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N-Acetylglucosaminyltransferase V (MGAT5) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS415850

MBS415850 is an antibody targeting MGAT5. Also known as Anti-N-acetylglucosaminyltransferase V Antibody. Reactive in Human, Mouse, Rat. Not yet tested in other species.. Host: Rabbit. More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
N-acetylglucosaminyltransferase V (MGAT5), Polyclonal Antibody
Full Product Name
Anti-N-acetylglucosaminyltransferase V Antibody
Product Gene Name
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat. Not yet tested in other species.
Form/Format
Liquid
Purity/Purification
Affinity purified
Concentration
100ug/100ul (lot specific)
Preparation and Storage
This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
Molecular Weight
84,543 Da
OMIM
601774
Storage Buffer
PBS, pH 7.4 with 0.02% Sodium Azide
Immunogen
Rabbit polyclonal N-acetylglucosaminyl -transferase V antibody was raised against a peptide at the C terminal residues of human N-acetylglucosaminyl -transferase V protein.
Applications Tested/Suitable for anti-MGAT5 antibody
ELISA (EIA), Immunohistochemistry (IHC)
Application Notes for anti-MGAT5 antibody
Peptide ELISA titer: 1:40,000-1:80,000
Immunohistochemistry: 1:50-200
Optimal dilutions/concentrations should be determined by the end user.

Product References and Citations for anti-MGAT5 antibody

(1) Saito,H., Nishikawa,A., Gu,J., et al.cDNA cloning and chromosomal mapping of human N-acetylglucosaminyltransferase V+.Biochem. Biophys. Res. Commun. 198 (1), 318-327 (1994)

Other Notes

Small volumes of anti-MGAT5 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for MGAT5. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
N-acetylglucosaminyltransferase V
NCBI Official Synonym Full Names
mannosyl (alpha-1,6-)-glycoprotein beta-1,6-N-acetyl-glucosaminyltransferase
NCBI Official Symbol
MGAT5
NCBI Official Synonym Symbols
GNT-V; GNT-VA
UniProt Protein Name
Alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A
UniProt Synonym Protein Names
Alpha-mannoside beta-1,6-N-acetylglucosaminyltransferase; GlcNAc-T V; GNT-V; Mannoside acetylglucosaminyltransferase 5; N-acetylglucosaminyl-transferase V
UniProt Gene Name
MGAT5
UniProt Entry Name
MGT5A_HUMAN
NCBI Protein Information
alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A; N-acetylglucosaminyl-transferase V; alpha-mannoside beta-1,6-N-acetylglucosaminyltransferase; glcNAc-T V; mannoside acetylglucosaminyltransferase 5

NCBI Summary for MGAT5

The protein encoded by this gene belongs to the glycosyltransferase family. It catalyzes the addition of beta-1,6-N-acetylglucosamine to the alpha-linked mannose of biantennary N-linked oligosaccharides present on the newly synthesized glycoproteins. It is one of the most important enzymes involved in the regulation of the biosynthesis of glycoprotein oligosaccharides. Alterations of the oligosaccharides on cell surface glycoproteins cause significant changes in the adhesive or migratory behavior of a cell. Increase in the activity of this enzyme has been correlated with the progression of invasive malignancies. [provided by RefSeq, Oct 2011]
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UniProt Comments for MGAT5

MGAT5: Catalyzes the addition of N-acetylglucosamine in beta 1- 6 linkage to the alpha-linked mannose of biantennary N-linked oligosaccharides. It is one of the most important enzymes involved in the regulation of the biosynthesis of glycoprotein oligosaccharides. Belongs to the glycosyltransferase 18 family.

Protein type: Membrane protein, integral; Transferase; Glycan Metabolism - N-glycan biosynthesis; EC 2.4.1.155

Chromosomal Location of Human Ortholog: 2q21.3

Cellular Component: Golgi membrane; integral to membrane

Molecular Function: alpha-1,6-mannosyl-glycoprotein 6-beta-N-acetylglucosaminyltransferase activity

Biological Process: cellular protein metabolic process; protein amino acid N-linked glycosylation via asparagine; post-translational protein modification
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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