MOCS3 Antibody – Rabbit Polyclonal

Molybdenum cofactor (MoCo) is necessary for the function of all molybdoenzymes. The protein encoded by this gene adenylates and activates molybdopterin synthase, an enzyme required for biosynthesis of MoCo. This gene contains no introns. A pseudogene of this gene is present on chromosome 14. [provided by RefSeq, Nov 2012]

MOCS3: Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). Also essential during biosynthesis of the molybdenum cofactor. Acts by mediating the C-terminal thiocarboxylation of sulfur carriers URM1 and MOCS2A. Its N-terminus first activates URM1 and MOCS2A as acyl-adenylates (-COAMP), then the persulfide sulfur on the catalytic cysteine is transferred to URM1 and MOCS2A to form thiocarboxylation (-COSH) of their C-terminus. The reaction probably involves hydrogen sulfide that is generated from the persulfide intermediate and that acts as nucleophile towards URM1 and MOCS2A. Subsequently, a transient disulfide bond is formed. Does not use thiosulfate as sulfur donor; NFS1 probably acting as a sulfur donor for thiocarboxylation reactions.

Protein type: EC 2.7.7.80; Ligase; Transferase; EC 2.8.1.11

Chromosomal Location of Human Ortholog: 20q13.13

Cellular Component: cytosol

Molecular Function: sulfurtransferase activity; protein binding; thiosulfate sulfurtransferase activity; metal ion binding; ATP binding; URM1 activating enzyme activity; nucleotidyltransferase activity

Biological Process: protein urmylation; tRNA wobble uridine modification; vitamin metabolic process; enzyme active site formation via L-cysteine persulfide; protein amino acid adenylylation; Mo-molybdopterin cofactor biosynthetic process; molybdopterin cofactor biosynthetic process; water-soluble vitamin metabolic process