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Swiprosin 1/EFHD2 (EFHD2) Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS420425

MBS420425 is an antibody targeting EFHD2. Also known as Goat anti-Swiprosin 1/EFHD2 Antibody. Reactive in Tested: Human, Mouse; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow. Host: Goat. More details

$450 / 0.1 mg +1 FREE 8GB USB
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Testing Data View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
Swiprosin 1/EFHD2, Polyclonal Antibody
Full Product Name
Goat anti-Swiprosin 1/EFHD2 Antibody
Product Gene Name
Product Synonym Names
EFHD2; MGC4342; EF hand domain containing 2; EF-hand domain family, member D2; RP3-467K16.3; SWS1; EF hand domain family, member D2; swiprosin 1; EFHD2 antibody; MGC4342 antibody; EF hand domain containing 2 antibody; Swiprosin 1; Swiprosin 1 / EFHD2
Immunogen Sequence
CAAFKELQST FK
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human, Mouse; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
100ug specific antibody in 200ul (lot specific)
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Antibody/Peptide Pairs
Molecular Weight
26,697 Da
OMIM
616450
Immunogen
Peptide with sequence CAAFKELQSTFK, from the C Terminus of the protein sequence according to NP_077305.2.
Epitope
C Terminus
Note
This antibody does not cross-react with Mouse Swiprosin 2. Data kindly provided by Sebastian Duetting and Dirk Mielenz.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Applications Tested/Suitable for anti-EFHD2 antibody
Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-EFHD2 antibody
Peptide ELISA: Antibody detection limit dilution 1: 128000.
Immunohistochemistry: In paraffin embedded Human Tonsil shows membranous staining in selected cells. Recommended concentration, 2-4ug/ml.
Western Blot: Approx 30kDa band observed in Human Tonsil, Human Thymus and Human Lymph Node and in Mouse Thymus and Mouse Lymph Node lysates (calculated MW of 26.7kDa according to NP_077305.2). Recommended concentration: 0.3-1. 0ug/ml.

Western Blot (WB) of anti-EFHD2 antibody

(0.1ug/ml) staining of Human Thymus (A), Lymph Node (B) and Tonsil (C) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Goat Anti-EFHD2 Polyclonal Antibody – Cat# MBS420425

Immunohistochemistry (IHC) of anti-EFHD2 antibody

(2ug/ml) staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.
Goat Anti-EFHD2 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS420425

Other Notes

Small volumes of anti-EFHD2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for EFHD2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
EF-hand domain-containing protein D2
NCBI Official Synonym Full Names
EF-hand domain family member D2
NCBI Official Symbol
EFHD2
NCBI Official Synonym Symbols
SWS1
UniProt Protein Name
EF-hand domain-containing protein D2
UniProt Synonym Protein Names
Swiprosin-1
UniProt Gene Name
EFHD2
UniProt Synonym Gene Names
SWS1
UniProt Entry Name
EFHD2_HUMAN
NCBI Protein Information
EF-hand domain-containing protein D2

UniProt Comments for EFHD2

EFHD2: May regulate B-cell receptor (BCR)-induced immature and primary B-cell apoptosis. Plays a role as negative regulator of the canonical NF-kappa-B-activating branch. Controls spontaneous apoptosis through the regulation of BCL2L1 abundance.

Protein type: Calcium-binding

Chromosomal Location of Human Ortholog: 1p36.21

Cellular Component: lipid raft

Molecular Function: calcium ion binding
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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