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HSD3B1 Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS420114

MBS420114 is an antibody targeting HSD3B1. Also known as Goat anti-HSD3B1 Antibody. Reactive in Tested: Human; Expected from sequence similarity: Human. Host: Goat. More details

$450 / 0.1 mg +1 FREE 8GB USB
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Testing Data View PDF
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Product Overview

Online Datasheet in PDF
Product Name
HSD3B1, Polyclonal Antibody
Full Product Name
Goat anti-HSD3B1 Antibody
Product Gene Name
anti-HSD3B1 antibody
Product Synonym Names
HSD3B1; hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1; HGNC: 5217; HSD3B; HSDB3; Hydroxy-delta-5-steroid dehydrogenase; 3 beta- and steroid hydroxy-delta-5-steroid dehydrogenase; 3 beta-and steroid delta-isomerase 1; HSD3B1 antibody; hydroxy-delta-5-steroid dehydrogenase; 3 beta- and steroid delta-isomerase 1 antibody; HGNC: 5217 antibody; HSD3B antibody; HSDB3 antibody; Hydroxy-delta-5-steroid dehydrogenase antibody; 3 beta- and steroid hydroxy-delta-5-steroid dehydrogenase antibody; 3 beta-and steroid delta-isomerase 1 antibody
Immunogen Sequence
DRHKETLKSK TQ
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human; Expected from sequence similarity: Human
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
100ug specific antibody in 200ul (lot specific)
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Antibody/Peptide Pairs
HSD3B1 peptide (MBS425353) is used for blocking the activity of HSD3B1 antibody (MBS420114)
Molecular Weight
42,252 Da
OMIM
109715
Immunogen
Peptide with sequence C-DRHKETLKSKTQ, from the C Terminus of the protein sequence according to NP_000853.1.
Epitope
C Terminus
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-30820
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Applications Tested/Suitable for anti-HSD3B1 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes for anti-HSD3B1 antibody
Peptide ELISA: Antibody detection limit dilution 1: 128000.
Western Blot: Approx 40kDa band observed in Human Placenta lysates (calculated MW of 42.3kDa according to NP_000853.1). In transfected HEK293 transiently expressing Human HSD3B1 a band of approx. 45kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0. 01-0.1ug/ml.

Western Blot (WB) of anti-HSD3B1 antibody

(0. 01ug/ml) staining of Human Placenta lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Goat Anti-HSD3B1 Polyclonal Antibody – Cat# MBS420114

Western Blot (WB) of anti-HSD3B1 antibody

HEK293 overexpressing Human HSD3B1 (RC204497) and probed (mock transfection in first lane), tested by Origene.
Goat Anti-HSD3B1 Polyclonal Antibody, Western Blot (WB) – Cat# MBS420114

Other Notes

Small volumes of anti-HSD3B1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for HSD3B1. It may not necessarily be applicable to this product.

NCBI GI #
4504507
NCBI GeneID
3283
NCBI Accession #
NP_000853.1
UniProt Secondary Accession #
Q14545; Q8IV65; A8K691
UniProt Related Accession #
P14060
GenBank Nucleotide #
NM_000862.2
NCBI Official Full Name
3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1
NCBI Official Synonym Full Names
hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1
NCBI Official Symbol
HSD3B1
NCBI Official Synonym Symbols
I; HSD3B; HSDB3; HSDB3A; SDR11E1; 3BETAHSD
UniProt Protein Name
3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1
UniProt Synonym Protein Names
3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type I; 3-beta-HSD I; Trophoblast antigen FDO161GIncluding the following 2 domains:3-beta-hydroxy-Delta(5)-steroid dehydrogenase (EC:1.1.1.145)Alternative name(s):3-beta-hydroxy-5-ene steroid dehydrogenase; Progesterone reductase
Protein Family
3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase
UniProt Gene Name
HSD3B1
UniProt Synonym Gene Names
3BH; HSDB3A; 3-beta-HSD I
UniProt Entry Name
3BHS1_HUMAN
NCBI Protein Information
3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1

UniProt Comments for HSD3B1

HSD3B1: 3-beta-HSD is a bifunctional enzyme, that catalyzes the oxidative conversion of Delta(5)-ene-3-beta-hydroxy steroid, and the oxidative conversion of ketosteroids. The 3-beta-HSD enzymatic system plays a crucial role in the biosynthesis of all classes of hormonal steroids. Efficiently catalyzes the transformation of pregnenolone to progesterone, 17-alpha-hydroxypregnenolone to 17- alpha-hydroxyprogesterone, DHEA to 4-androstenedione, dihydrotestosterone to 5-alpha-androstane-3 beta,17 beta-diol, dehydroepiandrosterone to androstenedione and 5-alpha-androstan-3 beta,17 beta-diol to 5-alpha-dihydrotestosterone. Belongs to the 3-beta-HSD family.

