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PPID Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS420271

MBS420271 is an antibody targeting PPID. Also known as Goat anti-PPID/CyP-40 Antibody. Reactive in Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog. Host: Goat. More details

$450 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
PPID/CyP-40, Polyclonal Antibody
Full Product Name
Goat anti-PPID/CyP-40 Antibody
Product Gene Name
anti-PPID antibody
Product Synonym Gene Name
CyP-40
Product Synonym Names
CYP-40; MGC33096; peptidylprolyl isomerase D (cyclophilin D); 40 kDa peptidyl-prolyl cis-trans isomerase D; PPIase; cyclophilin 40; cyclophilin D; cyclophilin-related protein; peptidylprolyl isomerase D; rotamase; CYP-40 antibody; MGC33096 antibody; peptidylprolyl isomerase D (cyclophilin D) antibody; 40 kDa peptidyl-prolyl cis-trans isomerase D antibody; PPIase antibody; cyclophilin 40 antibody; cyclophilin D antibody; cyclophilin-related protein antibody; peptidylprolyl isomerase D antibody; rotamase antibody; PPID / CyP-40
Immunogen Sequence
DENFHYKHDR EG
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
100ug specific antibody in 200ul (lot specific)
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Antibody/Peptide Pairs
PPID / CyP-40 peptide (MBS426305) is used for blocking the activity of PPID/CyP-40 antibody (MBS420271)
Molecular Weight
40,764 Da
OMIM
601753
Immunogen
Peptide with sequence C-DENFHYKHDREG, from the internal region of the protein sequence according to NP_005029.1.
Epitope
Internal region
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Applications Tested/Suitable for anti-PPID antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes for anti-PPID antibody
Peptide ELISA: Antibody detection limit dilution 1: 64000.
Western Blot: Approx 40kDa band observed in Human Brain (Frontal Cortex) lysates (calculated MW of 40.8kDa according to NP_005029.1). In transfected HEK293 transiently expressing CyP-40 a band of approx. 45kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.1-0.3ug/ml.

Western Blot (WB) of anti-PPID antibody

(0.1ug/ml) staining of Human Brain (Frontal Cortex) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Goat Anti-PPID Polyclonal Antibody – Cat# MBS420271

Western Blot (WB) of anti-PPID antibody

HEK293 overexpressing CyP-40 (RC206039) and probed (mock transfection in first lane), tested by Origene.
Goat Anti-PPID Polyclonal Antibody, Western Blot (WB) – Cat# MBS420271

Other Notes

Small volumes of anti-PPID antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for PPID. It may not necessarily be applicable to this product.

NCBI GI #
4826932
NCBI GeneID
5481
NCBI Accession #
NP_005029.1
UniProt Secondary Accession #
B2R9V2
UniProt Related Accession #
Q08752
GenBank Nucleotide #
NM_005038.2
NCBI Official Full Name
peptidyl-prolyl cis-trans isomerase D
NCBI Official Synonym Full Names
peptidylprolyl isomerase D
NCBI Official Symbol
PPID
NCBI Official Synonym Symbols
CYPD; CYP-40
UniProt Protein Name
Peptidyl-prolyl cis-trans isomerase D
UniProt Synonym Protein Names
40 kDa peptidyl-prolyl cis-trans isomerase; Cyclophilin-40; CYP-40; Cyclophilin-related protein; Rotamase D
Protein Family
Peptidyl-prolyl cis-trans isomerase
UniProt Gene Name
PPID
UniProt Synonym Gene Names
CYP40; CYPD; PPIase D; CYP-40
UniProt Entry Name
PPID_HUMAN
NCBI Protein Information
peptidyl-prolyl cis-trans isomerase D

NCBI Summary for PPID

The protein encoded by this gene is a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. This protein has been shown to possess PPIase activity and, similar to other family members, can bind to the immunosuppressant cyclosporin A. [provided by RefSeq, Jul 2008]
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UniProt Comments for PPID

PPID: PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. Belongs to the cyclophilin-type PPIase family. PPIase D subfamily.

Protein type: EC 5.2.1.8; Nucleolus; Isomerase

Chromosomal Location of Human Ortholog: 4q31.3

Cellular Component: cytoplasm; nucleolus; nucleoplasm; nucleus

Molecular Function: cyclosporin A binding; estrogen receptor binding; heat shock protein binding; Hsp70 protein binding; Hsp90 protein binding; peptidyl-prolyl cis-trans isomerase activity; protein binding; transcription factor binding

Biological Process: apoptosis; negative regulation of transcription from RNA polymerase II promoter; positive regulation of apoptosis; positive regulation of protein secretion; positive regulation of viral genome replication; protein complex assembly; protein folding; protein peptidyl-prolyl isomerization; protein transport; release of virus from host
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Research Articles on PPID

1. cyclophilin-D protein could increase oxidative stress and cause endothelial cell injury and apoptosis. cyclophilin-D protein is the key factor in reactive oxygen species-induced mitochondrial damage, leading to apoptosis of endothelial cells.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-PPID antibody

 Reference Product  PubMed Publications
 STIP1 antibody  >1 publications with PPID and STIP1
 AHSA1 antibody  >1 publications with PPID and AHSA1

Pathways associated with anti-PPID antibody

 Products by Pathway  Pathway Diagram
 C-MYB Transcription Factor Network Pathway antibodies  C-MYB Transcription Factor Network Pathway Diagram

Diseases associated with anti-PPID antibody

 Disease Name  Pubmed Publications
 Necrosis Antibodies  >8 publications with PPID and Necrosis
 Hyperplasia Antibodies  >6 publications with PPID and Hyperplasia
 Cardiovascular Diseases Antibodies  >5 publications with PPID and Cardiovascular Diseases
 Inflammation Antibodies  >4 publications with PPID and Inflammation
 Weight Loss Antibodies  >4 publications with PPID and Weight Loss
 Vascular Diseases Antibodies  >4 publications with PPID and Vascular Diseases
 Hemorrhage Antibodies  >3 publications with PPID and Hemorrhage
 Liver Diseases Antibodies  >2 publications with PPID and Liver Diseases
 Fibrosis Antibodies  >2 publications with PPID and Fibrosis
 Hypertrophy Antibodies  >2 publications with PPID and Hypertrophy

Organs/Tissues associated with anti-PPID antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >44 publications with PPID and Blood
 Brain Antibodies  >42 publications with PPID and Brain
 Heart Antibodies  >5 publications with PPID and Heart
 Liver Antibodies  >4 publications with PPID and Liver
 Prostate Antibodies  >3 publications with PPID and Prostate
 Vascular Antibodies  >2 publications with PPID and Vascular
 Lung Antibodies  >2 publications with PPID and Lung
 Embryonic Tissue Antibodies  >2 publications with PPID and Embryonic Tissue
 Eye Antibodies  >2 publications with PPID and Eye
 Thyroid Antibodies  >1 publications with PPID and Thyroid
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