeric G proteins, and consists of an Î± and a betagamma subunit. The Î± subunit is responsible for the GTP binding and the GTP bound form activates a phosphodiesterase (PDE) enzyme which hydrolyses cyclic GMP. This in turn increases the membrane potential of the rod cell and reduces the rate of synaptic signaling. So light stimulation actually results in a reduced rate of photoreceptor synaptic release. This information is transmitted through neurons of the retina to the visual centers of the brain (see review 1, 2). Rhodopsin activity is shut off by phosphorylation under the influence of rhodopsin kinase, the activity of which results in binding of visual arrestin (a.k.a. arrestin-1 and S-antigen), which prevents rhodopsin from interacting with and activating more transducin molecules (3, 4). This basic signaling paradigm proved to be a prototype for understanding how other GPCRs function, as proteins similar to transducin, arrestin and rhodopsin kinase are found in these pathways. MCA-A531 was generated against whole purified bovine rhodopsin and shows convincing staining for rhodopsin both on western blots and on sections of retina. The HGNC name for this protein is RHO.

Storage Buffer

PBS, pH 7.4 with 0.02% sodium azide.

Applications Tested/Suitable for anti-RHO antibody

Immunofluorescence (IF), Western Blot (WB)

Application Notes for anti-RHO antibody

Try at dilutions of ~1:1,000 for immunofluorescence. For western blots try at 1:5,000.

Product References and Citations for anti-RHO antibody

1. Molday RS. Photoreceptor membrane proteins, phototransduction, and retinal degenerative disease. The Frienwald lecture. Invest Ophthalmol Vis Sci. 39:2491-513 (1998).2. Yau,KW. Phototransduction Mechanism in Retinal Rods and Cones. The Frienwald lecture. Invest Ophthalmol Vis Sci. 35:9-32 (1994).3. Wilden U, Hall SW, Kohn H. Phosphodiesterase activation by photoexcited rhodopsin is quenched when rhodopsin is phosphorylated and binds the intrinsic 48-kDa protein of rod outer segments. Proc Natl Acad Sci USA 83:1174-8 (1986).4. Smith WC, Mc Dowell JH, Dugger DR, Miller R, Arendt A, Popp MP, Hargrave PA. Identification of regions of arrestin that bind to rhodopsin. Biochemistry Mar 38:2752-61 (1999).

Other Notes

Small volumes of anti-RHO antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for RHO. It may not necessarily be applicable to this product.

NCBI GI #

4506527

NCBI GeneID

6010

NCBI Accession #

NP_000530.1

UniProt Primary Accession #

P08100

UniProt Secondary Accession #

Q16414; Q2M249

UniProt Related Accession #

P08100

GenBank Nucleotide #

NM_000539.3

NCBI Official Full Name

rhodopsin

NCBI Official Synonym Full Names

rhodopsin

NCBI Official Symbol

RHO

NCBI Official Synonym Symbols

RP4; OPN2; CSNBAD1

UniProt Protein Name

Rhodopsin

UniProt Synonym Protein Names

Opsin-2

Protein Family

Protein rhomboid

UniProt Gene Name

RHO

UniProt Synonym Gene Names

OPN2

UniProt Entry Name

OPSD_HUMAN

NCBI Protein Information

rhodopsin; opsin-2; opsin 2, rod pigment

NCBI Summary for RHO

Retinitis pigmentosa is an inherited progressive disease which is a major cause of blindness in western communities. It can be inherited as an autosomal dominant, autosomal recessive, or X-linked recessive disorder. In the autosomal dominant form,which comprises about 25% of total cases, approximately 30% of families have mutations in the gene encoding the rod photoreceptor-specific protein rhodopsin. This is the transmembrane protein which, when photoexcited, initiates the visual transduction cascade. Defects in this gene are also one of the causes of congenital stationary night blindness. [provided by RefSeq, Jul 2008]

UniProt Comments for RHO

Function: Photoreceptor required for image-forming vision at low light intensity. Required for photoreceptor cell viability after birth. Light-induced isomerization of 11-cis to all-trans retinal triggers a conformational change leading to G-protein activation and release of all-trans retinal.

Subunit structure: Homodimer

By similarity.

Subcellular location: Membrane; Multi-pass membrane protein. Note: Synthesized in the inner segment (IS) of rod photoreceptor cells before vectorial transport to the rod outer segment (OS) photosensory cilia. Ref.33

Tissue specificity: Rod shaped photoreceptor cells which mediates vision in dim light.

