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WFDC1 Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS420096

MBS420096 is an antibody targeting WFDC1. Also known as Goat anti-WFDC1/PS20 Antibody. Reactive in Expected from sequence similarity: Human, Mouse. Host: Goat. More details

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Sample Manual Insert View PDF
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Testing Data View PDF
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Technical Datasheet View PDF
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Product Overview

Product Name
WFDC1/PS20, Polyclonal Antibody
Full Product Name
Goat anti-WFDC1/PS20 Antibody
Product Gene Name
Product Synonym Gene Name
PS20
Product Synonym Names
WFDC1; WAP four-disulfide core domain 1; PS20; WAP four-disulfide core domain 1 homolog (mouse); prostate stromal protein ps20; WFDC1 antibody; WAP four-disulfide core domain 1 antibody; PS20 antibody; WAP four-disulfide core domain 1 homolog (mouse) antibody; prostate stromal protein ps20 antibody; WFDC1 / PS20
Immunogen Sequence
KNVAEPGRGQ QKHFQ
Clonality
Polyclonal
Host
Goat
Species Reactivity
Expected from sequence similarity: Human, Mouse
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
100ug specific antibody in 200ul (lot specific)
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Molecular Weight
23,977 Da
OMIM
605322
Immunogen
Peptide with sequence C-KNVAEPGRGQQKHFQ, from the C Terminus of the protein sequence according to NP_067020.2.
Epitope
C Terminus
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Applications Tested/Suitable for anti-WFDC1 antibody
Peptide ELISA (EIA); Flow Cytometry (FC/FACS)
Application Notes for anti-WFDC1 antibody
Peptide ELISA: antibody detection limit dilution 1:32000.
Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.

Flow Cytometry (FC/FACS) of anti-WFDC1 antibody

Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Goat Anti-WFDC1 Polyclonal Antibody – Cat# MBS420096

Other Notes

Small volumes of anti-WFDC1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for WFDC1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
WAP four-disulfide core domain protein 1 isoform 1
NCBI Official Synonym Full Names
WAP four-disulfide core domain 1
NCBI Official Symbol
WFDC1
NCBI Official Synonym Symbols
PS20
UniProt Protein Name
WAP four-disulfide core domain protein 1
UniProt Synonym Protein Names
Prostate stromal protein ps20; ps20 growth inhibitor
UniProt Gene Name
WFDC1
UniProt Synonym Gene Names
PS20
UniProt Entry Name
WFDC1_HUMAN
NCBI Protein Information
WAP four-disulfide core domain protein 1

NCBI Summary for WFDC1

This gene encodes a member of the WAP-type four disulfide core domain family. The WAP-type four-disulfide core domain contains eight cysteines forming four disulfide bonds at the core of the protein, and functions as a protease inhibitor in many family members. This gene is mapped to chromosome 16q24, an area of frequent loss of heterozygosity in cancers, including prostate, breast and hepatocellular cancers and Wilms' tumor. This gene is downregulated in many cancer types and may be involved in the inhibition of cell proliferation. The encoded protein may also play a role in the susceptibility of certain CD4 memory T cells to human immunodeficiency virus infection. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013]
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UniProt Comments for WFDC1

WFDC1: Has growth inhibitory activity.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 16q24.3

Cellular Component: extracellular space

Molecular Function: serine-type endopeptidase inhibitor activity

Biological Process: negative regulation of cell growth; negative regulation of epithelial cell proliferation; negative regulation of inflammatory response; regulation of cell growth; response to drug; response to estradiol stimulus
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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