Goat Anti-OSBPL1A Polyclonal Antibody – Cat# MBS420151

Staining (0.5ug/ml) of Human Muscle lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

OSBPL1A Antibody – Goat Polyclonal

Name: OSBPL1A Antibody; Polyclonal; Goat; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS420151
Price: 450 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS420151

MBS420151 is an antibody targeting OSBPL1A. Also known as Goat anti-ORP1 Antibody. Reactive in Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow. Host: Goat. More details

$450 / 0.1 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

ORP1 (OSBPL1A), Polyclonal Antibody

Full Product Name

Goat anti-ORP1 Antibody

Product Gene Name

anti-OSBPL1A antibody

Product Synonym Gene Name

ORP1

Product Synonym Names

OSBPL1A; ORP1; OSBPL1B; FLJ10217; oxysterol binding protein-like 1A; OSBP-related protein 1; oxysterol-binding protein-like 1B; oxysterol-binding protein-related protein 1; ORP-1; oxysterol-binding protein-like 1A; OSBPL1A antibody; ORP1 antibody; OSBPL1B antibody; FLJ10217 antibody; oxysterol binding protein-like 1A antibody; OSBP-related protein 1 antibody; oxysterol-binding protein-like 1B antibody; oxysterol-binding protein-related protein 1 antibody; ORP-1 antibody; oxysterol-binding protein-like 1A antibody

Immunogen Sequence

YWDRNYFNLP DIY

Clonality

Polyclonal

Host

Goat

Species Reactivity

Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow

Form/Format

Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Purity/Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

100ug specific antibody in 200ul (lot specific)

Preparation and Storage

Aliquot and store at -20 degree C. Minimize freezing and thawing.

Antibody/Peptide Pairs

ORP1 peptide (MBS426109) is used for blocking the activity of ORP1 antibody (MBS420151)

Molecular Weight

65,095 Da

OMIM

606730

Immunogen

Peptide with sequence C-YWDRNYFNLPDIY, from the C Terminus of the protein sequence according to NP_060500.3; NP_542164.2.

Epitope

C Terminus

Note

This antibody is expected to recognise both reported human isoforms of this protein.

ISO Certification

Manufactured in an ISO 9001:2008 Certified Laboratory.

Applications Tested/Suitable for anti-OSBPL1A antibody

Peptide ELISA (EIA), Western Blot (WB)

Application Notes for anti-OSBPL1A antibody

Peptide ELISA: Antibody detection limit dilution 1: 16000.
Western Blot: Approx 48kDa band observed in Human Skeletal Muscle lysates (predicted MW of 50kDa according to NP_060500). Recommended for use at 0.5-2ug/ml.

Product References and Citations for anti-OSBPL1A antibody

Shah AH, Cianciola NL, Mills JL, Sönnichsen FD, Carlin C.; Adenovirus RIDalpha regulates endosome maturation by mimicking GTP-Rab7.; J Cell Biol.2007 Dec 3;179(5): 965-80.; Pubmed 18039930

Other Notes

Small volumes of anti-OSBPL1A antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for OSBPL1A. It may not necessarily be applicable to this product.

NCBI GI #

19718746

NCBI GeneID

114876

NCBI Accession #

NP_060500.3

NCBI Related Accession #

NP_542164.2

UniProt Secondary Accession #

Q9BZF5; Q9NW87; B7Z7D3

UniProt Related Accession #

Q9BXW6

GenBank Nucleotide #

NM_018030.4

NCBI Official Full Name

oxysterol-binding protein-related protein 1 isoform 1

NCBI Official Synonym Full Names

oxysterol binding protein like 1A

NCBI Official Symbol

OSBPL1A

NCBI Official Synonym Symbols

ORP1; ORP-1; OSBPL1B

UniProt Protein Name

Oxysterol-binding protein-related protein 1

Protein Family

Oxysterol-binding protein-related protein

UniProt Gene Name

OSBPL1A

UniProt Synonym Gene Names

ORP1; OSBP8; OSBPL1; OSBPL1B; ORP-1; OSBP-related protein 1

UniProt Entry Name

OSBL1_HUMAN

NCBI Protein Information

oxysterol-binding protein-related protein 1

NCBI Summary for OSBPL1A

This gene encodes a member of the oxysterol-binding protein (OSBP) family, a group of intracellular lipid receptors. Most members contain an N-terminal pleckstrin homology domain and a highly conserved C-terminal OSBP-like sterol-binding domain, although some members contain only the sterol-binding domain. Transcript variants derived from alternative promoter usage and/or alternative splicing exist; they encode different isoforms. [provided by RefSeq, Jul 2008]

UniProt Comments for OSBPL1A

OSBPL1A: Binds phospholipids; exhibits strong binding to phosphatidic acid and weak binding to phosphatidylinositol 3- phosphate. Stabilizes GTP-bound RAB7A on late endosomes/lysosomes and alters functional properties of late endocytic compartments via its interaction with RAB7A. Belongs to the OSBP family. 2 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 18q11.1

Cellular Component: cytoplasm; intracellular; late endosome; lysosomal membrane; nucleoplasm; nucleus

Molecular Function: cholesterol binding; phospholipid binding; protein binding

Biological Process: antigen processing and presentation of exogenous peptide antigen via MHC class II; cholesterol metabolic process; lipid transport; vesicle-mediated transport

Research Articles on OSBPL1A

1. Data suggest RIDalpha as a model system for understanding physiological egress routes that use ORP1L to activate endosome-to-endoplasmic reticulum (ER) feedback responses involved in lipid droplets (LDs)formation.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-OSBPL1A antibody

Products by Pathway	Pathway Diagram
Adaptive Immune System Pathway antibodies	Adaptive Immune System Pathway Diagram
Immune System Pathway antibodies	Immune System Pathway Diagram
MHC Class II Antigen Presentation Pathway antibodies	MHC Class II Antigen Presentation Pathway Diagram

Diseases associated with anti-OSBPL1A antibody

Disease Name	Pubmed Publications
Atherosclerosis Antibodies	>2 publications with OSBPL1A and Atherosclerosis
Lung Neoplasms Antibodies	>1 publications with OSBPL1A and Lung Neoplasms
Dyslipidemias Antibodies	>1 publications with OSBPL1A and Dyslipidemias
Disease Models, Animal Antibodies	>1 publications with OSBPL1A and Disease Models, Animal
Liver Neoplasms Antibodies	>1 publications with OSBPL1A and Liver Neoplasms

Organs/Tissues associated with anti-OSBPL1A antibody

Organ/Tissue Name	Pubmed Publications
Bone Antibodies	>2 publications with OSBPL1A and Bone
Liver Antibodies	>2 publications with OSBPL1A and Liver
Brain Antibodies	>2 publications with OSBPL1A and Brain
Placenta Antibodies	>1 publications with OSBPL1A and Placenta
Eye Antibodies	>1 publications with OSBPL1A and Eye
Prostate Antibodies	>1 publications with OSBPL1A and Prostate
Lung Antibodies	>1 publications with OSBPL1A and Lung
Embryonic Tissue Antibodies	>1 publications with OSBPL1A and Embryonic Tissue