Neurofilament Nf-H Antibody – Mouse Monoclonal

Monoclonal

IgG2b

Mouse

Liquid

Affinity purified

100ug/100ul (lot specific)

The antibody recognizes NF-H strongly in all mammals tested to date and also in chicken.

This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.

Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. NF-H is the neurofilament high or heavy molecular weight polypeptide and runs on SDS-PAGE gels at 200-220 kDa, with some variability across species boundaries. Antibodies to NF-H are useful for identifying neuronal cells and their processes in tissue sections and in tissue culture. NF-H antibodies can also be useful in the neurofilament accumulations seen in many neurological diseases, such as Amyotrophic Lateral Sclerosis (also known as Lou Gehrig's disease) and Alzheimer's disease. MCA-9B12 is one of numerous antibodies which reacts preferentially with the axonal phosphorylated forms of NF-H. Interestingly these phosphorylated forms of NF-H are normally restricted to axons, while less phosphorylated forms are found in dendrites. However in numerous damage and disease states, phosphorylated NF-H can be detected with MCA-9B12 in dendritic and perikaryal neurofilaments.

PBS, pH 7.4 with 0.02% sodium azide.