Rabbit Anti-GCAT Polyclonal Antibody – Cat# MBS417447

GCAT Antibody – Rabbit Polyclonal

Name: GCAT Antibody; Polyclonal; Rabbit; Human/Rat/Mouse-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS417447
Price: 335 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS417447

The degradation of L-threonine to glycine consists of a two-step biochemical pathway involving the enzymes L-threonine dehydrogenase and 2-amino-3-ketobutyrate coenzyme A ligase. L-Threonine is first converted into 2-amino-3-ketobutyrate by L-threonine… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

GCAT, Polyclonal Antibody

Full Product Name

Anti-GCAT Antibody

Product Gene Name

anti-GCAT antibody

Clonality

Polyclonal

Host

Rabbit

Species Reactivity

Human. Predicted: Rat, Mouse. Not yet tested in other species

Form/Format

Liquid

Purity/Purification

Affinity purified

Concentration

100ug/100ul (lot specific)

Preparation and Storage

This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.

Molecular Weight

47,974 Da

OMIM

607422

Storage Buffer

PBS, pH 7.4 with 0.02% sodium azide.

Immunogen

Rabbit polyclonal GCAT (1) antibody was raised against a recombinate human GCAT protein 45-209 aa (BC014457).

Applications Tested/Suitable for anti-GCAT antibody

ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)

Application Notes for anti-GCAT antibody

ELISA titer: 1:20,000-1:80,000
Western blot analysis: 1:200-1:1,000
Immunohistochemistry: 1:100-1:500
Optimal dilutions/concentrations should be determined by the end user

Product References and Citations for anti-GCAT antibody

(1) Hendrickson,S.L., Lautenberger,J.A., Chinn,L.W., et al. Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression. PLoS ONE 5 (9), E12862 (2010)

Other Notes

Small volumes of anti-GCAT antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for GCAT purchase

MBS417447 is an antibody targeting GCAT. Reactive in Human. Predicted: Rat, Mouse. Not yet tested in other species. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for GCAT. It may not necessarily be applicable to this product.

NCBI GI #

15680211

NCBI GeneID

23464

NCBI Accession #

BC014457

UniProt Secondary Accession #

Q6ZWF1; Q96CA9; E2QC23

UniProt Related Accession #

O75600

NCBI Official Full Name

Homo sapiens glycine C-acetyltransferase (2-amino-3-ketobutyrate coenzyme A ligase), mRNA

NCBI Official Synonym Full Names

glycine C-acetyltransferase

NCBI Official Symbol

GCAT

NCBI Official Synonym Symbols

KBL

UniProt Protein Name

2-amino-3-ketobutyrate coenzyme A ligase, mitochondrial

UniProt Synonym Protein Names

Aminoacetone synthase; Glycine acetyltransferase

Protein Family

Phosphatidylcholine-sterol acyltransferase

UniProt Gene Name

GCAT

UniProt Entry Name

KBL_HUMAN

NCBI Protein Information

2-amino-3-ketobutyrate coenzyme A ligase, mitochondrial; 2-amino-3-ketobutyrate-CoA ligase; AKB ligase; aminoacetone synthase; glycine acetyltransferase

UniProt Comments for GCAT

GCAT: The degradation of L-threonine to glycine consists of a two-step biochemical pathway involving the enzymes L-threonine dehydrogenase and 2-amino-3-ketobutyrate coenzyme A ligase. L-Threonine is first converted into 2-amino-3-ketobutyrate by L-threonine dehydrogenase. This gene encodes the second enzyme in this pathway, which then catalyzes the reaction between 2-amino-3-ketobutyrate and coenzyme A to form glycine and acetyl-CoA. The encoded enzyme is considered a class II pyridoxal-phosphate-dependent aminotransferase. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 14. [provided by RefSeq, Jan 2010]

Protein type: Transferase; Mitochondrial; Amino Acid Metabolism - glycine, serine and threonine; EC 2.3.1.29

Chromosomal Location of Human Ortholog: 22q13.1

Cellular Component: nucleoplasm; mitochondrion

Molecular Function: glycine C-acetyltransferase activity; pyridoxal phosphate binding

Biological Process: amino acid metabolic process; biosynthetic process; threonine catabolic process to pyruvate

Research Articles on GCAT

1. Observational study of gene-disease association. (HuGE Navigator)

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-GCAT antibody

Products by Pathway	Pathway Diagram
Glycine, Serine And Threonine Metabolism Pathway antibodies	Glycine, Serine And Threonine Metabolism Pathway Diagram
Glycine, Serine And Threonine Metabolism Pathway antibodies	Glycine, Serine And Threonine Metabolism Pathway Diagram

Diseases associated with anti-GCAT antibody

Disease Name	Pubmed Publications
Hypertension Antibodies	>1 publications with GCAT and Hypertension
Atherosclerosis Antibodies	>1 publications with GCAT and Atherosclerosis
Edema Antibodies	>1 publications with GCAT and Edema
Congenital Abnormalities Antibodies	>1 publications with GCAT and Congenital Abnormalities
Hemorrhage Antibodies	>1 publications with GCAT and Hemorrhage
Kidney Diseases Antibodies	>1 publications with GCAT and Kidney Diseases
Inflammation Antibodies	>1 publications with GCAT and Inflammation

Organs/Tissues associated with anti-GCAT antibody

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>7 publications with GCAT and Blood
Kidney Antibodies	>3 publications with GCAT and Kidney
Bone Antibodies	>3 publications with GCAT and Bone
Brain Antibodies	>3 publications with GCAT and Brain
Skin Antibodies	>2 publications with GCAT and Skin
Eye Antibodies	>2 publications with GCAT and Eye
Nerve Antibodies	>1 publications with GCAT and Nerve
Thymus Antibodies	>1 publications with GCAT and Thymus
Embryonic Tissue Antibodies	>1 publications with GCAT and Embryonic Tissue
Heart Antibodies	>1 publications with GCAT and Heart