Goat Anti-C10ORF59 Polyclonal Antibody – Cat# MBS420455

(0.5ug/ml) staining of Rat Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

C10ORF59 Antibody – Goat Polyclonal

Name: C10ORF59 Antibody; Polyclonal; Goat; Mouse/Rat/Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS420455
Price: 450 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS420455

MBS420455 is an antibody targeting C10orf59. Also known as Goat anti-Renalase (aa 134 to 147) Antibody. Reactive in Tested: Mouse, Rat; Expected from sequence similarity: Human, Mouse, Rat. Host: Goat. More details

$450 / 0.1 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Renalase (C10orf59), Polyclonal Antibody

Full Product Name

Goat anti-Renalase (aa 134 to 147) Antibody

Product Gene Name

anti-C10orf59 antibody

Product Synonym Names

Goat Anti-Renalase (aa 224 to 233) Antibody; renalase; C10orf59; chromosome 10 open reading frame 59; FLJ11218; renalase antibody; C10orf59 antibody; chromosome 10 open reading frame 59 antibody; FLJ11218 antibody; Renalase; Renalase (aa 134 to 147)

Immunogen Sequence

QINLRDDKWE VSKQ

Clonality

Polyclonal

Host

Goat

Species Reactivity

Tested: Mouse, Rat; Expected from sequence similarity: Human, Mouse, Rat

Form/Format

Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Purity/Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

100ug specific antibody in 200ul (lot specific)

Preparation and Storage

Aliquot and store at -20 degree C. Minimize freezing and thawing.

Antibody/Peptide Pairs

Renalase peptide (MBS425400) is used for blocking the activity of Renalase antibody (MBS420455)

Molecular Weight

34,949 Da

OMIM

609360

Immunogen

Peptide with sequence C-QINLRDDKWEVSKQ, from the Internal region of the protein sequence according to NP_001026879.1; NP_060833.1.

Epitope

Internal region

Note

This antibody is expected to recognize both reported isoforms (NP_001026879.1 and NP_060833.1).

ISO Certification

Manufactured in an ISO 9001:2008 Certified Laboratory.

Applications Tested/Suitable for anti-C10orf59 antibody

Peptide ELISA (EIA), Western Blot (WB)

Application Notes for anti-C10orf59 antibody

Peptide ELISA: Antibody detection limit dilution 1: 32000.
Western Blot: Approx 37kDa band observed in Mouse Kidney and Rat Kidney lysates (calculated MW of 37.8kDa according to Human NP_001026879.1 and 35. 0kDa according to Rat NP_001014189.1). Recommended concentration: 0.5-1.5ug/ml.

Other Notes

Small volumes of anti-C10orf59 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for C10orf59. It may not necessarily be applicable to this product.

NCBI GI #

72534708

NCBI GeneID

55328

NCBI Accession #

NP_001026879.1

NCBI Related Accession #

NP_060833.1

UniProt Secondary Accession #

Q9BS33; Q9NUP8

UniProt Related Accession #

Q5VYX0

GenBank Nucleotide #

NM_001031709.1

NCBI Official Full Name

renalase isoform 1

NCBI Official Synonym Full Names

renalase, FAD-dependent amine oxidase

NCBI Official Symbol

RNLS

NCBI Official Synonym Symbols

C10orf59; RENALASE

UniProt Protein Name

Renalase

UniProt Synonym Protein Names

Monoamine oxidase-C; MAO-C

Protein Family

Renalase

UniProt Gene Name

RNLS

UniProt Synonym Gene Names

C10orf59; MAO-C

UniProt Entry Name

RNLS_HUMAN

NCBI Protein Information

renalase

NCBI Summary for C10orf59

Renalase is a flavin adenine dinucleotide-dependent amine oxidase that is secreted into the blood from the kidney (Xu et al., 2005 [PubMed 15841207]).[supplied by OMIM, Mar 2008]

UniProt Comments for C10orf59

RENALASE: Probable FAD-dependent amine oxidase secreted by the kidney, which circulates in blood and modulates cardiac function and systemic blood pressure. Degrades catecholamines such as dopamine, norepinephrine and epinephrine in vitro. Lowers blood pressure in vivo by decreasing cardiac contractility and heart rate and preventing a compensatory increase in peripheral vascular tone, suggesting a causal link to the increased plasma catecholamine and heightened cardiovascular risk. High concentrations of catecholamines activate plasma renalase and promotes its secretion and synthesis. According to PubMed:17385068, is unlikely that renalase has physiologically relevant catecholamine-oxidizing activity. Belongs to the renalase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Oxidoreductase; Secreted, signal peptide; Secreted; EC 1.6.3.5

Chromosomal Location of Human Ortholog: 10q23.31

Cellular Component: extracellular space

Molecular Function: oxidoreductase activity

Research Articles on C10orf59

1. the antihypertensive effect of renalase still remains a phenomenon with unclear biochemical mechanim(s) and functions of intracellular and extracellular (circulating) renalases obviously differ.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Diseases associated with anti-C10orf59 antibody

Disease Name	Pubmed Publications
Inflammation Antibodies	>3 publications with C10orf59 and Inflammation
Cardiovascular Diseases Antibodies	>2 publications with C10orf59 and Cardiovascular Diseases
Breast Neoplasms Antibodies	>2 publications with C10orf59 and Breast Neoplasms
Neoplasm Metastasis Antibodies	>2 publications with C10orf59 and Neoplasm Metastasis
Hypertension Antibodies	>2 publications with C10orf59 and Hypertension
Kidney Diseases Antibodies	>1 publications with C10orf59 and Kidney Diseases
Disease Models, Animal Antibodies	>1 publications with C10orf59 and Disease Models, Animal
Heart Diseases Antibodies	>1 publications with C10orf59 and Heart Diseases
Memory Disorders Antibodies	>1 publications with C10orf59 and Memory Disorders
Learning Disorders Antibodies	>1 publications with C10orf59 and Learning Disorders

Organs/Tissues associated with anti-C10orf59 antibody

Organ/Tissue Name	Pubmed Publications
Eye Antibodies	>2 publications with C10orf59 and Eye
Kidney Antibodies	>2 publications with C10orf59 and Kidney
Muscle Antibodies	>1 publications with C10orf59 and Muscle
Brain Antibodies	>1 publications with C10orf59 and Brain