Goat Anti-PPIA Polyclonal Antibody – Cat# MBS420056

(0.1ug/ml) staining of Human Lymph lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Cyclophilin A/PPIA (PPIA) Antibody – Goat Polyclonal

Name: PPIA Antibody; Polyclonal; Goat; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS420056
Price: 450 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS420056

MBS420056 is an antibody targeting PPIA. Also known as Goat anti-Cyclophilin A/PPIA Antibody. Reactive in Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow. Host: Goat. More details

$450 / 0.1 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Cyclophilin A/PPIA, Polyclonal Antibody

Full Product Name

Goat anti-Cyclophilin A/PPIA Antibody

Product Gene Name

anti-PPIA antibody

Product Synonym Names

PPIA; peptidylprolyl isomerase A (cyclophilin A); HGNC: 9253; CYPA; CYPH; MGC12404; T cell cyclophilin; cyclosporin A-binding protein; peptidyl-prolyl cis-trans isomerase A; peptidylprolyl isomerase A; rotamase; MGC117158; MGC23397; PPIase A; PPIA antibody; peptidylprolyl isomerase A (cyclophilin A) antibody; HGNC: 9253 antibody; CYPA antibody; CYPH antibody; MGC12404 antibody; T cell cyclophilin antibody; cyclosporin A-binding protein antibody; peptidyl-prolyl cis-trans isomerase A antibody; peptidylprolyl isomerase A antibody; rotamase antibody; MGC117158 antibody; MGC23397 antibody; PPIase A antibody; Cyclophilin A; Cyclophilin A / PPIA

Immunogen Sequence

ERFGSRNGKT SKK

Clonality

Polyclonal

Host

Goat

Species Reactivity

Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow

Form/Format

Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Purity/Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

100ug specific antibody in 200ul (lot specific)

Preparation and Storage

Aliquot and store at -20 degree C. Minimize freezing and thawing.

Antibody/Peptide Pairs

Cyclophilin A / PPIA peptide (MBS426780) is used for blocking the activity of Cyclophilin A/PPIA antibody (MBS420056)

Molecular Weight

11,418 Da

OMIM

123840

Immunogen

Peptide with sequence C-ERFGSRNGKTSKK, from the internal region of the protein sequence according to NP_066953.1.

Epitope

Internal region

Note

This product is expected to also recognise PPIAL (NP_001008741.1, GeneID: 388817) which is identical to PPIA (NP_066953.1).

ISO Certification

Manufactured in an ISO 9001:2008 Certified Laboratory.

Applications Tested/Suitable for anti-PPIA antibody

Peptide ELISA (EIA), Western Blot (WB)

Application Notes for anti-PPIA antibody

Peptide ELISA: Antibody detection limit dilution 1: 8000.
Western Blot: Approx.18kDa band observed in Human Lymph Node lysates (calculated MW 18. 0kDa according to NP_066953.1), Recommended Concetration: 0.1-0.3ug/ml.

Other Notes

Small volumes of anti-PPIA antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for PPIA. It may not necessarily be applicable to this product.

NCBI GI #

10863927

NCBI GeneID

5478

NCBI Accession #

NP_066953.1

UniProt Secondary Accession #

P05092; Q3KQW3; Q567Q0; Q6IBU5; Q96IX3; Q9BRU4; Q9BTY9; Q9UC61; A8K220

UniProt Related Accession #

P62937

GenBank Nucleotide #

NM_021130.4

NCBI Official Full Name

peptidyl-prolyl cis-trans isomerase A isoform 1

NCBI Official Synonym Full Names

peptidylprolyl isomerase A

NCBI Official Symbol

PPIA

NCBI Official Synonym Symbols

CYPA; CYPH; HEL-S-69p

UniProt Protein Name

Peptidyl-prolyl cis-trans isomerase A

UniProt Synonym Protein Names

Cyclophilin A; Cyclosporin A-binding protein; Rotamase A

Protein Family

Peptidyl-prolyl cis-trans isomerase

UniProt Gene Name

PPIA

UniProt Synonym Gene Names

CYPA; PPIase A

UniProt Entry Name

PPIA_HUMAN

NCBI Protein Information

peptidyl-prolyl cis-trans isomerase A

NCBI Summary for PPIA

This gene encodes a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. The encoded protein is a cyclosporin binding-protein and may play a role in cyclosporin A-mediated immunosuppression. The protein can also interact with several HIV proteins, including p55 gag, Vpr, and capsid protein, and has been shown to be necessary for the formation of infectious HIV virions. Multiple pseudogenes that map to different chromosomes have been reported. [provided by RefSeq, Jul 2008]

UniProt Comments for PPIA

PPIA: PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. Interacts with HIV-1 Capsid protein. Binds cyclosporin A (CsA). CsA mediates some of its effects via an inhibitory action on PPIase. Belongs to the cyclophilin-type PPIase family. PPIase A subfamily.

