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Beta-Tubulin (TUBB2B) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS200193

Tubulin is a major cytoskeleton component that has five distinct forms. Multiple beta Tubulin isoforms have been characterized and are expressed in mammalian tissues. Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an… More details

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Product Overview

Online Datasheet in PDF
Product Name
beta-Tubulin (TUBB2B), Monoclonal Antibody
Full Product Name
beta-Tubulin antibody
Product Gene Name
anti-TUBB2B antibody
Product Synonym Names
DKFZp566F223; FLJ98847; MGC8685; Tubulin beta-2B chain; bA506K6.1; TUBB2B; Tubulin beta 2b; Tubulin beta-2B
Clonality
Monoclonal
Isotype
IgG2b,k
Clone Number
AT5B2
Host
Mouse
Species Reactivity
Human, Mouse
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Purity/Purification
By protein-G affinity chromatography
Concentration
1mg/ml (lot specific)
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
Molecular Weight
49,953 Da
OMIM
BT019930 mRNA
3D Structure
ModBase 3D Structure for Q9BVA1
Antigen Species
Human
Immunogen
Recombinant human beta-Tubulin (1-445aa) purified from E. coli
Clone Note
Anti-human beta-Tubulin mAb, clone AT5B2, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human beta-Tubulin protein.

Product Categories/Family for anti-TUBB2B antibody

Cytoskeleton
Applications Tested/Suitable for anti-TUBB2B antibody
ELISA (EIA), Western Blot (WB), Immunofluorescence (IF)
Application Notes for anti-TUBB2B antibody
WB/IF: 1:1000 ~ 5000.
Recommended starting dilution is 1:1000

Western Blot (WB) of anti-TUBB2B antibody

The Cell lysate (40ug) and Mouse tissue extracts (35ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human beta-Tubulin (1:5000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Jurkat cell lysate Lane 2.: A549 cell lysate Lane 3.: A431 cell lysate Lane 4.: HepG2 cell lysate Lane 5.: Mouse Brain tissue extract
Mouse Anti-TUBB2B Monoclonal Antibody – Cat# MBS200193

Immunofluorescence (IF) of anti-TUBB2B antibody

Immunofluorescence of HaCaT cells stained with Hoechst 3342 (Blue) for nucleus staining and monoclonal anti-human beta-Tubulin antibody (1:1000) with Texas Red (Red). Immunofluorescence of A431 cells stained with DAPI (Blue) for nucleus staining and monoclonal anti-human beta-Tubulin antibody (1:500) with Alexa Fluor 488 (Green). Immunofluorescence of Balb/3T3 cells stained with DAPI (Blue) for nucleus staining and monoclonal anti-human beta-Tubulin antibody (1:200) with Alexa Fluor 488 (Green).
Mouse Anti-TUBB2B Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS200193

Product References and Citations for anti-TUBB2B antibody

Xavier Hubert Jaglin, et al. (2009) Nat Genet, 41 (6):746-52.; Bouwmeester T, et al. (2004) Nat Cell Bio, 6 (2):97-105.; Modig, C, et al. (1999) Cell Motil Cytoskeleton, 42 (4): 315-330.

Other Notes

Small volumes of anti-TUBB2B antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for TUBB2B purchase

MBS200193 is an antibody targeting TUBB2B. Also known as beta-Tubulin antibody. Reactive in Human, Mouse. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for TUBB2B. It may not necessarily be applicable to this product.

NCBI GI #
29788768
NCBI GeneID
347733
NCBI Accession #
NP_821080
UniProt Primary Accession #
Q9BVA1
UniProt Secondary Accession #
A8K068
UniProt Related Accession #
Q9BVA1
GenBank Nucleotide #
NM_178012.4
NCBI Official Full Name
tubulin beta-2B chain
NCBI Official Synonym Full Names
tubulin beta 2B class IIb
NCBI Official Symbol
TUBB2B
NCBI Official Synonym Symbols
CDCBM7; PMGYSA; bA506K6.1
UniProt Protein Name
Tubulin beta-2B chain
Protein Family
Tubulin
UniProt Gene Name
TUBB2B
NCBI Protein Information
tubulin beta-2B chain

