Goat Anti-PCK2 Polyclonal Antibody – Cat# MBS420663

(2ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

PCK2 Antibody – Goat Polyclonal

Name: PCK2 Antibody; Polyclonal; Goat; MyBioSource
Brand: MyBioSource
SKU: MBS420663
Price: 450 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS420663

Phosphoenolpyruvate carboxykinase 2 - PK2 or PEPCK - is a ubiquitously expressed enzyme across all life forms. In mammalian species, mouse, human or rabbit, the protein resides in 2 isoforms, cytosolic and mitochondrial, and is a rate limiting enzyme in… More details

$450 / 0.1 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

PCK2/PEPCK-M, Polyclonal Antibody

Full Product Name

Goat anti-PCK2/PEPCK-M Antibody

Product Gene Name

anti-PCK2 antibody

Product Synonym Gene Name

PEPCK-M

Product Synonym Names

PCK2; PEPCK-M; phosphoenolpyruvate carboxykinase 2 (mitochondrial); HGNC: 8725; PEPCK; PEPCK2; PEP carboxykinase; mitochondrial phosphoenolpyruvate carboxykinase 2; phosphoenolpyruvate carboxylase; phosphopyruvate carboxylase; PCK2 antibody; PEPCK-M antibody; phosphoenolpyruvate carboxykinase 2 (mitochondrial) antibody; HGNC: 8725 antibody; PEPCK antibody; PEPCK2 antibody; PEP carboxykinase antibody; mitochondrial phosphoenolpyruvate carboxykinase 2 antibody; phosphoenolpyruvate carboxylase antibody; phosphopyruvate carboxylase antibody; PCK2 / PEPCK-M

Immunogen Sequence

KPWKPGDKEP CAH

Clonality

Polyclonal

Host

Goat

Species Reactivity

Tested: Human, Mouse, Rat, Guinea Pig; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow

Form/Format

Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Purity/Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

100ug specific antibody in 200ul (lot specific)

Preparation and Storage

Aliquot and store at -20 degree C. Minimize freezing and thawing.

Antibody/Peptide Pairs

PCK2 / PEPCK-M peptide (MBS426107) is used for blocking the activity of PCK2/PEPCK-M antibody (MBS420663)

Molecular Weight

47,564 Da

OMIM

261650

Immunogen

Peptide with sequence KPWKPGDKEPCAH, from the internal region of the protein sequence according to NP_004554.2.

Epitope

Internal region

Note

This antibody is expected to recognise isoform 1 (NP_004554) only, no cross-reactivity with PCK1.

ISO Certification

Manufactured in an ISO 9001:2008 Certified Laboratory.

Applications Tested/Suitable for anti-PCK2 antibody

Peptide ELISA (EIA), Western Blot (WB)

Application Notes for anti-PCK2 antibody

Peptide ELISA: Antibody detection limit dilution 1: 4000.
Western Blot: Approx 75kDa band observed in Human Liver lysates (calculated MW of 70.7kDa according to NP_004554.2). Recommended concentration: 2-4ug/ml. Confirmation plus additional tested rodent species was recently puiblished: PMID: 23466304.

Other Notes

Small volumes of anti-PCK2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for PCK2. It may not necessarily be applicable to this product.

NCBI GI #

66346721

NCBI GeneID

5106

NCBI Accession #

NP_004554.2

UniProt Secondary Accession #

O43253; Q86U01; Q9BV62

UniProt Related Accession #

Q16822

GenBank Nucleotide #

NM_004563.2

NCBI Official Full Name

phosphoenolpyruvate carboxykinase

NCBI Official Synonym Full Names

phosphoenolpyruvate carboxykinase 2, mitochondrial

NCBI Official Symbol

PCK2

NCBI Official Synonym Symbols

PEPCK; PEPCK2; PEPCK-M

UniProt Protein Name

Phosphoenolpyruvate carboxykinase [GTP], mitochondrial

Protein Family

Phosphoenolpyruvate carboxykinase

UniProt Gene Name

PCK2

UniProt Synonym Gene Names

PEPCK2; PEPCK-M

UniProt Entry Name

PCKGM_HUMAN

NCBI Protein Information

phosphoenolpyruvate carboxykinase [GTP], mitochondrial

NCBI Summary for PCK2

This gene encodes a mitochondrial enzyme that catalyzes the conversion of oxaloacetate to phosphoenolpyruvate in the presence of guanosine triphosphate (GTP). A cytosolic form of this protein is encoded by a different gene and is the key enzyme of gluconeogenesis in the liver. Alternatively spliced transcript variants have been described. [provided by RefSeq, Apr 2014]

