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PLRG1 Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS420049

MBS420049 is an antibody targeting PLRG1. Also known as Goat anti-PLRG1 Antibody. Reactive in Tested: Human, Mouse; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow. Host: Goat. More details

$450 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
PLRG1, Polyclonal Antibody
Full Product Name
Goat anti-PLRG1 Antibody
Product Gene Name
Product Synonym Names
PLRG1; pleiotropic regulator 1 (PRL1 homolog, Arabidopsis); PRL1; MGC110980; pleiotropic regulator 1 (PRL1, Arabidopsis homolog); pleiotropic regulator 1 (PRL1homolog, Arabidopsis); pleiotropic regulator 1 (PRL1, Arabidopsis homolog); PLRG1 antibody; pleiotropic regulator 1 (PRL1 homolog; Arabidopsis) antibody; PRL1 antibody; MGC110980 antibody; pleiotropic regulator 1 (PRL1; Arabidopsis homolog) antibody; pleiotropic regulator 1 (PRL1homolog; Arabidopsis) antibody; pleiotropic regulator 1 (PRL1; Arabidopsis homolog) antibody
Immunogen Sequence
PVSWKPEIIK RKRF
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human, Mouse; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
100ug specific antibody in 200ul (lot specific)
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Antibody/Peptide Pairs
PLRG1 peptide (MBS426231) is used for blocking the activity of PLRG1 antibody (MBS420049)
Molecular Weight
56,290 Da
OMIM
605961
Immunogen
Peptide with sequence C-PVSWKPEIIKRKRF, from the C Terminus of the protein sequence according to NP_002660.1; NP_001188493.1.
Epitope
C Terminus
Note
This antibody is expected to recognise isoform 1 (NP_002660.1) and isoform 2 (NP_001188493.1).
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Applications Tested/Suitable for anti-PLRG1 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes for anti-PLRG1 antibody
Peptide ELISA: Antibody detection limit dilution 1: 16000.
Western Blot: Approx 60kDa band observed in nuclear lysates of cell lines HEK293 and NIH3T3 (calculated MW of 57.2kDa according to Human NP_002660.1 and 56.9kDa according to Mouse NP_058064.2). Recommended concentration: 0.1-0.3ug/ml.

Western Blot (WB) of anti-PLRG1 antibody

(0.1ug/ml) staining of HEK293 (A) and NIH3T3 (B) nuclear cell lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Goat Anti-PLRG1 Polyclonal Antibody – Cat# MBS420049

Other Notes

Small volumes of anti-PLRG1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for PLRG1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI Related Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
pleiotropic regulator 1 isoform 1
NCBI Official Synonym Full Names
pleiotropic regulator 1
NCBI Official Symbol
PLRG1
NCBI Official Synonym Symbols
Cwc1; PRL1; PRP46; PRPF46; TANGO4
UniProt Protein Name
Pleiotropic regulator 1
Protein Family
UniProt Gene Name
PLRG1
UniProt Entry Name
PLRG1_HUMAN
NCBI Protein Information
pleiotropic regulator 1

NCBI Summary for PLRG1

This gene encodes a core component of the cell division cycle 5-like (CDC5L) complex. The CDC5L complex is part of the spliceosome and is required for pre-mRNA splicing. The encoded protein plays a critical role in alternative splice site selection. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jan 2011]
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UniProt Comments for PLRG1

PLRG1: Component of the PRP19-CDC5L complex that forms an integral part of the spliceosome and is required for activating pre-mRNA splicing. Belongs to the WD repeat PRL1/PRL2 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription, coactivator/corepressor; RNA splicing; Spliceosome

Chromosomal Location of Human Ortholog: 4q31.2-q32.1

Cellular Component: DNA replication factor A complex; nuclear membrane; nuclear speck; nucleolus; nucleoplasm; nucleus

Molecular Function: protein binding

Biological Process: nuclear mRNA splicing, via spliceosome
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Diseases associated with anti-PLRG1 antibody

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