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Proenkephalin (PENK) Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS420561

MBS420561 is an antibody targeting PENK. Also known as Goat anti-Proenkephalin Antibody. Reactive in Tested: Human; Expected from sequence similarity: Human. Host: Goat. More details

$450 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
Proenkephalin (PENK), Polyclonal Antibody
Full Product Name
Goat anti-Proenkephalin Antibody
Product Gene Name
anti-PENK antibody
Product Synonym Names
proenkephalin; PENK; proenkephalin antibody; PENK antibody; Proenkephalin; Proenkephalin
Immunogen Sequence
RSHHQDGSDN EE
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human; Expected from sequence similarity: Human
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
100ug specific antibody in 200ul (lot specific)
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Antibody/Peptide Pairs
Proenkephalin peptide (MBS426936) is used for blocking the activity of Proenkephalin antibody (MBS420561)
Molecular Weight
30.8 kDa
OMIM
131330
Immunogen
Peptide with sequence C-RSHHQDGSDNEE, from the internal region of the protein sequence according to NP_006202.1.
Epitope
Internal region
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Applications Tested/Suitable for anti-PENK antibody
Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-PENK antibody
Peptide ELISA: Antibody detection limit dilution 1: 32000.
Immunohistochemistry: Frozen section of Human Hypothalamus shows staining of dense enkephalinergic axon plexus and scattered neuronal cell bodies (higher magnification inset) in the human infundibular nucleus. Recommended concentration, 0.3-1ug/ml.
Western Blot: Approx 37kDa band observed in Human Adrenal Gland lysates (calculated MW of 30.8kDa according to NP_006202.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Normant and Loh, Endocrinology.1998 Apr;139(4): 2137-45; PMID: 9529003). Recommended concentration: 1-3ug/ml.

Western Blot (WB) of anti-PENK antibody

(0.3ug/ml) staining of Human Adrenal Gland lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Goat Anti-PENK Polyclonal Antibody – Cat# MBS420561

Immunofluorescence (IF) of anti-PENK antibody

(0.3ug/ml) staining of PFA-perfused cryosection of Human Hypothalamus. Antigen retrieval with citrate buffer pH 6 at 80C for 30min, Cy3-staining. Data obtained by Prof. Erik Hrabovszky, Inst, Exp, Med. , Budapest, Hungary.
Goat Anti-PENK Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS420561

Other Notes

Small volumes of anti-PENK antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for PENK. It may not necessarily be applicable to this product.

NCBI GI #
5453876
NCBI GeneID
5179
NCBI Accession #
NP_006202.1
UniProt Secondary Accession #
Q6FHC6; Q6FHE6; B2RC23
UniProt Related Accession #
P01210
GenBank Nucleotide #
NM_006211.3
NCBI Official Synonym Full Names
proenkephalin
NCBI Official Symbol
PENK
NCBI Official Synonym Symbols
PE; PENK-A
UniProt Protein Name
Proenkephalin-A
UniProt Synonym Protein Names
SynenkephalinMet-enkephalinAlternative name(s):Opioid growth factor; OGF
Protein Family
Proenkephalin
UniProt Gene Name
PENK
UniProt Synonym Gene Names
OGF
UniProt Entry Name
PENK_HUMAN
NCBI Protein Information
proenkephalin-A

NCBI Summary for PENK

This gene encodes a preproprotein that is proteolytically processed to generate multiple protein products. These products include the pentapeptide opioids Met-enkephalin and Leu-enkephalin, which are stored in synaptic vesicles, then released into the synapse where they bind to mu- and delta-opioid receptors to modulate the perception of pain. Other non-opioid cleavage products may function in distinct biological activities. [provided by RefSeq, Jul 2015]
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UniProt Comments for PENK

PENK: Met- and Leu-enkephalins compete with and mimic the effects of opiate drugs. They play a role in a number of physiologic functions, including pain perception and responses to stress. PENK(114-133) and PENK(237-258) increase glutamate release in the striatum. PENK(114-133) decreases GABA concentration in the striatum. Belongs to the opioid neuropeptide precursor family.

Protein type: Hormone; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 8q23-q24

Cellular Component: cell soma; dendrite; extracellular region; nerve terminal; perikaryon; plasma membrane

Molecular Function: neuropeptide hormone activity; opioid peptide activity; opioid receptor binding; protein binding

Biological Process: aging; behavioral fear response; general adaptation syndrome, behavioral process; glial cell proliferation; neuropeptide signaling pathway; osteoblast differentiation; response to calcium ion; response to estradiol stimulus; response to ethanol; response to hypoxia; response to lipopolysaccharide; response to morphine; response to nicotine; response to radiation; response to toxin; sensory perception; sensory perception of pain; signal transduction; startle response; synaptic transmission
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Research Articles on PENK

1. Serum PENK-A levels were not associated with all-cause mortality in patients with type 2 diabetes mellitus, and its association with cardiovascular mortality was strongly attenuated after accounting for all traditional risk factors.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-PENK antibody

 Products by Pathway  Pathway Diagram
 Class A/1 (Rhodopsin-like Receptors) Pathway antibodies  Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
 G Alpha (i) Signalling Events Pathway antibodies  G Alpha (i) Signalling Events Pathway Diagram
 GPCR Downstream Signaling Pathway antibodies  GPCR Downstream Signaling Pathway Diagram
 GPCR Ligand Binding Pathway antibodies  GPCR Ligand Binding Pathway Diagram
 Peptide Ligand-binding Receptors Pathway antibodies  Peptide Ligand-binding Receptors Pathway Diagram
 Signal Transduction Pathway antibodies  Signal Transduction Pathway Diagram
 Signaling By GPCR Pathway antibodies  Signaling By GPCR Pathway Diagram

Diseases associated with anti-PENK antibody

 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >39 publications with PENK and Nervous System Diseases
 Opioid-Related Disorders Antibodies  >33 publications with PENK and Opioid-Related Disorders
 Brain Diseases Antibodies  >26 publications with PENK and Brain Diseases
 Disease Models, Animal Antibodies  >19 publications with PENK and Disease Models, Animal
 Pain Antibodies  >18 publications with PENK and Pain
 Movement Disorders Antibodies  >13 publications with PENK and Movement Disorders
 Poisoning Antibodies  >9 publications with PENK and Poisoning
 Anxiety Disorders Antibodies  >9 publications with PENK and Anxiety Disorders
 Seizures Antibodies  >7 publications with PENK and Seizures
 Cocaine-Related Disorders Antibodies  >7 publications with PENK and Cocaine-Related Disorders

Organs/Tissues associated with anti-PENK antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >129 publications with PENK and Brain
 Muscle Antibodies  >11 publications with PENK and Muscle
 Bone Antibodies  >11 publications with PENK and Bone
 Pituitary Gland Antibodies  >6 publications with PENK and Pituitary Gland
 Eye Antibodies  >5 publications with PENK and Eye
 Prostate Antibodies  >5 publications with PENK and Prostate
 Lung Antibodies  >5 publications with PENK and Lung
 Ear Antibodies  >2 publications with PENK and Ear
 Skin Antibodies  >2 publications with PENK and Skin
 Ovary Antibodies  >2 publications with PENK and Ovary
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