NCBI Summary for TUBB2B

The protein encoded by this gene is a beta isoform of tubulin, which binds GTP and is a major component of microtubules. This gene is highly similar to TUBB2A and TUBB2C. Defects in this gene are a cause of asymmetric polymicrogyria. [provided by RefSeq, Mar 2010]

UniProt Comments for TUBB2B

Tubulin is the major constituent of microtubules (PubMed:23001566, PubMed:28013290, PubMed:26732629). It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain (). Plays a critical role in proper axon guidance in both central and peripheral axon tracts (PubMed:23001566). Implicated in neuronal migration (PubMed:19465910).
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Research Articles on TUBB2B

1. The TUBB2B mutation described here represents an unusual recessive mode of inheritance for missense-mediated tubulinopathies and reinforces the sensitivity of the developing cerebellum to microtubule defects.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-TUBB2B antibody

 Reference Product  PubMed Publications
 TUBA1A antibody  >21 publications with TUBB2B and TUBA1A
 TUBA8 antibody  >8 publications with TUBB2B and TUBA8

Pathways associated with anti-TUBB2B antibody

 Products by Pathway  Pathway Diagram
 Chaperonin-mediated Protein Folding Pathway antibodies  Chaperonin-mediated Protein Folding Pathway Diagram
 Cooperation Of Prefoldin And TriC/CCT In Actin And Tubulin Folding Pathway antibodies  Cooperation Of Prefoldin And TriC/CCT In Actin And Tubulin Folding Pathway Diagram
 Formation Of Tubulin Folding Intermediates By CCT/TriC Pathway antibodies  Formation Of Tubulin Folding Intermediates By CCT/TriC Pathway Diagram
 Gap Junction Pathway antibodies  Gap Junction Pathway Diagram
 Gap Junction Pathway antibodies  Gap Junction Pathway Diagram
 Metabolism Of Proteins Pathway antibodies  Metabolism Of Proteins Pathway Diagram
 Parkin-Ubiquitin Proteasomal System Pathway antibodies  Parkin-Ubiquitin Proteasomal System Pathway Diagram
 Pathogenic Escherichia Coli Infection Pathway antibodies  Pathogenic Escherichia Coli Infection Pathway Diagram
 Pathogenic Escherichia Coli Infection Pathway antibodies  Pathogenic Escherichia Coli Infection Pathway Diagram
 Pathogenic Escherichia Coli Infection Pathway antibodies  Pathogenic Escherichia Coli Infection Pathway Diagram

Diseases associated with anti-TUBB2B antibody

 Disease Name  Pubmed Publications
 Malformations of Cortical Development Antibodies  >25 publications with TUBB2B and Malformations of Cortical Development
 Fibrosis Antibodies  >5 publications with TUBB2B and Fibrosis
 Cognition Disorders Antibodies  >2 publications with TUBB2B and Cognition Disorders
 Neoplasm Metastasis Antibodies  >1 publications with TUBB2B and Neoplasm Metastasis
 Neoplasms, Experimental Antibodies  >1 publications with TUBB2B and Neoplasms, Experimental
 Disease Models, Animal Antibodies  >1 publications with TUBB2B and Disease Models, Animal
 Lung Neoplasms Antibodies  >1 publications with TUBB2B and Lung Neoplasms
 Learning Disorders Antibodies  >1 publications with TUBB2B and Learning Disorders
 Memory Disorders Antibodies  >1 publications with TUBB2B and Memory Disorders

Organs/Tissues associated with anti-TUBB2B antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >35 publications with TUBB2B and Brain
 Ganglia Antibodies  >12 publications with TUBB2B and Ganglia
 Muscle Antibodies  >9 publications with TUBB2B and Muscle
 Nerve Antibodies  >6 publications with TUBB2B and Nerve
 Eye Antibodies  >4 publications with TUBB2B and Eye
 Vascular Antibodies  >2 publications with TUBB2B and Vascular
 Embryonic Tissue Antibodies  >2 publications with TUBB2B and Embryonic Tissue
 Kidney Antibodies  >1 publications with TUBB2B and Kidney
 Intestine Antibodies  >1 publications with TUBB2B and Intestine
 Prostate Antibodies  >1 publications with TUBB2B and Prostate
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