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GAD2 Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS420397

MBS420397 is an antibody targeting GAD2. Also known as Goat anti-GAD2/GAD65 Antibody. Reactive in Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog. Host: Goat. More details

$450 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
GAD2/GAD65, Polyclonal Antibody
Full Product Name
Goat anti-GAD2/GAD65 Antibody
Product Gene Name
anti-GAD2 antibody
Product Synonym Gene Name
GAD65
Product Synonym Names
GAD2; GAD65; glutamate decarboxylase 2 (pancreatic islets and brain, 65kDa); Glutamate decarboxylase-2 (pancreas); glutamate decarboxylase 2 (pancreatic islets and brain, 65kD); RP11-420F12.2; MGC161605; MGC161607; Glutamate decarboxylase-2 (pancreas); gl; GAD2 antibody; GAD65 antibody; glutamate decarboxylase 2 (pancreatic islets and brain; 65kDa) antibody; Glutamate decarboxylase-2 (pancreas) antibody; glutamate decarboxylase 2 (pancreatic islets and brain; 65kD) antibody; RP11-420F12.2 antibody; MGC161605 antibody; MGC161607 antibody; Glutamate decarboxylase-2 (pancreas) antibody; GAD2 / GAD65
Immunogen Sequence
TLEDNEERMS RLSK
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
100ug specific antibody in 200ul (lot specific)
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Antibody/Peptide Pairs
GAD2 / GAD65 peptide (MBS426951) is used for blocking the activity of GAD2/GAD65 antibody (MBS420397)
Molecular Weight
65,411 Da
OMIM
138275
Immunogen
Peptide with sequence C-TLEDNEERMSRLSK, from the internal region of the protein sequence according to NP_000809.1; NP_001127838.1.
Epitope
Internal region
Note
This antibody is expected to recognise both reported variants.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Applications Tested/Suitable for anti-GAD2 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes for anti-GAD2 antibody
Peptide ELISA: Antibody detection limit dilution 1: 16000.

Western Blot (WB) of anti-GAD2 antibody

(0.3ug/ml) staining of Human Hippocampus lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Goat Anti-GAD2 Polyclonal Antibody – Cat# MBS420397

Other Notes

Small volumes of anti-GAD2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for GAD2. It may not necessarily be applicable to this product.

NCBI GI #
4503875
NCBI GeneID
2572
NCBI Accession #
NP_000809.1
NCBI Related Accession #
NP_001127838.1
UniProt Secondary Accession #
Q9UD87
UniProt Related Accession #
Q05329
GenBank Nucleotide #
NM_000818.2
NCBI Official Full Name
glutamate decarboxylase 2
NCBI Official Synonym Full Names
glutamate decarboxylase 2
NCBI Official Symbol
GAD2
NCBI Official Synonym Symbols
GAD65
UniProt Protein Name
Glutamate decarboxylase 2
UniProt Synonym Protein Names
65 kDa glutamic acid decarboxylase; GAD-65; Glutamate decarboxylase 65 kDa isoform
Protein Family
Glutamate decarboxylase
UniProt Gene Name
GAD2
UniProt Synonym Gene Names
GAD65; GAD-65
UniProt Entry Name
DCE2_HUMAN
NCBI Protein Information
glutamate decarboxylase 2

NCBI Summary for GAD2

This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantibody and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Oct 2008]
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UniProt Comments for GAD2

GAD2: Catalyzes the production of GABA. Homodimer. Belongs to the group II decarboxylase family.

Protein type: Other Amino Acids Metabolism - taurine and hypotaurine; Lyase; EC 4.1.1.15; Amino Acid Metabolism - alanine, aspartate and glutamate; Carbohydrate Metabolism - butanoate; Other Amino Acids Metabolism - beta-alanine

Chromosomal Location of Human Ortholog: 10p11.23

Cellular Component: axon; cell junction; cytosol; Golgi membrane; perinuclear region of cytoplasm; plasma membrane; presynaptic membrane; synaptic vesicle membrane

Molecular Function: glutamate binding; glutamate decarboxylase activity; protein binding; protein heterodimerization activity; pyridoxal phosphate binding

Biological Process: glutamate decarboxylation to succinate; neurotransmitter biosynthetic process; neurotransmitter secretion; response to drug; synaptic transmission
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Research Articles on GAD2

1. we investigated the relationship between serum glutamic acid decarboxylase (GAD) autoantibody (Ab) levels and single nucleotide polymorphisms (SNPs) of the glutamic acid de-carboxylase 2 (GAD2) 5'-untranslated region

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-GAD2 antibody

 Products by Pathway  Pathway Diagram
 Alanine And Aspartate Metabolism Pathway antibodies  Alanine And Aspartate Metabolism Pathway Diagram
 Alanine, Aspartate And Glutamate Metabolism Pathway antibodies  Alanine, Aspartate And Glutamate Metabolism Pathway Diagram
 Alanine, Aspartate And Glutamate Metabolism Pathway antibodies  Alanine, Aspartate And Glutamate Metabolism Pathway Diagram
 Biogenic Amine Synthesis Pathway antibodies  Biogenic Amine Synthesis Pathway Diagram
 Butanoate Metabolism Pathway antibodies  Butanoate Metabolism Pathway Diagram
 Butanoate Metabolism Pathway antibodies  Butanoate Metabolism Pathway Diagram
 GABA (gamma-Aminobutyrate) Shunt Pathway antibodies  GABA (gamma-Aminobutyrate) Shunt Pathway Diagram
 GABA (gamma-Aminobutyrate) Shunt Pathway antibodies  GABA (gamma-Aminobutyrate) Shunt Pathway Diagram
 GABA Synthesis Pathway antibodies  GABA Synthesis Pathway Diagram
 GABA Synthesis, Release, Reuptake And Degradation Pathway antibodies  GABA Synthesis, Release, Reuptake And Degradation Pathway Diagram

Diseases associated with anti-GAD2 antibody

 Disease Name  Pubmed Publications
 Mental Disorders Antibodies  >24 publications with GAD2 and Mental Disorders
 Nervous System Diseases Antibodies  >14 publications with GAD2 and Nervous System Diseases
 Schizophrenia Antibodies  >12 publications with GAD2 and Schizophrenia
 Brain Diseases Antibodies  >11 publications with GAD2 and Brain Diseases
 Anxiety Disorders Antibodies  >6 publications with GAD2 and Anxiety Disorders
 Epilepsy Antibodies  >6 publications with GAD2 and Epilepsy
 Inflammation Antibodies  >6 publications with GAD2 and Inflammation
 Seizures Antibodies  >6 publications with GAD2 and Seizures
 Depressive Disorder Antibodies  >5 publications with GAD2 and Depressive Disorder
 Disease Models, Animal Antibodies  >4 publications with GAD2 and Disease Models, Animal

Organs/Tissues associated with anti-GAD2 antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >48 publications with GAD2 and Brain
 Pancreas Antibodies  >4 publications with GAD2 and Pancreas
 Uncharacterized Tissue Antibodies  >1 publications with GAD2 and Uncharacterized Tissue
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