Rabbit Anti-ESR1 Polyclonal Antibody – Cat# MBS416911

Estrogen Receptor-Alpha (ESR1) Antibody – Rabbit Polyclonal

Name: ESR1 Antibody; Polyclonal; Rabbit; Human/Mouse-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS416911
Price: 510 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS416911

MBS416911 is an antibody targeting ESR1. Also known as Anti-Estrogen Receptor-alpha Antibody. Reactive in Human, Mouse. Host: Rabbit. More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

Estrogen Receptor-alpha (ESR1), Polyclonal Antibody

Full Product Name

Anti-Estrogen Receptor-alpha Antibody

Product Gene Name

anti-ESR1 antibody

Clonality

Polyclonal

Host

Rabbit

Species Reactivity

Human, Mouse

Form/Format

Liquid

Purity/Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1ug/ul (lot specific)

Specificity

Estrogen Receptor-beta (Ab-118) antibody detects endogenous levels of total Estrogen Receptor-beta protein.

Preparation and Storage

This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.

Molecular Weight

35,547 Da

OMIM

133430

Storage Buffer

phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Immunogen

The antiserum was produced against synthesized non-phosphopeptide derived from human Estrogen Receptor-beta around the phosphorylation site of serine118 (Q-L-SP-P-F).

Applications Tested/Suitable for anti-ESR1 antibody

Western Blot (WB), Immunohistochemistry (IHC)

Application Notes for anti-ESR1 antibody

WB: 1:500~1:1000
IHC: 1:50~1:100

Product References and Citations for anti-ESR1 antibody

Medunjanin S, et al. (2005). J Biol Chem.80 (38):33006-33014.
Masuhiro Y, et al. (2005). Proc Natl Acad Sci USA.102 (23): 8126-8131.
Pentecost BT, et al. (2005). Mol Cell Endocrinol.238 (1-2): 9-25.
Park KJ, et al. (2005). Mol Cell.18 (1): 71-82.

Other Notes

Small volumes of anti-ESR1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for ESR1. It may not necessarily be applicable to this product.

NCBI GI #

5821726

NCBI Accession #

AAD52984.1

UniProt Secondary Accession #

Q13511; Q14276; Q5T5H7; Q6MZQ9; Q9NU51; Q9UDZ7; Q9UIS7

NCBI Official Full Name

estrogen receptor alpha, partial

UniProt Protein Name

Estrogen receptor

UniProt Synonym Protein Names

ER-alpha; Estradiol receptor; Nuclear receptor subfamily 3 group A member 1

Protein Family

Estrogen receptor

UniProt Gene Name

ESR1

UniProt Entry Name

ESR1_HUMAN

UniProt Comments for ESR1

ER-alpha: a nuclear hormone receptor and transcription factor. Regulates gene expression and affects cellular proliferation and differentiation in target tissues. Two splice-variant isoforms have been described.

Protein type: DNA-binding; Nuclear receptor

Chromosomal Location of Human Ortholog: 6q25.1

Cellular Component: nucleoplasm; Golgi apparatus; membrane; cytoplasm; plasma membrane; integral to membrane; nucleus

Molecular Function: identical protein binding; estrogen receptor activity; zinc ion binding; nitric-oxide synthase regulator activity; beta-catenin binding; estrogen response element binding; transcription factor binding; steroid binding; protein binding; enzyme binding; steroid hormone receptor activity; chromatin binding; transcription factor activity

Biological Process: positive regulation of nitric oxide biosynthetic process; estrogen receptor signaling pathway; signal transduction; uterus development; response to estradiol stimulus; regulation of apoptosis; elevation of cytosolic calcium ion concentration; positive regulation of fibroblast proliferation; regulation of transcription, DNA-dependent; epithelial cell development; steroid hormone receptor signaling pathway; transcription initiation from RNA polymerase II promoter; transcription, DNA-dependent; negative regulation of transcription factor activity; antral ovarian follicle growth; positive regulation of nitric-oxide synthase activity; androgen metabolic process; male gonad development; vagina development; positive regulation of retinoic acid receptor signaling pathway; negative regulation of I-kappaB kinase/NF-kappaB cascade; chromatin remodeling; response to estrogen stimulus; steroid hormone mediated signaling; gene expression; positive regulation of transcription factor activity; G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); positive regulation of transcription from RNA polymerase II promoter

Disease: Breast Cancer; Migraine With Or Without Aura, Susceptibility To, 1; Myocardial Infarction, Susceptibility To; Estrogen Resistance

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.