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ARP1 Homolog B (ACTR1B) Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS420714

MBS420714 is an antibody targeting ACTR1B. Also known as Goat anti-ARP1 homolog B Antibody. Reactive in Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow. Host: Goat. More details

$450 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
ARP1 homolog B (ACTR1B), Polyclonal Antibody
Full Product Name
Goat anti-ARP1 homolog B Antibody
Product Gene Name
Product Synonym Names
ACTR1B; ARP1B; CTRN2; ARP1 actin-related protein 1 homolog B, centractin beta (yeast); PC3; centractin beta; ARP1, yeast homolog B; ARP1 (actin-related protein 1, yeast) homolog B (centractin beta); ARP1 actin-related protein 1 homolog B, centractin beta; ACTR1B antibody; ARP1B antibody; CTRN2 antibody; ARP1 actin-related protein 1 homolog B; centractin beta (yeast) antibody; PC3 antibody; centractin beta antibody; ARP1; yeast homolog B antibody; ARP1 (actin-related protein 1; yeast) homolog B (centractin beta) antibody; ARP1 actin-related protein 1 homolog B; centractin beta antibody; ARP1 homolog B; ARP1 homolog B
Immunogen Sequence
YEEDGSRAIH RKT
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
100ug specific antibody in 200ul (lot specific)
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Antibody/Peptide Pairs
Molecular Weight
42,293 Da
OMIM
605144
Immunogen
Peptide with sequence C-YEEDGSRAIHRKT, from the C Terminus of the protein sequence according to NP_005726.1.
Epitope
C Terminus
Note
This epitope was selected to minimise the chance of cross-reactivity with ACTR1A (YEEDGARSIHRKT).
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Applications Tested/Suitable for anti-ACTR1B antibody
Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-ACTR1B antibody
Peptide ELISA: Antibody detection limit dilution 1: 128000.
Immunohistochemistry: In paraffin embedded Human Uterus shows fibrillar staining of cytoplasm in endometrial cells. Recommended concentration, 3-5ug/ml.
Western Blot: Approx 45kDa band observed in human heart and brain lysates (predicted MW of 43kDa according to NP_005726). Recommended for use at 0.5-2ug/ml.

Western Blot (WB) of anti-ACTR1B antibody

Staining (1ug/ml) of human heart lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Goat Anti-ACTR1B Polyclonal Antibody – Cat# MBS420714

Immunohistochemistry (IHC) of anti-ACTR1B antibody

(3.8ug/ml) staining of paraffin embedded Human Uterus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Goat Anti-ACTR1B Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS420714

Other Notes

Small volumes of anti-ACTR1B antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for ACTR1B. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
beta-centractin
NCBI Official Synonym Full Names
ARP1 actin-related protein 1 homolog B, centractin beta
NCBI Official Symbol
ACTR1B
NCBI Official Synonym Symbols
PC3; ARP1B; CTRN2
UniProt Protein Name
Beta-centractin
UniProt Synonym Protein Names
Actin-related protein 1B; ARP1B
Protein Family
UniProt Gene Name
ACTR1B
UniProt Synonym Gene Names
CTRN2; ARP1B
UniProt Entry Name
ACTY_HUMAN
NCBI Protein Information
beta-centractin

NCBI Summary for ACTR1B

This gene encodes a 42.3 kD subunit of dynactin, a macromolecular complex consisting of 10 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein and is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. This subunit, like ACTR1A, is an actin-related protein. These two proteins, which are of equal length and share 90% amino acid identity, are present in a constant ratio of approximately 1:15 in the dynactin complex. [provided by RefSeq, Aug 2008]
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UniProt Comments for ACTR1B

ACTR1B: Component of a multi-subunit complex involved in microtubule based vesicle motility. It is associated with the centrosome. Belongs to the actin family. ARP1 subfamily.

Protein type: Cytoskeletal; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 2q11.2

Cellular Component: centrosome; cytoplasm; cytosol; dynactin complex; membrane; microtubule cytoskeleton

Molecular Function: ATP binding; protein binding

Biological Process: antigen processing and presentation of exogenous peptide antigen via MHC class II
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Diseases associated with anti-ACTR1B antibody

Organs/Tissues associated with anti-ACTR1B antibody

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