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4e-T/Eif4enif1 (EIF4ENIF1) Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS420001

MBS420001 is an antibody targeting EIF4ENIF1. Also known as Goat anti-4E-T/EIF4ENIF1 Antibody. Reactive in Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow. Host: Goat. More details

$450 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
4E-T/EIF4ENIF1, Polyclonal Antibody
Full Product Name
Goat anti-4E-T/EIF4ENIF1 Antibody
Product Gene Name
Product Synonym Gene Name
4E-T
Product Synonym Names
EIF4ENIF1; 4E-T; Clast4; FLJ21601; 2610509L04Rik; eukaryotic translation initiation factor 4E nuclear import factor 1; eIF4E-transporter; FLJ26551; OTTHUMP00000063276; EIF4ENIF1 antibody; 4E-T antibody; Clast4 antibody; FLJ21601 antibody; 2610509L04Rik antibody; eukaryotic translation initiation factor 4E nuclear import factor 1 antibody; eIF4E-transporter antibody; FLJ26551 antibody; OTTHUMP00000063276 antibody
Immunogen Sequence
AKVISVDELE YRQ
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
100ug specific antibody in 200ul (lot specific)
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Antibody/Peptide Pairs
Molecular Weight
108,329 Da
OMIM
607445
Immunogen
Peptide with sequence C-AKVISVDELEYRQ, from the C Terminus of the protein sequence according to NP_062817.2; NP_001157974.1.
Epitope
C Terminus
Note
This antibody is expected to recognise isoform 1 (NP_062817.2) and isoform b (NP_001157974.1). Reported variants represent identical protein (NP_062817.2; NP_001157973.1).
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Applications Tested/Suitable for anti-EIF4ENIF1 antibody
Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-EIF4ENIF1 antibody
Peptide ELISA: Antibody detection limit dilution 1: 32000.
Immunohistochemistry: In paraffin embedded Human Pancreas shows strong staining of cytoplasm in acinar cells-. Recommended concentration, 5-10ug/ml.
Western Blot: Approx 140-150kDa band observed in 293 lysates (predicted size of approx.108kDa according to NP_062817.1, however our observation agrees with that of Dostie et al (see below)). Recommended for use at 0.25-0.5ug/ml.

Western Blot (WB) of anti-EIF4ENIF1 antibody

Staining (0.25ug/ml) of 293 lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Goat Anti-EIF4ENIF1 Polyclonal Antibody – Cat# MBS420001

Immunohistochemistry (IHC) of anti-EIF4ENIF1 antibody

(5ug/ml) staining of paraffin embedded Human Pancreas. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Goat Anti-EIF4ENIF1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS420001

Other Notes

Small volumes of anti-EIF4ENIF1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for EIF4ENIF1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI Related Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
eukaryotic translation initiation factor 4E transporter isoform a
NCBI Official Synonym Full Names
eukaryotic translation initiation factor 4E nuclear import factor 1
NCBI Official Symbol
EIF4ENIF1
NCBI Official Synonym Symbols
4E-T; Clast4
UniProt Protein Name
Eukaryotic translation initiation factor 4E transporter
UniProt Synonym Protein Names
Eukaryotic translation initiation factor 4E nuclear import factor 1
UniProt Gene Name
EIF4ENIF1
UniProt Synonym Gene Names
4E-T; eIF4E transporter
UniProt Entry Name
4ET_HUMAN
NCBI Protein Information
eukaryotic translation initiation factor 4E transporter

NCBI Summary for EIF4ENIF1

The protein encoded by this gene is a nucleocytoplasmic shuttle protein for the translation initiation factor eIF4E. This shuttle protein interacts with the importin alpha-beta complex to mediate nuclear import of eIF4E. It is predominantly cytoplasmic; its own nuclear import is regulated by a nuclear localization signal and nuclear export signals. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2009]
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UniProt Comments for EIF4ENIF1

4E-T: Nucleoplasmic shuttling protein. Mediates the nuclear import of EIF4E by a piggy-back mechanism. Interacts with EIF4E. Interacts with importin beta only in the presence of importin alpha, suggesting a direct interaction with importin alpha. Widely expressed. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Karyopherin; Nuclear import; Translation

Chromosomal Location of Human Ortholog: 22q11.2

Cellular Component: cytoplasm; intracellular membrane-bound organelle; membrane; nucleus

Molecular Function: protein binding; protein transporter activity
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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