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RNF36/TRIM69 (TRIM69) Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS420264

MBS420264 is an antibody targeting TRIM69. Also known as Goat anti-RNF36/TRIM69 Antibody. Reactive in Tested: Human, Mouse; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow. Host: Goat. More details

$450 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
RNF36/TRIM69, Polyclonal Antibody
Full Product Name
Goat anti-RNF36/TRIM69 Antibody
Product Gene Name
Product Synonym Gene Name
RNF36
Product Synonym Names
TRIM69; tripartite motif-containing 69; HSD34; RNF36; Trif; ring finger protein 36; trimless; tripartite motif protein 69; hCG_39321; RNF36; TRIM69 antibody; tripartite motif-containing 69 antibody; HSD34 antibody; RNF36 antibody; Trif antibody; ring finger protein 36 antibody; trimless antibody; tripartite motif protein 69 antibody; hCG_39321 antibody; RNF36 antibody; RNF36 / TRIM69
Immunogen Sequence
GENKEPLHIL HPQ
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human, Mouse; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
100ug specific antibody in 200ul (lot specific)
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Antibody/Peptide Pairs
Molecular Weight
32,068 Da
OMIM
616017
Immunogen
Peptide with sequence C-GENKEPLHILHPQ, from the C Terminus of the protein sequence according to NP_892030.3; NP_542783.2.
Epitope
C Terminus
Note
This antibody is expected to recognize both reported isoforms (NP_892030.3; NP_542783.2).
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Applications Tested/Suitable for anti-TRIM69 antibody
Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-TRIM69 antibody
Peptide ELISA: Antibody detection limit dilution 1: 32000.
Immunohistochemistry: In paraffin embedded Human Testis shows speckled staining in nuclei of spermatocytes and spermitids. Recommended concentration, 2.5-3.8ug/ml.
Western Blot: Approx 55kDa band observed in Mouse Testis lysates (calculated MW of 57.3kDa according to Mouse NP_536771.2). Recommended concentration: 0.5-2ug/ml.

Western Blot (WB) of anti-TRIM69 antibody

(0.5ug/ml) staining of Mouse Testis lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Goat Anti-TRIM69 Polyclonal Antibody – Cat# MBS420264

Immunohistochemistry (IHC) of anti-TRIM69 antibody

(3.8ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Goat Anti-TRIM69 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS420264

Other Notes

Small volumes of anti-TRIM69 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for TRIM69. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI Related Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
E3 ubiquitin-protein ligase TRIM69 isoform a
NCBI Official Synonym Full Names
tripartite motif containing 69
NCBI Official Symbol
TRIM69
NCBI Official Synonym Symbols
Trif; HSD34; RNF36; HSD-34
UniProt Protein Name
E3 ubiquitin-protein ligase TRIM69
UniProt Synonym Protein Names
RFP-like domain-containing protein trimless; RING finger protein 36; Tripartite motif-containing protein 69
UniProt Gene Name
TRIM69
UniProt Synonym Gene Names
RNF36
UniProt Entry Name
TRI69_HUMAN
NCBI Protein Information
E3 ubiquitin-protein ligase TRIM69

NCBI Summary for TRIM69

This gene encodes a member of the RING-B-box-coiled-coil (RBCC) family and encodes a protein with an N-terminal RING finger motif, a PRY domain and a C-terminal SPRY domain. The mouse ortholog of this gene is specifically expressed in germ cells at the round spermatid stages during spermatogenesis and, when overexpressed, induces apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2008]
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UniProt Comments for TRIM69

TRIM69: May play a role in apoptosis. Belongs to the TRIM/RBCC family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 15q21.1

Cellular Component: cytoplasm; cytosol; nuclear speck; nucleus; plasma membrane

Molecular Function: ligase activity; protein binding; ubiquitin-protein ligase activity; zinc ion binding

Biological Process: apoptosis; protein polyubiquitination
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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