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RFC1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS419735

This gene encodes the large subunit of replication factor C, a five subunit DNA polymerase accessory protein, which is a DNA-dependent ATPase required for eukaryotic DNA replication and repair. The large subunit acts as an activator of DNA polymerases, binds… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
RFC1, Polyclonal Antibody
Full Product Name
Anti-RFC1 Antibody
Product Gene Name
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Liquid
Purity/Purification
Affinity Purification
Concentration
1mg/ml (lot specific)
Specificity
Western blot analysis of extracts of MCF-7 cell lines, using RFC1 antibody. Immunohistochemistry of paraffin-embedded human lung cancer using RFC1 antibody at dilution of 1:200 (400x lens). Immunohistochemistry of paraffin-embedded rat lung using RFC1 antibody at dilution of 1:200 (400x lens). Immunofluorescence analysis of MCF7 cell using RFC1 antibody. Blue: DAPI for nuclear staining.
Preparation and Storage
Store at -20 degree C (regular) and -80 degree C (long term). Avoid freeze / thaw cycles.
Molecular Weight
94,903 Da
Immunogen
Recombinant protein of human RFC1
Storage Buffer
PBS with 0.02% sodium azide,50% glycerol, pH7.3.
Applications Tested/Suitable for anti-RFC1 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes for anti-RFC1 antibody
WB: 1:500 - 1:2000
IHC: 1:50- 1:200
IF: 1:50- 1:200

Western Blot (WB) of anti-RFC1 antibody

Rabbit Anti-RFC1 Polyclonal Antibody – Cat# MBS419735

Immunohistochemistry (IHC) of anti-RFC1 antibody

Rabbit Anti-RFC1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS419735

Immunohistochemistry (IHC) of anti-RFC1 antibody

Rabbit Anti-RFC1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS419735

Immunofluorescence (IF) of anti-RFC1 antibody

Rabbit Anti-RFC1 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS419735

Other Notes

Small volumes of anti-RFC1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for RFC1 purchase

MBS419735 is an antibody targeting RFC1. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for RFC1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI Accession #
UniProt Secondary Accession #
NCBI Official Full Name
RFC1
UniProt Protein Name
Replication factor C subunit 1
UniProt Synonym Protein Names
Activator 1 95 kDa subunit; Cell division control protein 44
UniProt Gene Name
RFC1
UniProt Synonym Gene Names
CDC44; Replication factor C1

UniProt Comments for RFC1

Component of the ATP-dependent clamp loader RFC complex for the POL30/PCNA homotrimer DNA clamp. During a clamp loading circle, the RFC:clamp complex binds to DNA and the recognition of the double-stranded/single-stranded junction stimulates ATP hydrolysis by RFC. The complex presumably provides bipartite ATP sites in which one subunit supplies a catalytic site for hydrolysis of ATP bound to the neighboring subunit. Dissociation of RFC from the clamp leaves the clamp encircling DNA. Replication factor C (RFC or activator 1) complex acts during elongation of primed DNA templates by DNA polymerase delta and epsilon. RFC has an essential but redundant activity in sister chromatid cohesion establishment.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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