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GPX3 Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS420423

MBS420423 is an antibody targeting GPX3. Also known as Goat anti-GPX3 Antibody. Reactive in Tested: Mouse, Pig; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig. Host: Goat. More details

$450 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
GPX3, Polyclonal Antibody
Full Product Name
Goat anti-GPX3 Antibody
Product Gene Name
Product Synonym Names
GPX3; glutathione peroxidase 3 (plasma); GPx-P; GSHPx-3; GSHPx-P; extracellular glutathione peroxidase; plasma glutathione peroxidase 3; GPX3 antibody; glutathione peroxidase 3 (plasma) antibody; GPx-P antibody; GSHPx-3 antibody; GSHPx-P antibody; extracellular glutathione peroxidase antibody; plasma glutathione peroxidase 3 antibody
Immunogen Sequence
NQFGKQEPGE NSE
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Mouse, Pig; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
100ug specific antibody in 200ul (lot specific)
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Antibody/Peptide Pairs
GPX3 peptide (MBS425753) is used for blocking the activity of GPX3 antibody (MBS420423)
Molecular Weight
25,552 Da
OMIM
138321
Immunogen
Peptide with sequence C-NQFGKQEPGENSE, from the internal region of the protein sequence according to NP_002075.2.
Epitope
Internal region
Note
This antibody may cross-react with epididymis-specific GPX5 and olfactory epithelium-speciific GPX6
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Applications Tested/Suitable for anti-GPX3 antibody
Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-GPX3 antibody
Peptide ELISA: Antibody detection limit dilution 1: 1000.
Immunohistochemistry: Frozen sections of Porcin Kidney shows vesicular staining of the cytoplasm in PCT epithelial cells. Recommended concentration, 20ug/ml.
Western Blot: Approx 26kDa band observed in Mouse Kidney and Liver lysates (calculated MW of 27.7kDa according to Mouse NP_001077398.1 [isoform1], 25.4kDa according to Mouse NP_032187.2 [isoform 2] and 25.5kDa according to Human NP_002075.2). Recommended concentration: 1-3ug/ml.

Western Blot (WB) of anti-GPX3 antibody

(1ug/ml) staining of Mouse Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Goat Anti-GPX3 Polyclonal Antibody – Cat# MBS420423

Immunofluorescence (IF) of anti-GPX3 antibody

(20ug/ml) staining of PFA-perfused cryosection of Porcin Kidney. Microwave antigen retrieval with citrate buffer pH 3, CY3-staining. Data obtained from Dr. Hrvoje Brzica, University of Zagreb, Croatia
Goat Anti-GPX3 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS420423

Other Notes

Small volumes of anti-GPX3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for GPX3. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
glutathione peroxidase 3
NCBI Official Synonym Full Names
glutathione peroxidase 3
NCBI Official Symbol
GPX3
NCBI Official Synonym Symbols
GPx-P; GSHPx-3; GSHPx-P
UniProt Protein Name
Glutathione peroxidase 3
UniProt Synonym Protein Names
Extracellular glutathione peroxidase; Plasma glutathione peroxidase; GPx-P; GSHPx-P
UniProt Gene Name
GPX3
UniProt Synonym Gene Names
GPXP; GPx-3; GSHPx-3; GPx-P; GSHPx-P
UniProt Entry Name
GPX3_HUMAN
NCBI Protein Information
glutathione peroxidase 3

NCBI Summary for GPX3

This gene product belongs to the glutathione peroxidase family, which functions in the detoxification of hydrogen peroxide. It contains a selenocysteine (Sec) residue at its active site. The selenocysteine is encoded by the UGA codon, which normally signals translation termination. The 3' UTR of Sec-containing genes have a common stem-loop structure, the sec insertion sequence (SECIS), which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. [provided by RefSeq, Jul 2008]
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UniProt Comments for GPX3

GPX3: Protects cells and enzymes from oxidative damage, by catalyzing the reduction of hydrogen peroxide, lipid peroxides and organic hydroperoxide, by glutathione. Belongs to the glutathione peroxidase family.

Protein type: EC 1.11.1.9; Oxidoreductase; Lipid Metabolism - arachidonic acid; Secreted; Other Amino Acids Metabolism - glutathione; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 5q33.1

Cellular Component: extracellular region; extracellular space

Molecular Function: glutathione peroxidase activity; protein binding; selenium binding; transcription factor binding

Biological Process: hydrogen peroxide catabolic process; protein homotetramerization; response to lipid hydroperoxide; response to reactive oxygen species
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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