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beta-actin Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS416878

Anti-Beta-Actin is a mouse monoclonal antibody, affinity purified from mouse ascites fluid. More details

$345 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
beta-Actin, Monoclonal Antibody
Full Product Name
Anti-beta-Actin Antibody
Product Gene Name
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
8H10
Host
Mouse
Species Reactivity
Beta-actin from human, mouse, rat, rabbit or chicken. Beta-actin from other species may also be detectable.
Form/Format
Liquid
Purity/Purification
Affinity Purified.
Concentration
100ug/100ul (lot specific)
Specificity
Band size at approximately 40kDA on western blot
Preparation and Storage
This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
Molecular Weight
13,850 Da
Storage Buffer
PBS, pH 7.4 with 0.05% sodium azide.
Immunogen
Beta-actin N-terminal peptide-KLH conjugates.
Applications Tested/Suitable for anti-beta-actin antibody
Western Blot (WB), ELISA (EIA), Dot Blot (DB)
Application Notes for anti-beta-actin antibody
1:1000-10,000 dilution (incubation for one hour at room temperature) for Western blot with ECL.
In order to obtain best results in other assays (ELISA, etc.), please determine optimal working dilution by titration test.

Product References and Citations for anti-beta-actin antibody

Vandal, M et al. "Reduction in DHA transport to the brain of mice expressing human APOE4 compared to APOE2." J. Neurochem. 129:516-26 (2014). PubMed: 24345162.
Lussier, MP et al. "Casein kinase 2 phosphorylates GluA1 and regulates its surface expression." Eur. J. Neurosci. 39:1148-58 (2014). PubMed: 24712994.
Yen, YK et al. "Western blotting by thin-film direct coating." Anal Chem 86:5164-70 (2014). PubMed: 24773468.
Sun, W et al. "Interleukin-1beta promotes hypoxia-induced apoptosis of glioblastoma cells by inhibiting hypoxia-inducible factor-1 mediated adrenomedullin production." Cell Death Dis 5:e1020 (2014). PubMed: 24457964.
Yamada, K et al. " Addition of a single N-glycan to street rabies virus glycoprotein enhances virus production." J Gen Virol 94 (Pt 2):270-5 (2013). PubMed: 23114627. Application: Western Blot.
Balboula, AZ et al. " Cathepsin B activity has a crucial role in the developmental competence of bovine cumulus -oocyte complexes exposed to heat shock during in vitro maturation." Reproduction 146 (4):407-417 (2013). DOI: 10.1530/REP-13-0179. PubMed: 23898216. Application: Western Blot.
Yamada, K et al. "Characterization of street rabies virus variants with an additional N-glycan at position 247 in the glycoprotein." Arch. Virol. 159:207-16 (2013). PubMed: 23921623. Application: Western Blot.
Yamashita, M et al. "Suppression of immunoglobulin production in human peripheral blood mononuclear cells by monocytes via secretion of heavy-chain ferritin." Immunobiology 219:149-57 (2014). PubMed: 24157279. Application: Western Blot.
Kim, BK et al. "Hairless down-regulates expression of Msx2 and its related target genes in hair follicles." J. Dermatol. Sci. 71:203-9 (2013). PubMed: 23702391. Application: Western Blot.
Yatsu, A et al. "Syntaxin-3 is required for melanosomal localization of Tyrp1 in melanocytes." J Invest Dermatol 133:2237-46 (2013). PubMed: 23549422.
Ki, MK et al. "Human antibodies targeting the C-type lectin-like domain of the tumor endothelial cell marker clec14a regulate angiogenic properties in vitro." Oncogene 32:5449-57 (2013). PubMed: 23644659.
Yamada, K et al. "Serial passage of a street rabies virus in mouse neuroblastoma cells resulted in attenuation: potential role of the additional N-glycosylation of a viral glycoprotein in the reduced pathogenicity of street rabies virus." Virus Res 165 (1):34-45 (2012). DOI: 10.1016/j.virusres.2012.01.002. PubMed: 22248643. Application: Western Blot.
Ishibashi, K et al. "Atg16L1, an essential factor for canonical autophagy, participates in hormone secretion from PC12 cells independently of autophagic activit." Mol Biol Cell 23 (16):3193-3202 (2012). DOI: 10.1091/mbc.E12-01-0010. Application: Immunoblot.
Matsui, T et al. "The Rab Interacting Lysosomal Protein (RILP) Homology Domain Functions as a Novel Effector Domain for Small GTPase Rab36." J Biol Chem 287 (34):28619-28631 (2012). DOI: 10.1074/jbc.M112.370544. Application: Western Blot.
Lee, YB et al. "Polydopamine-mediated immobilization of multiple bioactive molecules for the development of functional vascular graft materials." Biomaterials 33 (33):8343-8352 (2012). DOI: 10.1016/j.biomaterials.2012.08.011. Application: Western Blot.
Cho, KA et al. "IL-33 induces Th17-mediated airway inflammation via mast cells in ovalbumin-challenged mice." Am J Physiol Lung Cell Mol Physiol 302 (4):L429-L420 (2012). DOI: 10.1152/ajplung.00252.2011. Application: Western Blot.
Ryu, CH et al. " Valproic acid downregulates the expression of MGMT and sensitizes temozolomide-resistant glioma cells." J Biomed Biotechnol 2012:987495 (2012). DOI: 10.1155/2012/987495. PubMed: 22701311. Application: Western Blot.