Post-translational modification: Phosphorylated on some or all of the serine and threonine residues present in the C-terminal region.Contains one covalently linked retinal chromophore

By similarity.

Involvement in disease: Retinitis pigmentosa 4 (RP4) [MIM:613731]: A retinal dystrophy belonging to the group of pigmentary retinopathies. Retinitis pigmentosa is characterized by retinal pigment deposits visible on fundus examination and primary loss of rod photoreceptor cells followed by secondary loss of cone photoreceptors. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.7 Ref.8 Ref.9 Ref.10 Ref.11 Ref.12 Ref.13 Ref.14 Ref.15 Ref.16 Ref.17 Ref.18 Ref.20 Ref.21 Ref.22 Ref.23 Ref.24 Ref.25 Ref.26 Ref.28 Ref.30 Ref.31 Ref.33 Ref.34 Ref.35Night blindness, congenital stationary, autosomal dominant 1 (CSNBAD1) [MIM:610445]: A non-progressive retinal disorder characterized by impaired night vision, often associated with nystagmus and myopia.Note: The disease is caused by mutations affecting the gene represented in this entry.

Sequence similarities: Belongs to the G-protein coupled receptor 1 family. Opsin subfamily.

Biophysicochemical propertiesAbsorption:Abs(max)=495 nm

Research Articles on RHO

1. The S186W mutant thermally destabilizes rhodopsin by disrupting a hydrogen bond network at the receptor's active site.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-RHO antibody

Reference Product	PubMed Publications
NRL antibody	>28 publications with RHO and NRL
GRK1 antibody	>14 publications with RHO and GRK1
GNAT1 antibody	>10 publications with RHO and GNAT1
ADRBK1 antibody	>9 publications with RHO and ADRBK1
SAG antibody	>6 publications with RHO and SAG
RCVRN antibody	>3 publications with RHO and RCVRN
GNGT1 antibody	>1 publications with RHO and GNGT1

Pathways associated with anti-RHO antibody

Products by Pathway	Pathway Diagram
Activation Of The Phototransduction Cascade Pathway antibodies	Activation Of The Phototransduction Cascade Pathway Diagram
Class A/1 (Rhodopsin-like Receptors) Pathway antibodies	Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
Disease Pathway antibodies	Disease Pathway Diagram
Diseases Associated With Visual Transduction Pathway antibodies	Diseases Associated With Visual Transduction Pathway Diagram
G Alpha (i) Signalling Events Pathway antibodies	G Alpha (i) Signalling Events Pathway Diagram
GPCR Downstream Signaling Pathway antibodies	GPCR Downstream Signaling Pathway Diagram
GPCR Ligand Binding Pathway antibodies	GPCR Ligand Binding Pathway Diagram
GPCRs, Class A Rhodopsin-like Pathway antibodies	GPCRs, Class A Rhodopsin-like Pathway Diagram
Inactivation, Recovery And Regulation Of The Phototransduction Cascade Pathway antibodies	Inactivation, Recovery And Regulation Of The Phototransduction Cascade Pathway Diagram
Integrated Breast Cancer Pathway antibodies	Integrated Breast Cancer Pathway Diagram

Diseases associated with anti-RHO antibody

Disease Name	Pubmed Publications
Inflammation Antibodies	>1073 publications with RHO and Inflammation
Heart Diseases Antibodies	>869 publications with RHO and Heart Diseases
Musculoskeletal Diseases Antibodies	>736 publications with RHO and Musculoskeletal Diseases
Necrosis Antibodies	>563 publications with RHO and Necrosis
Neoplasm Metastasis Antibodies	>545 publications with RHO and Neoplasm Metastasis
Liver Diseases Antibodies	>494 publications with RHO and Liver Diseases
Fibrosis Antibodies	>453 publications with RHO and Fibrosis
Kidney Diseases Antibodies	>435 publications with RHO and Kidney Diseases
Drug Toxicity Antibodies	>434 publications with RHO and Drug Toxicity
Hypertrophy Antibodies	>284 publications with RHO and Hypertrophy

Organs/Tissues associated with anti-RHO antibody

Organ/Tissue Name	Pubmed Publications
Muscle Antibodies	>2848 publications with RHO and Muscle
Brain Antibodies	>2212 publications with RHO and Brain
Eye Antibodies	>1020 publications with RHO and Eye
Uncharacterized Tissue Antibodies	>7 publications with RHO and Uncharacterized Tissue