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GRAF/ARHGAP26 (ARHGAP26) Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS420578

MBS420578 is an antibody targeting ARHGAP26. Also known as Goat anti-GRAF/ARHGAP26 Antibody. Reactive in Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow. Host: Goat. More details

$450 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
GRAF/ARHGAP26, Polyclonal Antibody
Full Product Name
Goat anti-GRAF/ARHGAP26 Antibody
Product Gene Name
Product Synonym Gene Name
GRAF
Product Synonym Names
ARHGAP26; GRAF; Rho GTPase activating protein 26; KIAA0621; FLJ42530; OPHN1L; OPHN1L1; GTPase regulator associated with FAK; GTPase regulator associated with focal adhesion kinase pp125(FAK); GTPase regulator associated with the focal adhesion kinase pp1; ARHGAP26 antibody; GRAF antibody; Rho GTPase activating protein 26 antibody; KIAA0621 antibody; FLJ42530 antibody; OPHN1L antibody; OPHN1L1 antibody; GTPase regulator associated with FAK antibody; GTPase regulator associated with focal adhesion kinase pp125(FAK) antibody; GTPase regulator associated with the focal adhesion kinase pp125 antibody; GRAF / ARHGAP26
Immunogen Sequence
KTGLIPENYV EFL
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
100ug specific antibody in 200ul (lot specific)
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Antibody/Peptide Pairs
Molecular Weight
86,165 Da
OMIM
605370
Immunogen
Peptide with sequence C-KTGLIPENYVEFL, from the C Terminus of the protein sequence according to NP_055886.1; NP_001129080.1.
Epitope
C Terminus
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Applications Tested/Suitable for anti-ARHGAP26 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes for anti-ARHGAP26 antibody
Peptide ELISA: Antibody detection limit dilution 1: 64000.
Western Blot: Approx 90kDa band observed in Human Heart lysates (calculated MW of 92.2kDa according to NP_055886.1). Recommended concentration: 0.3-1ug/ml. An additional strong band of unknown identity was also consistently observed at 16kDa. This band was successfully blocked by incubation with the immunising peptide.

Western Blot (WB) of anti-ARHGAP26 antibody

(0.3ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.
Goat Anti-ARHGAP26 Polyclonal Antibody – Cat# MBS420578

Other Notes

Small volumes of anti-ARHGAP26 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for ARHGAP26. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI Related Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
rho GTPase-activating protein 26 isoform a
NCBI Official Synonym Full Names
Rho GTPase activating protein 26
NCBI Official Symbol
ARHGAP26
NCBI Official Synonym Symbols
GRAF; GRAF1; OPHN1L; OPHN1L1
UniProt Protein Name
Rho GTPase-activating protein 26
UniProt Synonym Protein Names
GTPase regulator associated with focal adhesion kinase; Oligophrenin-1-like protein; Rho-type GTPase-activating protein 26
UniProt Gene Name
ARHGAP26
UniProt Synonym Gene Names
GRAF; KIAA0621; OPHN1L
UniProt Entry Name
RHG26_HUMAN
NCBI Protein Information
rho GTPase-activating protein 26

NCBI Summary for ARHGAP26

Interaction of a cell with the extracellular matrix triggers integrin cell surface receptors to begin signaling cascades that regulate the organization of the actin-cytoskeleton. One of the proteins involved in these cascades is focal adhesion kinase. The protein encoded by this gene is a GTPase activating protein that binds to focal adhesion kinase and mediates the activity of the GTP binding proteins RhoA and Cdc42. Defects in this gene are a cause of juvenile myelomonocytic leukemia (JMML). Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2010]
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UniProt Comments for ARHGAP26

ARHGAP26: GTPase-activating protein for RHOA and CDC42. Interacts with NYAP1, NYAP2 and MYO16. Binds to the C-terminus of PTK2/FAK1. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Oncoprotein; GAPs; GAPs, Rac/Rho

Chromosomal Location of Human Ortholog: 5q31

Cellular Component: cytoskeleton; cytosol; focal adhesion

Molecular Function: GTPase activator activity; phospholipid binding; protein binding

Biological Process: actin cytoskeleton organization and biogenesis; nervous system development; positive regulation of GTPase activity; regulation of small GTPase mediated signal transduction; signal transduction

Disease: Juvenile Myelomonocytic Leukemia
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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