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TIGAR Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS416896

MBS416896 is an antibody targeting TIGAR. Reactive in Human. Predicted: Mouse, Rat. Host: Rabbit. More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
TIGAR, Polyclonal Antibody
Full Product Name
Anti-TIGAR Antibody
Product Gene Name
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human. Predicted: Mouse, Rat
Form/Format
Liquid
Purity/Purification
Affinity purified
Concentration
100ug/100ul (lot specific)
Preparation and Storage
This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
Molecular Weight
30,063 Da
OMIM
610775
Storage Buffer
PBS, pH 7.4 with 0.02% sodium azide.
Immunogen
Rabbit polyclonal TIGAR(1) antibody was raised against a recombinate human TIGAR protein 2-203aa (BC012340).
Applications Tested/Suitable for anti-TIGAR antibody
ELISA (EIA), Immunohistochemistry (IHC)
Application Notes for anti-TIGAR antibody
ELISA titer: 1:20,000-1:80,000
Immunohistochemistry: 1:100-1:500
Optimal dilutions/concentrations should be determined by the end user

Testing Data of anti-TIGAR antibody

Rabbit Anti-TIGAR Polyclonal Antibody – Cat# MBS416896

Product References and Citations for anti-TIGAR antibody

(1) Wanka,C., Steinbach,J.P. and Rieger,J. Tp53-induced glycolysis and apoptosis regulator (TIGAR) protects glioma cells from starvation-induced cell death by up-regulating respiration and improving cellular redox homeostasis. J. Biol. Chem. 287 (40), 33436-33446 (2012)

Other Notes

Small volumes of anti-TIGAR antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for TIGAR. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
Homo sapiens chromosome 12 open reading frame 5, mRNA
NCBI Official Synonym Full Names
chromosome 12 open reading frame 5
NCBI Official Symbol
C12orf5
NCBI Official Synonym Symbols
FR2BP; TIGAR
UniProt Protein Name
Fructose-2,6-bisphosphatase TIGAR
UniProt Synonym Protein Names
TP53-induced glycolysis and apoptosis regulator
UniProt Gene Name
TIGAR
UniProt Entry Name
TIGAR_HUMAN
NCBI Protein Information
fructose-2,6-bisphosphatase TIGAR; TP53-induced glycolysis and apoptosis regulator; fructose-2,6-bisphosphate 2-phosphatase; probable fructose-2,6-bisphosphatase TIGAR; transactivated by NS3TP2 protein

NCBI Summary for TIGAR

This gene is regulated as part of the p53 tumor suppressor pathway and encodes a protein with sequence similarity to the bisphosphate domain of the glycolytic enzyme that degrades fructose-2,6-bisphosphate. The protein functions by blocking glycolysis and directing the pathway into the pentose phosphate shunt. Expression of this protein also protects cells from DNA damaging reactive oxygen species and provides some protection from DNA damage-induced apoptosis. The 12p13.32 region that includes this gene is paralogous to the 11q13.3 region. [provided by RefSeq, Jul 2008]
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UniProt Comments for TIGAR

TIGAR: Fructose-bisphosphatase hydrolyzing fructose-2,6- bisphosphate as well as fructose-1,6-bisphosphate. Inhibits glycolysis by reducing cellular levels of fructose-2,6- bisphosphate. May protect cells against reactive oxygen species and against apoptosis induced by tp53. Belongs to the phosphoglycerate mutase family.

Protein type: Phosphatase (non-protein); EC 3.1.3.46

Chromosomal Location of Human Ortholog: 12p13.3

Cellular Component: intracellular

Molecular Function: fructose-2,6-bisphosphate 2-phosphatase activity

Biological Process: response to xenobiotic stimulus; dephosphorylation; response to gamma radiation
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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