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VPS37C Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS420341

MBS420341 is an antibody targeting VPS37C. Also known as Goat anti-VPS37C Antibody. Reactive in Tested: Human; Expected from sequence similarity: Human. Host: Goat. More details

$450 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
VPS37C, Polyclonal Antibody
Full Product Name
Goat anti-VPS37C Antibody
Product Gene Name
Product Synonym Names
VPS37C; vacuolar protein sorting 37C (yeast); FLJ20847; vacuolar protein sorting 37C; vacuolar protein sorting 37 homolog C (S. cerevisiae); VPS37C antibody; vacuolar protein sorting 37C (yeast) antibody; FLJ20847 antibody; vacuolar protein sorting 37C antibody; vacuolar protein sorting 37 homolog C (S. cerevisiae) antibody
Immunogen Sequence
ETLKDKTLQE LEELQ
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human; Expected from sequence similarity: Human
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
100ug specific antibody in 200ul (lot specific)
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Antibody/Peptide Pairs
VPS37C peptide (MBS425233) is used for blocking the activity of VPS37C antibody (MBS420341)
Molecular Weight
38,659 Da
OMIM
610038
Immunogen
Peptide with sequence C-ETLKDKTLQELEELQ, from the internal region of the protein sequence according to NP_060436.4.
Epitope
Internal region
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Applications Tested/Suitable for anti-VPS37C antibody
Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-VPS37C antibody
Peptide ELISA: Antibody detection limit dilution 1: 32000.
Immunohistochemistry: In paraffin embedded Human Prostate shows strong cytoplasm staining in stromal cells. Recommended concentration, 3-6ug/ml.
Western Blot: Approx 38kDa band observed in human duodenum lysates (calculated MW of 38.7kDa according to NP_060436.4). Recommended concentration: 0. 03 - 0.1ug/ml.

Western Blot (WB) of anti-VPS37C antibody

(0. 03g/ml) staining of human duodenum lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Goat Anti-VPS37C Polyclonal Antibody – Cat# MBS420341

Immunohistochemistry (IHC) of anti-VPS37C antibody

(3.8ug/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Goat Anti-VPS37C Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS420341

Other Notes

Small volumes of anti-VPS37C antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for VPS37C. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
vacuolar protein sorting-associated protein 37C
NCBI Official Synonym Full Names
VPS37C, ESCRT-I subunit
NCBI Official Symbol
VPS37C
UniProt Protein Name
Vacuolar protein sorting-associated protein 37C
UniProt Synonym Protein Names
ESCRT-I complex subunit VPS37C
UniProt Gene Name
VPS37C
UniProt Synonym Gene Names
PML39; hVps37C
UniProt Entry Name
VP37C_HUMAN
NCBI Protein Information
vacuolar protein sorting-associated protein 37C

NCBI Summary for VPS37C

VPS37C is a subunit of ESCRT-I (endosomal sorting complex required for transport I), a complex in the class E vacuolar protein sorting (VPS) pathway required for sorting ubiquitinated transmembrane proteins into internal vesicles of multivesicular bodies (Eastman et al., 2005 [PubMed 15509564]).[supplied by OMIM, Mar 2008]
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UniProt Comments for VPS37C

VPS37C: Component of the ESCRT-I complex, a regulator of vesicular trafficking process. Required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies. May be involved in cell growth and differentiation. Belongs to the VPS37 family.

Chromosomal Location of Human Ortholog: 11q12.2

Cellular Component: endosome membrane; late endosome membrane

Molecular Function: calcium-dependent protein binding; protein binding

Biological Process: autophagy; endosome transport; protein transport; viral infectious cycle
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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