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tubulin Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS416876

MBS416876 is an antibody targeting tubulin. Host: Mouse. More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
Tubulin, Monoclonal Antibody
Full Product Name
Anti-Tubulin Antibody
Product Gene Name
Clonality
Monoclonal
Isotype
IgG
Clone Number
A10
Host
Mouse
Form/Format
Liquid
Concentration
100ug/100ul (lot specific)
Specificity
Recognize alpha-tubulin from Human, Mouse, Tetrahymena, dictyostelium, Saccharomyces.
Preparation and Storage
This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
Molecular Weight
46,955 Da
Storage Buffer
PBS, pH 7.4 with 0.05% sodium azide.
Immunogen
Recombinant alpha-tubulin protein from bacteria.
Applications Tested/Suitable for anti-tubulin antibody
Western Blot (WB), Immunofluorescence (IF)
Application Notes for anti-tubulin antibody
Western blot at dilution of 1:1000
Immunofluorescence staining at 1:100 dilutions
It is very useful as a loading control for quantitive western blotting. The antibody reconizes a 50kDa band in a Western Blot analysis.

Western Blot (WB) of anti-tubulin antibody

Mouse Anti-tubulin Monoclonal Antibody – Cat# MBS416876

Product References and Citations for anti-tubulin antibody

Park, IH et al. "Inhibition of hepatitis B virus replication by ligand-mediated activation of RNase L." Antiviral Res. 104:118-27 (2014). PubMed: 24509240.
Baek, KH et al. "Thrombospondin-1 mediates oncogenic Ras-induced senescence in premalignant lung tumors." J. Clin. Invest. 123:4375-89 (2013). PubMed: 24018559.
Bernasconi, R et al. "Autoadaptive ER-associated degradation defines a preemptive unfolded protein response pathway." Mol. Cell 52:783-93 (2013). PubMed: 24239290.
Reggiori, F et al. "Coronaviruses Hijack the LC3-I-positive EDEMosomes, ER-derived vesicles exporting short-lived ERAD regulators, for replication." Cell Host Microbe 7:500-8 (2010). PubMed: 20542253.

Other Notes

Small volumes of anti-tubulin antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for tubulin. It may not necessarily be applicable to this product.

NCBI GI #
NCBI Accession #
NCBI Official Full Name
tubulin, partial
UniProt Protein Name
Tubulin
UniProt Synonym Protein Names
Tubulin
Protein Family
UniProt Gene Name
tubulin
UniProt Entry Name
A0A090I464_9EUKA

UniProt Comments for tubulin

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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