At 37 degree C. The primary antibody was diluted 1/400 and incubated with the sample for 1 hour at 37 degree C. A Alexa Fluor 594 conjugated goat polyclonal to rabbit IgG (H+L), diluted 1/600 was used as secondary antibod
At 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibod
At 1/100 staining Human cervical cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary
Western blot analysis of Cox2 expression in A549 whole cell lysates,The lane on the left is treated with the antigen-specific peptide.
Western blot analysis of extracts from Mouse spleen, using Cox2 Antibody. The lane on the left was treated with blocking peptide.
Observed bands: 70 kDa.
E4BP4 mediates glucocorticoid-regulated adipogenesis through COX2 Yang
Yang a, b , Hongkui Wei a, b , Tongxing Song a, b , Anle Cai a, b , Yuanfei Zhou a, b , Jie Peng a, b , Siwen Jiang b, c , Jian Peng a, b, *
MBS9610298 staining A549 cells cells by ICC/IF. Cells were fixed with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 minutes at 37°C. The primary antibody was diluted 1/400 and incubated with the sample for 1 hour at 37°C. A Alexa Fluor® 594 conjugated goat polyclonal to rabbit IgG (H+L), diluted 1/600 was used as secondary antibody.
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