Protein type: EC 5.2.1.8; RNA-binding; Isomerase; Cyclophilin

Chromosomal Location of Human Ortholog: 7p13

Cellular Component: cytosol; extracellular region; extracellular space; focal adhesion; membrane; nucleus

Molecular Function: peptide binding; peptidyl-prolyl cis-trans isomerase activity; protein binding; unfolded protein binding; virion binding

Biological Process: entry into host cell; leukocyte migration; positive regulation of protein secretion; positive regulation of viral genome replication; protein folding; protein peptidyl-prolyl isomerization; regulation of viral genome replication; release of virus from host; RNA-dependent DNA replication; uncoating of virus; viral envelope fusion with host membrane; viral infectious cycle; virus assembly

Research Articles on PPIA

1. Extracellular CyPA activates platelets via cluster of differentiation 147-mediated phosphoinositid-3-kinase/Akt-signaling, leading to enhanced adhesion and thrombus formation independently of intracellular CyPA.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-PPIA antibody

Reference Product	PubMed Publications
UBC antibody	>23 publications with PPIA and UBC
CFL1 antibody	>3 publications with PPIA and CFL1
TAGLN2 antibody	>2 publications with PPIA and TAGLN2
PFN1 antibody	>1 publications with PPIA and PFN1
MAPK1 antibody	>1 publications with PPIA and MAPK1

Pathways associated with anti-PPIA antibody

Products by Pathway	Pathway Diagram
APOBEC3G Mediated Resistance To HIV-1 Infection Pathway antibodies	APOBEC3G Mediated Resistance To HIV-1 Infection Pathway Diagram
Assembly Of The HIV Virion Pathway antibodies	Assembly Of The HIV Virion Pathway Diagram
Basigin Interactions Pathway antibodies	Basigin Interactions Pathway Diagram
Binding And Entry Of HIV Virion Pathway antibodies	Binding And Entry Of HIV Virion Pathway Diagram
Budding And Maturation Of HIV Virion Pathway antibodies	Budding And Maturation Of HIV Virion Pathway Diagram
Cell Surface Interactions At The Vascular Wall Pathway antibodies	Cell Surface Interactions At The Vascular Wall Pathway Diagram
Disease Pathway antibodies	Disease Pathway Diagram
Early Phase Of HIV Life Cycle Pathway antibodies	Early Phase Of HIV Life Cycle Pathway Diagram
HIV Infection Pathway antibodies	HIV Infection Pathway Diagram
HIV Life Cycle Pathway antibodies	HIV Life Cycle Pathway Diagram

Diseases associated with anti-PPIA antibody

Disease Name	Pubmed Publications
Inflammation Antibodies	>18 publications with PPIA and Inflammation
Cardiovascular Diseases Antibodies	>18 publications with PPIA and Cardiovascular Diseases
Necrosis Antibodies	>14 publications with PPIA and Necrosis
Heart Diseases Antibodies	>14 publications with PPIA and Heart Diseases
Neoplasms, Experimental Antibodies	>13 publications with PPIA and Neoplasms, Experimental
Lung Diseases Antibodies	>9 publications with PPIA and Lung Diseases
Nervous System Diseases Antibodies	>9 publications with PPIA and Nervous System Diseases
Breast Neoplasms Antibodies	>8 publications with PPIA and Breast Neoplasms
Ovarian Neoplasms Antibodies	>7 publications with PPIA and Ovarian Neoplasms
Brain Diseases Antibodies	>7 publications with PPIA and Brain Diseases

Organs/Tissues associated with anti-PPIA antibody

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>36 publications with PPIA and Blood
Muscle Antibodies	>26 publications with PPIA and Muscle
Brain Antibodies	>21 publications with PPIA and Brain
Heart Antibodies	>17 publications with PPIA and Heart
Liver Antibodies	>14 publications with PPIA and Liver
Bone Antibodies	>14 publications with PPIA and Bone
Lung Antibodies	>14 publications with PPIA and Lung
Kidney Antibodies	>13 publications with PPIA and Kidney
Vascular Antibodies	>11 publications with PPIA and Vascular
Adipose Tissue Antibodies	>9 publications with PPIA and Adipose Tissue