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ELMO1 Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS420226

MBS420226 is an antibody targeting ELMO1. Also known as Goat anti-ELMO1 Antibody. Reactive in Tested: Human, Mouse, Rat, Cow, Zebrafish; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow, Zebrafish. Host: Goat. More details

$450 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
ELMO1, Polyclonal Antibody
Full Product Name
Goat anti-ELMO1 Antibody
Product Gene Name
anti-ELMO1 antibody
Product Synonym Names
ELMO1; engulfment and cell motility 1 (ced-12 homolog, C. elegans); CED12; KIAA0281; ced-12 homolog 1; engulfment and cell motility 1; CED-12; ELMO-1; MGC126406; OTTHUMP00000207056; OTTHUMP00000207060; ELMO1 antibody; engulfment and cell motility 1 (ced-12 homolog; C. elegans) antibody; CED12 antibody; KIAA0281 antibody; ced-12 homolog 1 antibody; engulfment and cell motility 1 antibody; CED-12 antibody; ELMO-1 antibody; MGC126406 antibody; OTTHUMP00000207056 antibody; OTTHUMP00000207060 antibody
Immunogen Sequence
PKEPSNYDFV YDCN
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human, Mouse, Rat, Cow, Zebrafish; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow, Zebrafish
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
100ug specific antibody in 200ul (lot specific)
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Antibody/Peptide Pairs
ELMO1 peptide (MBS426235) is used for blocking the activity of ELMO1 antibody (MBS420226)
Molecular Weight
49,915 Da
OMIM
606420
Immunogen
Peptide with sequence PKEPSNYDFVYDCN, from the C Terminus of the protein sequence according to NP_055615.8; NP_569709.1; NP_001034548.1.
Epitope
C Terminus
Note
This antibody is expected to recognise both human isoforms of this protein. NP_569709.1 and NP_001034548.1 represent the same isoform.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Applications Tested/Suitable for anti-ELMO1 antibody
Peptide ELISA (EIA), Western Blot (WB), Immunoprecipitation (IP)
Application Notes for anti-ELMO1 antibody
Peptide ELISA: Antibody detection limit dilution 1: 128000.
Western Blot: Approx 83kDa band observed in Human Brain (Frontal Cortex) lysates, in Mouse and Rat Brain lysates and in lysates of cell line Jurkat (calculated MW of 83.8kDa according to NP_055615.8). Recommended concentration: 0.1-0.3ug/ml.
Immunoprecipitation: This antibody has been successfully used in IP from BAEC cells as described in the following paper: Epting et al, Circ Res.2010 Jul 9;107(1): 45-55; PMID: 20466982. IP; An anonymous customer found positive results in IP on Human promyelocytic leukemia cells (HL60).

Western Blot (WB) of anti-ELMO1 antibody

(0.3ug/ml) staining of Human Frontal Cortex (A), Mouse (B) and Rat (C) Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Goat Anti-ELMO1 Polyclonal Antibody – Cat# MBS420226

Western Blot (WB) of anti-ELMO1 antibody

(0.3ug/ml) staining of Jurkat cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Goat Anti-ELMO1 Polyclonal Antibody, Western Blot (WB) – Cat# MBS420226

Other Notes

Small volumes of anti-ELMO1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for ELMO1. It may not necessarily be applicable to this product.

NCBI GI #
18765700
NCBI GeneID
9844
NCBI Accession #
NP_055615.8
NCBI Related Accession #
NP_569709.1; NP_001034548.1
UniProt Secondary Accession #
Q29R79; Q6ZTJ0; Q96PB0; Q9H0I1; A4D1X6
UniProt Related Accession #
Q92556
GenBank Nucleotide #
NM_014800.10
NCBI Official Full Name
engulfment and cell motility protein 1 isoform 1
NCBI Official Synonym Full Names
engulfment and cell motility 1
NCBI Official Symbol
ELMO1
NCBI Official Synonym Symbols
CED12; CED-12; ELMO-1
UniProt Protein Name
Engulfment and cell motility protein 1
UniProt Synonym Protein Names
Protein ced-12 homolog
Protein Family
Engulfment and cell motility protein
UniProt Gene Name
ELMO1
UniProt Synonym Gene Names
KIAA0281
UniProt Entry Name
ELMO1_HUMAN
NCBI Protein Information
engulfment and cell motility protein 1

NCBI Summary for ELMO1

This gene encodes a member of the engulfment and cell motility protein family. These proteins interact with dedicator of cytokinesis proteins to promote phagocytosis and cell migration. Increased expression of this gene and dedicator of cytokinesis 1 may promote glioma cell invasion, and single nucleotide polymorphisms in this gene may be associated with diabetic nephropathy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013]
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UniProt Comments for ELMO1