Protein type: Isomerase; Oxidoreductase; Membrane protein, integral; Lipid Metabolism - androgen and estrogen; Mitochondrial; EC 5.3.3.1; Lipid Metabolism - C21-steroid hormone; EC 1.1.1.145

Chromosomal Location of Human Ortholog: 1p13.1

Cellular Component: endoplasmic reticulum membrane; integral to membrane; mitochondrial inner membrane; mitochondrial intermembrane space; smooth endoplasmic reticulum membrane

Molecular Function: 3-beta-hydroxy-delta5-steroid dehydrogenase activity; steroid delta-isomerase activity

Biological Process: androgen biosynthetic process; estrogen biosynthetic process; glucocorticoid biosynthetic process; mineralocorticoid biosynthetic process; steroid biosynthetic process
Show more

Research Articles on HSD3B1

1. HSD3B1 is involved in aldosterone production, despite its lower levels of expression compared with HSD3B2, and also possibly associated with KCNJ5 mutation in aldosterone-producing adenoma.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-HSD3B1 antibody

 Products by Pathway  Pathway Diagram
 Aldosterone Synthesis And Secretion Pathway antibodies  Aldosterone Synthesis And Secretion Pathway Diagram
 Aldosterone Synthesis And Secretion Pathway antibodies  Aldosterone Synthesis And Secretion Pathway Diagram
 Androgen Biosynthesis Pathway antibodies  Androgen Biosynthesis Pathway Diagram
 C19/C18-Steroid Hormone Biosynthesis, Pregnenolone => Androstenedione => Estrone Pathway antibodies  C19/C18-Steroid Hormone Biosynthesis, Pregnenolone => Androstenedione => Estrone Pathway Diagram
 C19/C18-Steroid Hormone Biosynthesis, Pregnenolone => Androstenedione => Estrone Pathway antibodies  C19/C18-Steroid Hormone Biosynthesis, Pregnenolone => Androstenedione => Estrone Pathway Diagram
 Corticotropin-releasing Hormone Pathway antibodies  Corticotropin-releasing Hormone Pathway Diagram
 Glucocorticoid & Mineralcorticoid Metabolism Pathway antibodies  Glucocorticoid & Mineralcorticoid Metabolism Pathway Diagram
 Glucocorticoid Biosynthesis Pathway antibodies  Glucocorticoid Biosynthesis Pathway Diagram
 Metabolic Pathways antibodies  Metabolic Pathways Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram

Diseases associated with anti-HSD3B1 antibody

 Disease Name  Pubmed Publications
 Genital Diseases, Male Antibodies  >19 publications with HSD3B1 and Genital Diseases, Male
 Hyperplasia Antibodies  >15 publications with HSD3B1 and Hyperplasia
 Hypertension Antibodies  >13 publications with HSD3B1 and Hypertension
 Prostatic Neoplasms Antibodies  >13 publications with HSD3B1 and Prostatic Neoplasms
 Cardiovascular Diseases Antibodies  >11 publications with HSD3B1 and Cardiovascular Diseases
 Congenital Abnormalities Antibodies  >9 publications with HSD3B1 and Congenital Abnormalities
 Breast Neoplasms Antibodies  >8 publications with HSD3B1 and Breast Neoplasms
 Necrosis Antibodies  >6 publications with HSD3B1 and Necrosis
 Hypertrophy Antibodies  >3 publications with HSD3B1 and Hypertrophy
 Infertility, Male Antibodies  >3 publications with HSD3B1 and Infertility, Male

Organs/Tissues associated with anti-HSD3B1 antibody

 Organ/Tissue Name  Pubmed Publications
 Testis Antibodies  >44 publications with HSD3B1 and Testis
 Blood Antibodies  >38 publications with HSD3B1 and Blood
 Placenta Antibodies  >23 publications with HSD3B1 and Placenta
 Embryonic Tissue Antibodies  >11 publications with HSD3B1 and Embryonic Tissue
 Uterus Antibodies  >8 publications with HSD3B1 and Uterus
 Heart Antibodies  >5 publications with HSD3B1 and Heart
 Skin Antibodies  >3 publications with HSD3B1 and Skin
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