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HA Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS415357

Hemagglutinin is an antigenic glycoprotein found on the surface of the influenza viruses, as well as many other bacteria and viruses. It is responsible for binding the virus to the cell that is being infected. The name hemagglutinin comes from the protein's… More details

$400 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
HA, Monoclonal Antibody
Full Product Name
Anti-HA Antibody
Product Gene Name
Clonality
Monoclonal
Isotype
IgG3
Clone Number
HA.C5
Host
Mouse
Form/Format
Liquid
Concentration
100ug/100ul (lot specific)
Specificity
HA-tag monoclonal antibody. Recognizes HA-tagged tagged proteins overpressed in cells, including both amino- or carboxy-termini of targeted proteins in transfected mammalian cells.
Preparation and Storage
This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
Storage Buffer
PBS, pH 7.4 with 0.05% sodium azide.
Immunogen
A synthetic peptide from influenza hemagglutinin epitope (YPYDVPDYA) coupled to KLH.
Applications Tested/Suitable for anti-HA antibody
Western Blot (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemsitry (IHC), Immunocytochemistry (ICC)
Application Notes for anti-HA antibody
ICC/IHC/IF/IP 1:200 dilution
WB 1:1000 dilution.

Testing Data of anti-HA antibody

Mouse Anti-HA Monoclonal Antibody – Cat# MBS415357

Product References and Citations for anti-HA antibody

Harvey, MC et al. "Distinct effects of the mesenchymal dysplasia gene variant of murine Patched-1 protein on canonical and non-canonical Hedgehog signaling pathways." J. Biol. Chem. 289:10939-49 (2014). PubMed: 24570001.
Ji, YJ et al. "EphrinB2 affects apical constriction in Xenopus embryos and is regulated by ADAM10 and flotillin-1." Nat Commun 5:3516 (2014). PubMed: 24662724.
Dup, A et al. "An Alba-domain protein contributes to the stage-regulated stability of amastin transcripts in Leishmania." Mol. Microbiol. 91:548-61 (2014). PubMed: 24620725.
Son, J et al. "Pick1 modulates ephrinB1-induced junctional disassembly through an association with ephrinB3." Biochem. Biophys. Res. Commun. 1:659-667 (2014). PubMed: 24937449.
Whittington,, N. et al. "Sox21 regulates the progression of neuronal differentiation in a dose-dependent manner." Developmental Biology 397 (2):237-247 (2014). PubMed: 25448693.
Irie, T et al. "Significance of the YLDL motif in the M protein and Alix/AIP1 for Sendai virus budding in the context of virus infection." Virology 405 (2):334-341 (2013). DOI: 10.1016/j.virol.2010.06.031. PubMed: 20605035. Application: Western Blot.
Bronnimann, MP et al. "A transmembrane domain and GxxxG motifs within L2 are essential for papillomavirus infection." J Virol 87 (1):464-473 (2013). DOI: 10.1128/JVI.01539-12. Application: Western Blot.
Daniel, CJ et al. "Detection of c-Myc protein-protein interactions and phosphorylation status by immunoprecipitation." Methods Mol Biol 1012:65-76 (2013). DOI: 10.1007/978-1-62703-429-6_5. PubMed: 24006058. Application: Immunoprecipitation.
Hwang, YS et al. "The Smurf ubiquitin ligases regulate tissue separation via antagonistic interactions with ephrinB1." Genes Dev. 27:491-503 (2013). PubMed: 23475958.
Lee, JY et al. "Cell growth defect factor1, chaperone-like protein of POR1 plays a role in stabilization of light-dependent protochlorophyllide oxidoreductase in Nicotiana benthamiana and Arabidopsis." Plant Cell 25:3944-60 (2013). PubMed: 24151298.
Su, B et al. "Adhesion-mediated cytoskeletal remodeling is controlled by the direct scaffolding of Src from FAK complexes to lipid rafts by SSeCKS, AKAP12." Oncogene 32:2016-26 (2013). PubMed: 22710722.
Gong, R et al. "Bispecific engineered antibody domains (nanoantibodies) that interact noncompetitively with an HIV-1 neutralizing epitope and FcRn." PLoS ONE 7:e42288 (2012). PubMed: 22879932.
Neilson, KM et al. "Specific domains of FoxD4, 5 activate and repress neural transcription factor genes to control the progression of immature neural ectoderm to differentiating neural plate." Dev. Biol. 365:363-75 (2012). PubMed: 22425621.
Irie, T et al. "Inhibition of interferon regulatory factor 3 activation by paramyxovirus V protein." J. Virol. 86:7136-45 (2012). PubMed: 22532687.