ELMO1: Involved in cytoskeletal rearrangements required for phagocytosis of apoptotic cells and cell motility. Acts in assocation with DOCK1 and CRK. Was initially proposed to be required in complex with DOCK1 to activate Rac Rho small GTPases. May enhance the guanine nucleotide exchange factor (GEF) activity of DOCK1. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytoskeletal; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 7p14.1

Cellular Component: cytoplasm; cytosol; membrane; plasma membrane

Molecular Function: guanyl-nucleotide exchange factor activity; protein binding; SH3 domain binding

Biological Process: actin cytoskeleton organization and biogenesis; apoptosis; cell migration; cell motility; phagocytosis, engulfment; positive regulation of GTPase activity; Rac protein signal transduction; regulation of defense response to virus by virus; vascular endothelial growth factor receptor signaling pathway
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Research Articles on ELMO1

1. ELMO1 is expressed in rheumatoid arthritis synovium, promotes cell migration and invasion, and regulates Rac1 activity, thereby mediating rheumatoid arthritis pathogenicity.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-ELMO1 antibody

 Reference Product  PubMed Publications
 RAC1 antibody  >45 publications with ELMO1 and RAC1
 DOCK1 antibody  >36 publications with ELMO1 and DOCK1
 RHOG antibody  >13 publications with ELMO1 and RHOG
 SRC antibody  >11 publications with ELMO1 and SRC
 CRK antibody  >10 publications with ELMO1 and CRK
 DOCK2 antibody  >9 publications with ELMO1 and DOCK2
 HCK antibody  >5 publications with ELMO1 and HCK
 RAC2 antibody  >3 publications with ELMO1 and RAC2
 VAV1 antibody  >2 publications with ELMO1 and VAV1

Pathways associated with anti-ELMO1 antibody

 Products by Pathway  Pathway Diagram
 Bacterial Invasion Of Epithelial Cells Pathway antibodies  Bacterial Invasion Of Epithelial Cells Pathway Diagram
 Bacterial Invasion Of Epithelial Cells Pathway antibodies  Bacterial Invasion Of Epithelial Cells Pathway Diagram
 Chemokine Signaling Pathway antibodies  Chemokine Signaling Pathway Diagram
 Chemokine Signaling Pathway antibodies  Chemokine Signaling Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 Fcgamma Receptor (FCGR) Dependent Phagocytosis Pathway antibodies  Fcgamma Receptor (FCGR) Dependent Phagocytosis Pathway Diagram
 HIV Infection Pathway antibodies  HIV Infection Pathway Diagram
 Host Interactions Of HIV Factors Pathway antibodies  Host Interactions Of HIV Factors Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 Infectious Disease Pathway antibodies  Infectious Disease Pathway Diagram

Diseases associated with anti-ELMO1 antibody

 Disease Name  Pubmed Publications
 Kidney Diseases Antibodies  >19 publications with ELMO1 and Kidney Diseases
 Diabetic Nephropathies Antibodies  >16 publications with ELMO1 and Diabetic Nephropathies
 Renal Insufficiency Antibodies  >5 publications with ELMO1 and Renal Insufficiency
 Proteinuria Antibodies  >5 publications with ELMO1 and Proteinuria
 Inflammation Antibodies  >4 publications with ELMO1 and Inflammation
 Nervous System Diseases Antibodies  >2 publications with ELMO1 and Nervous System Diseases
 Brain Diseases Antibodies  >2 publications with ELMO1 and Brain Diseases
 Fibrosis Antibodies  >2 publications with ELMO1 and Fibrosis
 Vascular Diseases Antibodies  >2 publications with ELMO1 and Vascular Diseases
 Breast Neoplasms Antibodies  >2 publications with ELMO1 and Breast Neoplasms

Organs/Tissues associated with anti-ELMO1 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >14 publications with ELMO1 and Blood
 Kidney Antibodies  >14 publications with ELMO1 and Kidney
 Brain Antibodies  >14 publications with ELMO1 and Brain
 Lung Antibodies  >3 publications with ELMO1 and Lung
 Testis Antibodies  >3 publications with ELMO1 and Testis
 Muscle Antibodies  >3 publications with ELMO1 and Muscle
 Thymus Antibodies  >2 publications with ELMO1 and Thymus
 Thyroid Antibodies  >2 publications with ELMO1 and Thyroid
 Lymph Antibodies  >2 publications with ELMO1 and Lymph
 Lymph Node Antibodies  >2 publications with ELMO1 and Lymph Node
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