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PCGF3 Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS420175

MBS420175 is an antibody targeting PCGF3. Also known as Goat anti-RNF3 (247 aa isoform) Antibody. Reactive in Tested: Human; Expected from sequence similarity: Human, Mouse, Dog, Cow. Host: Goat. More details

$450 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
RNF3 (PCGF3), Polyclonal Antibody
Full Product Name
Goat anti-RNF3 (247 aa isoform) Antibody
Product Gene Name
Product Synonym Gene Name
RNF3
Product Synonym Names
RNF3; DONG1; RNF3A; MGC40413; ring finger protein 3; PCGF3; polycomb group ring finger 3; DKFZp686D20235; FLJ36550; FLJ43813; MGC129615; RNF3 antibody; DONG1 antibody; RNF3A antibody; MGC40413 antibody; ring finger protein 3 antibody; PCGF3 antibody; polycomb group ring finger 3 antibody; DKFZp686D20235 antibody; FLJ36550 antibody; FLJ43813 antibody; MGC129615 antibody; RNF3 (247 aa isoform)
Immunogen Sequence
PLLLHYRPKM DLL
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Dog, Cow
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
100ug specific antibody in 200ul (lot specific)
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Antibody/Peptide Pairs
RNF3 peptide (MBS426072) is used for blocking the activity of RNF3 antibody (MBS420175)
Molecular Weight
28,748 Da
Immunogen
Peptide with sequence C-PLLLHYRPKMDLL, from the C Terminus of the protein sequence according to NP_006306.2.
Epitope
C Terminus
Note
This antibody is expected to recognise the 247 aa isoform only (NP_006306), not the 132 aa isoform (NP_694982).
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Applications Tested/Suitable for anti-PCGF3 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes for anti-PCGF3 antibody
Peptide ELISA: Antibody detection limit dilution 1: 2000.
Western Blot: Approx 35kDa band seen in Human Heart lysate [Predicted MW of approx.27kDa according to NP_006306]. Recommended for use at 0.5-2ug/ml.

Western Blot (WB) of anti-PCGF3 antibody

Staining (1ug/ml) of Human Heart lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Goat Anti-PCGF3 Polyclonal Antibody – Cat# MBS420175

Other Notes

Small volumes of anti-PCGF3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for PCGF3. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
polycomb group RING finger protein 3
NCBI Official Synonym Full Names
polycomb group ring finger 3
NCBI Official Symbol
PCGF3
NCBI Official Synonym Symbols
RNF3; DONG1; RNF3A
UniProt Protein Name
Polycomb group RING finger protein 3
UniProt Synonym Protein Names
RING finger protein 3A
UniProt Gene Name
PCGF3
UniProt Synonym Gene Names
RNF3; RNF3A
UniProt Entry Name
PCGF3_HUMAN
NCBI Protein Information
polycomb group RING finger protein 3

NCBI Summary for PCGF3

The protein encoded by this gene contains a C3HC4 type RING finger, which is a motif known to be involved in protein-protein interactions. The specific function of this protein has not yet been determined. [provided by RefSeq, Jul 2008]

UniProt Comments for PCGF3

PCGF3: Component of a Polycomb group (PcG) multiprotein PRC1- like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 4p16.3

Cellular Component: nucleus; PcG protein complex

Molecular Function: protein binding; zinc ion binding

Biological Process: regulation of transcription, DNA-dependent; transcription, DNA-dependent
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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