UniProt Comments for PCK2

PCK2: Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle. Monomer. Belongs to the phosphoenolpyruvate carboxykinase [GTP] family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Lyase; Kinase, other; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Carbohydrate Metabolism - pyruvate; Carbohydrate Metabolism - citrate (TCA) cycle; EC 4.1.1.32; Mitochondrial

Chromosomal Location of Human Ortholog: 14q11.2

Cellular Component: mitochondrial matrix; mitochondrion

Molecular Function: GTP binding; metal ion binding; phosphoenolpyruvate carboxykinase (GTP) activity; phosphoenolpyruvate carboxykinase activity; protein binding

Biological Process: gluconeogenesis

Disease: Phosphoenolpyruvate Carboxykinase Deficiency, Mitochondrial

Research Articles on PCK2

1. Results indicate that PEPCK promotes tumor growth by increasing glucose and glutamine metabolism, increases anabolic metabolism and promotes mTORC1 activity.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-PCK2 antibody

Products by Pathway	Pathway Diagram
AMPK Signaling Pathway antibodies	AMPK Signaling Pathway Diagram
AMPK Signaling Pathway antibodies	AMPK Signaling Pathway Diagram
Adipocytokine Signaling Pathway antibodies	Adipocytokine Signaling Pathway Diagram
Adipocytokine Signaling Pathway antibodies	Adipocytokine Signaling Pathway Diagram
Adipogenesis Pathway antibodies	Adipogenesis Pathway Diagram
Citrate Cycle (TCA Cycle) Pathway antibodies	Citrate Cycle (TCA Cycle) Pathway Diagram
Citrate Cycle (TCA Cycle) Pathway antibodies	Citrate Cycle (TCA Cycle) Pathway Diagram
DNA Damage Response (only ATM Dependent) Pathway antibodies	DNA Damage Response (only ATM Dependent) Pathway Diagram
FoxO Signaling Pathway antibodies	FoxO Signaling Pathway Diagram
Glucagon Signaling Pathway antibodies	Glucagon Signaling Pathway Diagram

Diseases associated with anti-PCK2 antibody

Disease Name	Pubmed Publications
Fatty Liver Antibodies	>8 publications with PCK2 and Fatty Liver
Liver Diseases Antibodies	>7 publications with PCK2 and Liver Diseases
Liver Neoplasms Antibodies	>4 publications with PCK2 and Liver Neoplasms
Hepatitis Antibodies	>4 publications with PCK2 and Hepatitis
Carcinoma, Hepatocellular Antibodies	>4 publications with PCK2 and Carcinoma, Hepatocellular
Adenocarcinoma Antibodies	>3 publications with PCK2 and Adenocarcinoma
Lung Neoplasms Antibodies	>3 publications with PCK2 and Lung Neoplasms
Inflammation Antibodies	>2 publications with PCK2 and Inflammation
Necrosis Antibodies	>2 publications with PCK2 and Necrosis
Disease Models, Animal Antibodies	>2 publications with PCK2 and Disease Models, Animal

Organs/Tissues associated with anti-PCK2 antibody

Organ/Tissue Name	Pubmed Publications
Liver Antibodies	>28 publications with PCK2 and Liver
Blood Antibodies	>18 publications with PCK2 and Blood
Adipose Tissue Antibodies	>9 publications with PCK2 and Adipose Tissue
Muscle Antibodies	>6 publications with PCK2 and Muscle
Connective Tissue Antibodies	>5 publications with PCK2 and Connective Tissue
Kidney Antibodies	>5 publications with PCK2 and Kidney
Embryonic Tissue Antibodies	>4 publications with PCK2 and Embryonic Tissue
Lung Antibodies	>3 publications with PCK2 and Lung
Pancreas Antibodies	>3 publications with PCK2 and Pancreas
Vascular Antibodies	>2 publications with PCK2 and Vascular