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MAD4/MXD4 (MXD4) Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS420332

MBS420332 is an antibody targeting MXD4. Also known as Goat anti-MAD4/MXD4 Antibody. Reactive in Tested: Human; Expected from sequence similarity: Human. Host: Goat. More details

$450 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
MAD4/MXD4, Polyclonal Antibody
Full Product Name
Goat anti-MAD4/MXD4 Antibody
Product Gene Name
Product Synonym Gene Name
MAD4
Product Synonym Names
MXD4; MAD4; MAX dimerization protein 4; Mad4 homolog; MST149; MSTP149; bHLHc12; MXD4 antibody; MAD4 antibody; MAX dimerization protein 4 antibody; Mad4 homolog antibody; MST149 antibody; MSTP149 antibody; bHLHc12 antibody; MAD4 / MXD4
Immunogen Sequence
GPHCRRLGRP ALS
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human; Expected from sequence similarity: Human
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
100ug specific antibody in 200ul (lot specific)
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Antibody/Peptide Pairs
Molecular Weight
23,528 Da
Immunogen
Peptide with sequence GPHCRRLGRPALS, from the C Terminus of the protein sequence according to NP_006445.1.
Epitope
C Terminus
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Applications Tested/Suitable for anti-MXD4 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes for anti-MXD4 antibody
Peptide ELISA: Antibody detection limit dilution 1: 16000.
Western Blot: Approx 22kDa band observed in human kidney lysates (predicted MW of 24kDa according to NP_006445). Recommended for use at 0.5-2ug/ml. Please note the appearance of this band was blocked by incubation with the immunising peptide.

Western Blot (WB) of anti-MXD4 antibody

Staining (0.5ug/ml) of human kidney lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Goat Anti-MXD4 Polyclonal Antibody – Cat# MBS420332

Other Notes

Small volumes of anti-MXD4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for MXD4. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
max dimerization protein 4
NCBI Official Synonym Full Names
MAX dimerization protein 4
NCBI Official Symbol
MXD4
NCBI Official Synonym Symbols
MAD4; MST149; MSTP149; bHLHc12
UniProt Protein Name
Max dimerization protein 4
UniProt Synonym Protein Names
Class C basic helix-loop-helix protein 12; bHLHc12; Max-associated protein 4; Max-interacting transcriptional repressor MAD4
UniProt Gene Name
MXD4
UniProt Synonym Gene Names
BHLHC12; MAD4; Max dimerizer 4; bHLHc12
UniProt Entry Name
MAD4_HUMAN
NCBI Protein Information
max dimerization protein 4

NCBI Summary for MXD4

This gene is a member of the MAD gene family . The MAD genes encode basic helix-loop-helix-leucine zipper proteins that heterodimerize with MAX protein, forming a transcriptional repression complex. The MAD proteins compete for MAX binding with MYC, which heterodimerizes with MAX forming a transcriptional activation complex. Studies in rodents suggest that the MAD genes are tumor suppressors and contribute to the regulation of cell growth in differentiating tissues. [provided by RefSeq, Jul 2008]
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UniProt Comments for MXD4

Mad4: Transcriptional repressor. Binds with MAX to form a sequence-specific DNA-binding protein complex which recognizes the core sequence 5'-CAC[GA]TG-3'. Antagonizes MYC transcriptional activity by competing for MAX and suppresses MYC dependent cell transformation.

Chromosomal Location of Human Ortholog: 4p16.3

Cellular Component: nucleus

Molecular Function: DNA binding; protein binding; protein dimerization activity; transcription corepressor activity

Biological Process: negative regulation of cell proliferation; negative regulation of transcription from RNA polymerase II promoter; transcription, DNA-dependent
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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