Ma, Y et al. "Inhibition of TANK binding kinase 1 by herpes simplex virus 1 facilitates productive infection." J. Virol. 86:2188-96 (2012). PubMed: 22171259. Application: Immunoblot.
Liu, X et al. "Molecular and structural characterization of the SH3 domain of AHI-1 in regulation of cellular resistance of BCR-ABL(+) chronic myeloid leukemia cells to tyrosine kinase inhibitors." Proteomics 12:2094-106 (2012). PubMed: 22623184.
Parker, JG et al. " The Contribution of NMDA Receptor Signaling in the Corticobasal Ganglia Reward Network to Appetitive Pavlovian Learning." J Neurosci 31 (31):11362-11369 (2011). DOI: 10.1523/JNEUROSCI.2411-11.2011. Application: Immunostaining.
Leung, GP et al. "Rtt107 is required for recruitment of the SMC5/6 complex to DNA double strand breaks." J Biol Chem 286 (29):26250-7 (2011). PubMed: 21642432. Application: Immunoprecipitation.
Jin, H et al. "A Herpesvirus Virulence Factor Inhibits Dendritic Cell Maturation through Protein Phosphatase 1 and IkappaB Kinas." J Virol 85 (7):3397-3407 (2011). DOI: 10.1128/JVI.02373-10. Application: Western Blot.
Cho, HJ et al. "EphrinB1 Interacts with CNK1 and Promotes Cell Migration through JNK Activation." J. Biol. Chem. : (2014). PubMed: 24825906.
Escamilla-Powers, JR et al. "The tumor suppressor protein HBP1 is a novel c-myc-binding protein that negatively regulates c-myc transcriptional activity.." J Biol Chem 285 (7):4847-4858 (2010). DOI: 10.1074/jbc.M109.074856. Application: Immunoprecipitation.
Armistead, J et al. "Mutation of a Gene Essential for Ribosome Biogenesis, EMG1, Causes Bowen-Conradi Syndrome." Am J Hum Genet 84 (6):728-739 (2009). DOI: 10.1016/j.ajhg.2009.04.017. Application: Immunoblot.
Charbonneau, Me et al. "Autoprocessing of the Escherichia coli AIDA-I autotransporter: a new mechanism involving acidic residues in the junction region." J Biol Chem 284 (25):17340-17351 (2009). DOI: 10.1074/jbc.M109.010108. Application: Immunodection.
Verpooten, D et al. "Control of TANK-binding kinase 1-mediated signlaing by the y134. 5 protein of herpes simplex virus 1.." J Biol Chem 284 (2):1097-1105 (2009). DOI: 10.1074/jbc.M805905200. Application: Immunoblot.
Hayashi, A et al. "Pyripyropenes, fungal sesquiterpenes conjugated with alpha-pyrone and pyridine moieties, exhibits anti-angiogenic activity against human umbilical vein endothelial cells." Biol Pharm Bull 32 (7):1261-1205 (2009). PubMed: 19571395. Application: Western Blot.
Burston, HE et al. "Regulators of yeast endocytosis identified by systematic quantitative analysis." J. Cell Biol. 185:1097-110 (2009). PubMed: 19506040.
Lee, HK et al. "Drought stress-induced Rma1H1, a RING membrane-anchor E3 ubiquitin ligase homolog, regulates aquaporin levels via ubiquitination in transgenic Arabidopsis plants." Plant Cell 21:622-41 (2009). PubMed: 19234086.
Barr, DJ et al. "Clathrin-independent endocytosis of ErbB2 in geldanamycin-treated human breast cancer cells." J Cell Sci 121:3155-66 (2008). PubMed: 18765569.
Lee, HS et al. "EphrinB1 controls cell-cell junctions through the Par polarity complex." Nat. Cell Biol. 10:979-86 (2008). PubMed: 18604196.
Li, P et al. "Multiple roles of the candidate oncogene ZNF217 in ovarian epithelial neoplastic progression." Int J Cancer 120 (9):1863-1873 (2007). PubMed: 17266044. Application: Western Blot.
Green, JL et al. "The C. elegans ROR receptor tyrosine kinase, CAM-1, non-autonomously inhibits the Wnt pathway." Development 124 (22):4053-4062 (2007). PubMed: 17942487. Application: Immunoblotting.
Prieto-Echage, V et al. "Regulation of Ack1 localization and activity by the amino-terminal SAM domain." BMC Biochem. 11:42 (2010). PubMed: 20979614.

Other Notes

Small volumes of anti-HA antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for HA purchase

MBS415357 is an antibody targeting HA. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for HA. It may not necessarily be applicable to this product.

NCBI GI #
NCBI Accession #
NCBI Official Full Name
HA tag, partial
Protein Family

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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