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Phosphodiesterase 4b (PDE4B) Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS420285

MBS420285 is an antibody targeting PDE4B. Also known as Goat anti-Phosphodiesterase 4B Antibody. Reactive in Tested: Mouse; Expected from sequence similarity: Human, Mouse, Rat. Host: Goat. More details

$450 / 0.1 mg +1 FREE 8GB USB
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Sample Manual Insert View PDF
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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
Phosphodiesterase 4B (PDE4B), Polyclonal Antibody
Full Product Name
Goat anti-Phosphodiesterase 4B Antibody
Product Gene Name
Product Synonym Names
Goat Anti-PDE4B Antibody; PDE4B; phosphodiesterase 4B, cAMP-specific (phosphodiesterase E4 dunce homolog, Drosophila); RP5-876C12.1; DKFZp686F2182; DPDE4; MGC126529; PDEIVB; OTTHUMP00000046896; cAMP-specific 3',5'-cyclic phosphodiesterase 4B; dunce-like phosphodiesterase E4; phos; PDE4B antibody; phosphodiesterase 4B; cAMP-specific (phosphodiesterase E4 dunce homolog; Drosophila) antibody; RP5-876C12.1 antibody; DKFZp686F2182 antibody; DPDE4 antibody; MGC126529 antibody; PDEIVB antibody; OTTHUMP00000046896 antibody; cAMP-specific 3\1; 5\1-cyclic phosphodiesterase 4B antibody; dunce-like phosphodiesterase E4 antibody; phosphodiesterase 4B; cAMP-specific antibody; phosphodiesterase 4B; cAMP-specific (dunce (Drosophila)-homolog phosphodiesterase E4) antibody; Phosphodiesterase 4B; Phosphodiesterase 4B
Immunogen Sequence
DIDIATEDKS PVDT
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Mouse; Expected from sequence similarity: Human, Mouse, Rat
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
100ug specific antibody in 200ul (lot specific)
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Antibody/Peptide Pairs
Molecular Weight
57,709 Da
OMIM
600127
Immunogen
Peptide with sequence C-DIDIATEDKSPVDT, from the C Terminus of the protein sequence according to NP_002591.2; NP_001032418.1; NP_001032416.1; NP_001032417.1.
Epitope
C Terminus
Note
This antibody is expected to recognise all reported isoforms (NP_002591.2, NP_001032418.1, NP_001032416.1 and NP_001032417.1).
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Applications Tested/Suitable for anti-PDE4B antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes for anti-PDE4B antibody
Peptide ELISA: Antibody detection limit dilution 1: 8000.
Western Blot: Approx 85kDa band observed in Mouse Brain lysates (calculated MW of 83.3kDa according to Human NP_002591.2 and 82.1kDa according to Mouse NP_062814.2). In transfected HEK293 transiently expressing Human PDE4B a band of approx.85kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.5-1.0 ug/ml.

Western Blot (WB) of anti-PDE4B antibody

(0.5ug/ml) staining of Mouse Brain Lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Goat Anti-PDE4B Polyclonal Antibody – Cat# MBS420285

Western Blot (WB) of anti-PDE4B antibody

HEK293 overexpressing Human PDE4B (RC211956) and probed (mock transfection in first lane), tested by Origene.
Goat Anti-PDE4B Polyclonal Antibody, Western Blot (WB) – Cat# MBS420285

Other Notes

Small volumes of anti-PDE4B antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for PDE4B. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI Related Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
cAMP-specific 3',5'-cyclic phosphodiesterase 4B isoform 1
NCBI Official Synonym Full Names
phosphodiesterase 4B
NCBI Official Symbol
PDE4B
NCBI Official Synonym Symbols
DPDE4; PDEIVB
UniProt Protein Name
cAMP-specific 3',5'-cyclic phosphodiesterase 4B
UniProt Synonym Protein Names
DPDE4; PDE32
UniProt Gene Name
PDE4B
UniProt Synonym Gene Names
DPDE4
UniProt Entry Name
PDE4B_HUMAN
NCBI Protein Information
cAMP-specific 3',5'-cyclic phosphodiesterase 4B

NCBI Summary for PDE4B

This gene is a member of the type IV, cyclic AMP (cAMP)-specific, cyclic nucleotide phosphodiesterase (PDE) family. The encoded protein regulates the cellular concentrations of cyclic nucleotides and thereby play a role in signal transduction. Altered activity of this protein has been associated with schizophrenia and bipolar affective disorder. Alternative splicing and the use of alternative promoters results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2014]
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UniProt Comments for PDE4B

PDE4B: a cAMP-specific phosphodiesterase. May be involved in mediating central nervous system effects of therapeutic agents ranging from antidepressants to antiasthmatic and anti-inflammatory agents. Inhibited by rolipram. Three alternatively-spliced isoforms have been described.

Protein type: EC 3.1.4.53; Nucleotide Metabolism - purine; Phosphodiesterase

Chromosomal Location of Human Ortholog: 1p31

Cellular Component: cytosol; voltage-gated calcium channel complex; Z disc

Molecular Function: 3',5'-cyclic-AMP phosphodiesterase activity; cAMP binding

Biological Process: cAMP catabolic process; leukocyte migration; neutrophil chemotaxis; neutrophil homeostasis; positive regulation of interferon-gamma production; positive regulation of interleukin-2 production; T cell receptor signaling pathway
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-PDE4B antibody

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