Goat Anti-ARFGAP3 Polyclonal Antibody – Cat# MBS420038

Staining (2ug/ml) of mouse brain extracts (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

ARFGAP3 Antibody – Goat Polyclonal

Name: ARFGAP3 Antibody; Polyclonal; Goat; Mouse/Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS420038
Price: 450 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS420038

MBS420038 is an antibody targeting ARFGAP3. Also known as Goat anti-ARFGAP3 Antibody. Reactive in Tested: Mouse; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow. Host: Goat. More details

$450 / 0.1 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

ARFGAP3, Polyclonal Antibody

Full Product Name

Goat anti-ARFGAP3 Antibody

Product Gene Name

anti-ARFGAP3 antibody

Product Synonym Names

ARFGAP3; ADP-ribosylation factor GTPase activating protein 3; ARFGAP1; RP1-47A17.3; ADP-ribosylation factor GTPase activating protein 1; OTTHUMP00000028526; FLJ45618; ARFGAP3 antibody; ADP-ribosylation factor GTPase activating protein 3 antibody; ARFGAP1 antibody; RP1-47A17.3 antibody; ADP-ribosylation factor GTPase activating protein 1 antibody; OTTHUMP00000028526 antibody; FLJ45618 antibody

Immunogen Sequence

NGVVTSIQDR YGS

Clonality

Polyclonal

Host

Goat

Species Reactivity

Tested: Mouse; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow

Form/Format

Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Purity/Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

100ug specific antibody in 200ul (lot specific)

Preparation and Storage

Aliquot and store at -20 degree C. Minimize freezing and thawing.

Antibody/Peptide Pairs

ARFGAP3 peptide (MBS426317) is used for blocking the activity of ARFGAP3 antibody (MBS420038)

Molecular Weight

51,990 Da

OMIM

612439

Immunogen

Peptide with sequence C-NGVVTSIQDRYGS, from the C Terminus of the protein sequence according to NP_055385.3; NP_001135765.1.

Epitope

C Terminus

Note

This antibody is expected to recognize both reported isoforms (NP_055385.3; NP_001135765.1).

ISO Certification

Manufactured in an ISO 9001:2008 Certified Laboratory.

Applications Tested/Suitable for anti-ARFGAP3 antibody

Peptide ELISA (EIA), Western Blot (WB)

Application Notes for anti-ARFGAP3 antibody

Peptide ELISA: Antibody detection limit dilution 1: 8000.
Western Blot: Approx 55-60kDa band seen in mouse brain extracts. Recommended for use at 1-3ug/ml.

Other Notes

Small volumes of anti-ARFGAP3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for ARFGAP3. It may not necessarily be applicable to this product.

NCBI GI #

214829673

NCBI GeneID

26286

NCBI Accession #

NP_055385.3

NCBI Related Accession #

NP_001135765.1

UniProt Secondary Accession #

Q9BSC6; Q9H9J0; Q9NT10; Q9NUP5; Q9Y4V3; Q9Y4V4; E9PB03

UniProt Related Accession #

Q9NP61

GenBank Nucleotide #

NM_014570.4

NCBI Official Full Name

ADP-ribosylation factor GTPase-activating protein 3 isoform 1

NCBI Official Synonym Full Names

ADP ribosylation factor GTPase activating protein 3

NCBI Official Symbol

ARFGAP3

NCBI Official Synonym Symbols

ARFGAP1

UniProt Protein Name

ADP-ribosylation factor GTPase-activating protein 3

Protein Family

ADP-ribosylation factor GTPase-activating protein

UniProt Gene Name

ARFGAP3

UniProt Synonym Gene Names

ARFGAP1; ARF GAP 3

UniProt Entry Name

ARFG3_HUMAN

NCBI Protein Information

ADP-ribosylation factor GTPase-activating protein 3

NCBI Summary for ARFGAP3

The protein encoded by this gene is a GTPase-activating protein (GAP) that associates with the Golgi apparatus and regulates the early secretory pathway of proteins. The encoded protein promotes hydrolysis of ADP-ribosylation factor 1 (ARF1)-bound GTP, which is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles. Dissociation of the coat proteins is a prerequisite for the fusion of these vesicles with target compartments. The activity of this protein is sensitive to phospholipids. Multiple transcript variants encoding different isoforms have been found for this gene. This gene was originally known as ARFGAP1, but that is now the name of a related but different gene. [provided by RefSeq, Nov 2008]

UniProt Comments for ARFGAP3

ARF GAP 3: GTPase-activating protein (GAP) for ADP ribosylation factor 1 (ARF1). Hydrolysis of ARF1-bound GTP may lead to dissociation of coatomer from Golgi-derived membranes to allow fusion with target membranes.

Protein type: GAPs; GAPs, ARF

Chromosomal Location of Human Ortholog: 22q13.2

Cellular Component: cytoplasm; cytosol; Golgi apparatus; membrane

Molecular Function: GTPase activator activity; protein binding

Biological Process: ER to Golgi vesicle-mediated transport; protein secretion; retrograde vesicle-mediated transport, Golgi to ER

Research Articles on ARFGAP3

1. We suggest a model in which ArfGAP3 regulates Golgi association of GGA clathrin adaptors.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-ARFGAP3 antibody

Products by Pathway	Pathway Diagram
Asparagine N-linked Glycosylation Pathway antibodies	Asparagine N-linked Glycosylation Pathway Diagram
COPI-dependent Golgi-to-ER Retrograde Traffic Pathway antibodies	COPI-dependent Golgi-to-ER Retrograde Traffic Pathway Diagram
COPI-mediated Anterograde Transport Pathway antibodies	COPI-mediated Anterograde Transport Pathway Diagram
ER To Golgi Anterograde Transport Pathway antibodies	ER To Golgi Anterograde Transport Pathway Diagram
Endocytosis Pathway antibodies	Endocytosis Pathway Diagram
Endocytosis Pathway antibodies	Endocytosis Pathway Diagram
Golgi-to-ER Retrograde Transport Pathway antibodies	Golgi-to-ER Retrograde Transport Pathway Diagram
Intra-Golgi And Retrograde Golgi-to-ER Traffic Pathway antibodies	Intra-Golgi And Retrograde Golgi-to-ER Traffic Pathway Diagram
Membrane Trafficking Pathway antibodies	Membrane Trafficking Pathway Diagram
Metabolism Of Proteins Pathway antibodies	Metabolism Of Proteins Pathway Diagram

Organs/Tissues associated with anti-ARFGAP3 antibody

Organ/Tissue Name	Pubmed Publications
Eye Antibodies	>2 publications with ARFGAP3 and Eye
Ovary Antibodies	>2 publications with ARFGAP3 and Ovary
Prostate Antibodies	>2 publications with ARFGAP3 and Prostate
Testis Antibodies	>2 publications with ARFGAP3 and Testis
Lung Antibodies	>1 publications with ARFGAP3 and Lung
Blood Antibodies	>1 publications with ARFGAP3 and Blood
Liver Antibodies	>1 publications with ARFGAP3 and Liver