Goat Anti-NAT10 Polyclonal Antibody – Cat# MBS420570

(3.8ug/ml) staining of paraffin embedded Human Colon. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

NAT10 Antibody – Goat Polyclonal

Name: NAT10 Antibody; Polyclonal; Goat; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS420570
Price: 450 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS420570

MBS420570 is an antibody targeting NAT10. Also known as Goat anti-NAT10/hALP Antibody. Reactive in Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog. Host: Goat. More details

$450 / 0.1 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

NAT10/hALP, Polyclonal Antibody

Full Product Name

Goat anti-NAT10/hALP Antibody

Product Gene Name

anti-NAT10 antibody

Product Synonym Gene Name

hALP

Product Synonym Names

NAT10; N-acetyltransferase 10; ALP; DKFZp434C116; FLJ10774; FLJ12179; FLJ23850; KIAA1709; hALP; N-acetyltransferase-like; N-acetyltransferase-like protein; NAT10 antibody; N-acetyltransferase 10 antibody; ALP antibody; DKFZp434C116 antibody; FLJ10774 antibody; FLJ12179 antibody; FLJ23850 antibody; KIAA1709 antibody; hALP antibody; N-acetyltransferase-like antibody; N-acetyltransferase-like protein antibody; NAT10 / hALP

Immunogen Sequence

KSDKKRKLEA KQ

Clonality

Polyclonal

Host

Goat

Species Reactivity

Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog

Form/Format

Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Purity/Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

100ug specific antibody in 200ul (lot specific)

Preparation and Storage

Aliquot and store at -20 degree C. Minimize freezing and thawing.

Antibody/Peptide Pairs

NAT10 / hALP peptide (MBS425332) is used for blocking the activity of NAT10/hALP antibody (MBS420570)

Molecular Weight

107,297 Da

OMIM

609221

Immunogen

Peptide with sequence C-KSDKKRKLEAKQ, from the internal region (near the C Terminus) of the protein sequence according to NP_078938.1.

Epitope

Internal region (near the C Terminus)

ISO Certification

Manufactured in an ISO 9001:2008 Certified Laboratory.

Applications Tested/Suitable for anti-NAT10 antibody

Peptide ELISA (EIA), Immunohistochemistry (IHC)

Application Notes for anti-NAT10 antibody

Peptide ELISA: Antibody detection limit dilution 1: 64000.
Immunohistochemistry: In paraffin embedded Human Colon shows nuclear staining in absorptive cells. Recommended concentration, 3-6ug/ml.
Western Blot: Preliminary experiments gave an approx 26kDa band in Human Brain (Cerebellum, Frontal Cortex and Hippocampus) lysates after 0. 01ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 116kDa according to NP_078938.1. The 26kDa band was successfully blocked by incubation with the immunizing peptide.

Other Notes

Small volumes of anti-NAT10 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for NAT10. It may not necessarily be applicable to this product.

NCBI GI #

13399322

NCBI GeneID

55226

NCBI Accession #

NP_078938.1

UniProt Secondary Accession #

Q86WK5; Q9C0F4; Q9HA61; Q9NVF2; B4DFD5; E7ESU4; E9PMN9

UniProt Related Accession #

Q9H0A0

GenBank Nucleotide #

NM_024662.1

NCBI Official Full Name

N-acetyltransferase 10 (GCN5-related)

NCBI Official Synonym Full Names

N-acetyltransferase 10

NCBI Official Symbol

NAT10

NCBI Official Synonym Symbols

ALP; NET43

UniProt Protein Name

RNA cytidine acetyltransferase

UniProt Synonym Protein Names

18S rRNA cytosine acetyltransferase

Protein Family

Putative nucleobase-ascorbate transporter

UniProt Gene Name

NAT10

UniProt Entry Name

NAT10_HUMAN

NCBI Protein Information

RNA cytidine acetyltransferase

UniProt Comments for NAT10

ALP: Has protein acetyltransferase activity in vitro. Can acetylate both histones and microtubules. Histone acetylation may regulate transcription and mitotic chromosome de-condensation. Activates telomerase activity by stimulating the transcription of TERT, and may also regulate telomerase function by affecting the balance of telomerase subunit assembly, disassembly, and localization. Acetylates alpha-tubulin, which may affect microtubule stability and cell division. Belongs to the UPF0202 family.

Protein type: Acetyltransferase; Nucleolus; EC 2.3.1.-

Chromosomal Location of Human Ortholog: 11p13

Cellular Component: membrane; nuclear chromosome, telomeric region; nucleolus; nucleoplasm; nucleus; telomerase holoenzyme complex

Molecular Function: ATP binding; N-acetyltransferase activity; protein binding

Biological Process: negative regulation of telomere maintenance via telomerase; rRNA modification; tRNA processing

Research Articles on NAT10

1. The subcellular redistribution of NAT10 can be induced by decreases in GSK-3b activity. This redistribution increases cancer cell motility, and is, thus, correlated with invasive potential and poorer clinical outcome

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-NAT10 antibody

Products by Pathway	Pathway Diagram
Gene Expression Pathway antibodies	Gene Expression Pathway Diagram
Ribosome Biogenesis In Eukaryotes Pathway antibodies	Ribosome Biogenesis In Eukaryotes Pathway Diagram
Ribosome Biogenesis In Eukaryotes Pathway antibodies	Ribosome Biogenesis In Eukaryotes Pathway Diagram
Metapathway Biotransformation antibodies	Metapathway Biotransformation Diagram
RRNA Modification In The Nucleus Pathway antibodies	RRNA Modification In The Nucleus Pathway Diagram
RRNA Processing Pathway antibodies	RRNA Processing Pathway Diagram

Diseases associated with anti-NAT10 antibody

Disease Name	Pubmed Publications
Ovarian Diseases Antibodies	>1 publications with NAT10 and Ovarian Diseases
Carcinoma, Hepatocellular Antibodies	>1 publications with NAT10 and Carcinoma, Hepatocellular
Neoplasms, Experimental Antibodies	>1 publications with NAT10 and Neoplasms, Experimental
Neoplasm Metastasis Antibodies	>1 publications with NAT10 and Neoplasm Metastasis
Lung Neoplasms Antibodies	>1 publications with NAT10 and Lung Neoplasms
Neoplasm Invasiveness Antibodies	>1 publications with NAT10 and Neoplasm Invasiveness

Organs/Tissues associated with anti-NAT10 antibody

Organ/Tissue Name	Pubmed Publications
Ovary Antibodies	>1 publications with NAT10 and Ovary
Lung Antibodies	>1 publications with NAT10 and Lung