Rabbit Anti-EARS2 Polyclonal Antibody – Cat# MBS415446

EARS2 Antibody – Rabbit Polyclonal

Name: EARS2 Antibody; Polyclonal; Rabbit; Human/Mouse/Rat-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS415446
Price: 335 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS415446

Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. This gene belongs to the class-I aminoacyl-tRNA synthetase family and catalyzes the attachment of glutamate to tRNA(Glu). Alternative splicing results in… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

EARS2, Polyclonal Antibody

Full Product Name

Anti-EARS2 Antibody

Product Gene Name

anti-EARS2 antibody

Clonality

Polyclonal

Host

Rabbit

Species Reactivity

Human. Predicted: Mouse, Rat.

Form/Format

Liquid

Purity/Purification

Affinity purified

Concentration

100ug/100ul (lot specific)

Preparation and Storage

This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.

Molecular Weight

59,989 Da

OMIM

612799

Storage Buffer

PBS, pH 7.4 with 0.02% Sodium Azide

Immunogen

Rabbit polyclonal EARS2 (1) antibody was raised against a recombinate human EARS2 protein 22-253 aa (BC040013).

Applications Tested/Suitable for anti-EARS2 antibody

ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)

Application Notes for anti-EARS2 antibody

ELISA titer: 1:20,000-1:80,000
Western blot analysis: 1:1,000-1:2,000
Immunohistochemistry: 1:100-1:500
Optimal dilutions/concentrations should be determined by the end user.

Product References and Citations for anti-EARS2 antibody

(1) Hendrickson,S.L., Lautenberger,J.A., Chinn,L.W. et al. Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression. PLoS ONE 5 (9), E12862 (2010).

Other Notes

Small volumes of anti-EARS2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for EARS2 purchase

MBS415446 is an antibody targeting EARS2. Reactive in Human. Predicted: Mouse, Rat.. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for EARS2. It may not necessarily be applicable to this product.

NCBI GI #

25058989

NCBI GeneID

124454

NCBI Accession #

AAH40013.1

UniProt Secondary Accession #

Q86YH3; Q8TF31; B3KTT2; D3DWF1

UniProt Related Accession #

Q5JPH6

NCBI Official Full Name

EARS2 protein

NCBI Official Synonym Full Names

glutamyl-tRNA synthetase 2, mitochondrial

NCBI Official Symbol

EARS2

NCBI Official Synonym Symbols

MSE1; COXPD12

UniProt Protein Name

Probable glutamate--tRNA ligase, mitochondrial

UniProt Synonym Protein Names

Glutamyl-tRNA synthetase; GluRS

Protein Family

Probable glutamate--tRNA ligase

UniProt Gene Name

EARS2

UniProt Entry Name

SYEM_HUMAN

NCBI Protein Information

probable glutamate--tRNA ligase, mitochondrial; gluRS; glutamate tRNA ligase 2, mitochondrial; probable glutamyl-tRNA synthetase, mitochondrial

NCBI Summary for EARS2

This gene encodes a member of the class I family of aminoacyl-tRNA synthetases. These enzymes play a critical role in protein biosynthesis by charging tRNAs with their cognate amino acids. This protein is encoded by the nuclear genome but is likely to be imported to the mitochondrion where it is thought to catalyze the ligation of glutamate to tRNA molecules. Mutations in this gene have been associated with combined oxidative phosphorylation deficiency 12 (COXPD12). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2015]

UniProt Comments for EARS2

EARS2: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu). Belongs to the class-I aminoacyl-tRNA synthetase family.

Protein type: Cofactor and Vitamin Metabolism - porphyrin and chlorophyll; Ligase; Mitochondrial; EC 6.1.1.17; Translation

Chromosomal Location of Human Ortholog: 16p12.2

Cellular Component: mitochondrion; mitochondrial matrix

Molecular Function: glutamate-tRNA(Gln) ligase activity; glutamate-tRNA ligase activity; ATP binding; tRNA binding

Biological Process: tRNA aminoacylation for protein translation; glutamyl-tRNA aminoacylation; gene expression

Disease: Combined Oxidative Phosphorylation Deficiency 12

Research Articles on EARS2

1. This study presented that a new neurological disease, early-onset leukoencephalopathy with thalamus and brainstem involvement and high lactate, defined by a peculiar biphasic clinical course and caused by mutations in a single gene, EARS2.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-EARS2 antibody

Products by Pathway	Pathway Diagram
Aminoacyl-tRNA Biosynthesis Pathway antibodies	Aminoacyl-tRNA Biosynthesis Pathway Diagram
Aminoacyl-tRNA Biosynthesis Pathway antibodies	Aminoacyl-tRNA Biosynthesis Pathway Diagram
Aminoacyl-tRNA Biosynthesis, Eukaryotes Pathway antibodies	Aminoacyl-tRNA Biosynthesis, Eukaryotes Pathway Diagram
Aminoacyl-tRNA Biosynthesis, Eukaryotes Pathway antibodies	Aminoacyl-tRNA Biosynthesis, Eukaryotes Pathway Diagram
Gene Expression Pathway antibodies	Gene Expression Pathway Diagram
Metabolic Pathways antibodies	Metabolic Pathways Diagram
Mitochondrial TRNA Aminoacylation Pathway antibodies	Mitochondrial TRNA Aminoacylation Pathway Diagram
Porphyrin And Chlorophyll Metabolism Pathway antibodies	Porphyrin And Chlorophyll Metabolism Pathway Diagram
Porphyrin And Chlorophyll Metabolism Pathway antibodies	Porphyrin And Chlorophyll Metabolism Pathway Diagram
TRNA Aminoacylation Pathway antibodies	TRNA Aminoacylation Pathway Diagram

Diseases associated with anti-EARS2 antibody

Disease Name	Pubmed Publications
Brain Diseases Antibodies	>2 publications with EARS2 and Brain Diseases
Nervous System Diseases Antibodies	>2 publications with EARS2 and Nervous System Diseases

Organs/Tissues associated with anti-EARS2 antibody

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>2 publications with EARS2 and Brain
Skin Antibodies	>1 publications with EARS2 and Skin