St-Amour, I et al. "Impact of intravenous immunoglobulin on the dopaminergic system and immune response in the acute MPTP mouse model of Parkinson's disease." J Neuroinflammation 9:324 (2012). DOI: 10.1186/1742-2094-9-234. Application: Immunoblot.
Kim, BK et al. "Hairless plays a role in formation of inner root sheath via regulation of Dlx3 gene." J. Biol. Chem. 16681-8:287 (2012). PubMed: 22442153. Application: Western Blot.
Ohbayashi, N et al. "The Rab21-GEF activity of Varp, but not its Rab32, 38 effector function, is required for dendrite formation in melanocytes." Mol Biol Cell 23:669-78 (2012). PubMed: 22171327.
Shin, HN et al. "Stromal cell-derived factor-1alpha and macrophage migration-inhibitory factor induce metastatic behavior in CXCR4-expressing colon cancer cells." Int. J. Mol. Med. 30:1537-43 (2012). PubMed: 23023114.
Yamanaka, K et al. "Effects of downregulating DNA methyltransferase 1 transcript by RNA interference on DNA methylation status of the satellite I region and in vitro development of bovine somatic cell nuclear transfer embryos." J Reprod Dev. 57 (3):393-402 (2011). PubMed: 21343670. Application: Western Blot.
Matsui, TS et al. "Non-muscle myosin II induces disassembly of actin stress fibres independently of myosin light chain dephosphorylation." Interface Focus 1:754-766 (2011). DOI: 10.1098/rsfs.2011.0031. PubMed: 23050080. Application: Immunostaining.
Ohta, M et al. "Novel dominant-negative mutant of GATA3 in HDR syndrome." J. Mol. Med. 89:43-50 (2011). PubMed: 21120445.
Balboula, AZ et al. "Cathepsin B activity is related to the quality of bovine cumulus oocyte complexes and its inhibition can improve their developmental competence." Mol Reprod Dev 77 (5):439-448 (2010). DOI: 10.1002/mrd.21164. Application: Western Blot.
Yamanaka, K et al. "Gene silencing of DNA methyltransferases by RNA interference in bovine fibroblast cells." J Reprod Dev. 56 (1):60-67 (2010). PubMed: 19815986. Application: Western Blot.
Yamashiro, Y et al. "Ectopic coexpression of keratin 8 and 18 promotes invasion of transformed keratinocytes and is induced in patients with cutaneous squamous cell carcinoma." Biochem Biophys Res Commun 399 (3):365-372 (2010). DOI: 10.1016/j.bbrc.2010.07.077. PubMed: 20659422. Application: Immunoblot.
Chen, G et al. "The tumor suppressor ING3 is degraded by SCFSkp2-mediated ubiquitin -proteasome system." Oncogene 29 (10):1498-1508 (2010). DOI: 10.1038/onc.2009.424. Application: Western Blot.
Cho, KA et al. "Hypoxia Affected SDF-1alpha-CXCR4 Interaction between Bone Marrow Stem Cells and Osteoblasts via Osteoclast Modulation." Acta Haematol 123 (1):43-47 (2010). DOI: 10.1159/000262750. PubMed: 19955710. Application: Western Blot.
Morisugi, T et al. "Mechanical stretch enhances NF-kappaB-dependent gene expression and poly(ADP-ribose) synthesis in synovial cells." J Biochem 147 (5):633-644 (2010). DOI: 10.1093/jb/mvp210. Application: Western Blot.
Matsui, TS et al. "Actin stress fibers are at a tipping point between conventional shortening and rapid disassembly at physiological levels of MgATP." Biochem Biophys Res Commun. 395 (3):301-306 (2010). DOI: 10.1016/j.bbrc.2010.03.150. PubMed: 20353757. Application: Immunostaining.
Sudo, H et al. "Regulation of apoptosis in nucleus pulposus cells by optimized exogenous Bcl-2 overexpression." J. Orthop. Res. 28:1608-13 (2010). PubMed: 20589931.
Sanz-Clemente, A et al. "Casein kinase 2 regulates the NR2 subunit composition of synaptic NMDA receptors." Neuron 67:984-96 (2010). PubMed: 20869595.
Carpino, N et al. "The Sts proteins target tyrosine phosphorylated, ubiquitinated proteins within TCR signaling pathways." Mol Immunol 46 (16):32241-3231 (2009). DOI: 10.1016/j.molimm.2009.08.015. PubMed: 19733910. Application: Immunoblot.
Suzuki, H et al. "A spontaneous mutation of the Wwox gene and audiogenic seizures in rats with lethal dwarfism and epilepsy." Genes Brain Behav. 8:650-60 (2009). PubMed: 19500159.
Miller, S et al. "A rapid and sensitive reporter gene that uses green fluorescent protein expression to detect chemicals with estrogenic activity." Toxicol Sci 55(1):69-77 (2000). PubMed: 10788561.

Other Notes

Small volumes of anti-beta-actin antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for beta-actin purchase

MBS416878 is an antibody targeting beta-actin. Reactive in Beta-actin from human, mouse, rat, rabbit or chicken. Beta-actin from other species may also be detectable.. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for beta-actin. It may not necessarily be applicable to this product.

NCBI GI #
NCBI Accession #
NCBI Official Full Name
beta-actin, partial
UniProt Protein Name
Beta-actin
UniProt Synonym Protein Names
Beta-actin
UniProt Gene Name
beta-actin
UniProt Entry Name
K4NQ23_9EURO

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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