Goat Anti-TIMM50 Polyclonal Antibody – Cat# MBS420718

Staining (0.5ug/ml) of Human Heart lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

TIMM50 Antibody – Goat Polyclonal

Name: TIMM50 Antibody; Polyclonal; Goat; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS420718
Price: 450 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS420718

MBS420718 is an antibody targeting TIMM50. Also known as Goat anti-TIM50 Antibody. Reactive in Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow. Host: Goat. More details

$450 / 0.1 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

TIM50 (TIMM50), Polyclonal Antibody

Full Product Name

Goat anti-TIM50 Antibody

Product Gene Name

anti-TIMM50 antibody

Product Synonym Gene Name

TIM50

Product Synonym Names

TIMM50; TIM50L; translocase of inner mitochondrial membrane 50 homolog (yeast); Tim50-like protein; homolog of yeast Tim50; translocase of inner mitochondrial membrane 50 homolog (S. cerevisiae); MGC102733; TIM50; TIMM50 antibody; TIM50L antibody; translocase of inner mitochondrial membrane 50 homolog (yeast) antibody; Tim50-like protein antibody; homolog of yeast Tim50 antibody; translocase of inner mitochondrial membrane 50 homolog (S. cerevisiae) antibody; MGC102733 antibody; TIM50 antibody

Immunogen Sequence

CDVRTVLEHY ALEDD

Clonality

Polyclonal

Host

Goat

Species Reactivity

Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow

Form/Format

Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Purity/Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

100ug specific antibody in 200ul (lot specific)

Preparation and Storage

Aliquot and store at -20 degree C. Minimize freezing and thawing.

Antibody/Peptide Pairs

TIM50 peptide (MBS425520) is used for blocking the activity of TIM50 antibody (MBS420718)

Molecular Weight

27,579 Da

OMIM

607381

Immunogen

Peptide with sequence CDVRTVLEHYALEDD, from the internal region of the protein sequence according to NP_001001563.1.

Epitope

Internal region

Note

This antibody is expected to recognise an epitope corresponding to aa 396-409 of human TIM50.

ISO Certification

Manufactured in an ISO 9001:2008 Certified Laboratory.

Applications Tested/Suitable for anti-TIMM50 antibody

Peptide ELISA (EIA), Western Blot (WB)

Application Notes for anti-TIMM50 antibody

Peptide ELISA: Antibody detection limit dilution 1: 16000.
Western Blot: Experiments gave bands at approx 40kDa in Human Heart lysate after 0.5ug/ml antibody staining. This band corresponds to earlier findings in literature with different antibodies (PMID: 15044455). This protein has a calculated MW of 50.5kDa according to NP_001001563. Recommended concentration: 0.5-3ug/ml.

Other Notes

Small volumes of anti-TIMM50 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for TIMM50. It may not necessarily be applicable to this product.

NCBI GI #

48526509

NCBI GeneID

92609

NCBI Accession #

NP_001001563.1

UniProt Secondary Accession #

Q330K1; Q6QA00; Q96FJ5; Q96GY2; Q9H370

UniProt Related Accession #

Q3ZCQ8

GenBank Nucleotide #

NM_001001563.2

NCBI Official Full Name

mitochondrial import inner membrane translocase subunit TIM50

NCBI Official Synonym Full Names

translocase of inner mitochondrial membrane 50

NCBI Official Symbol

TIMM50

NCBI Official Synonym Symbols

TIM50; TIM50L

UniProt Protein Name

Mitochondrial import inner membrane translocase subunit TIM50

Protein Family

Mitochondrial import inner membrane translocase

UniProt Gene Name

TIMM50

UniProt Synonym Gene Names

TIM50

UniProt Entry Name

TIM50_HUMAN

NCBI Protein Information

mitochondrial import inner membrane translocase subunit TIM50

UniProt Comments for TIMM50

TIMM50: Essential component of the TIM23 complex, a complex that mediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane. Has some phosphatase activity in vitro; however such activity may not be relevant in vivo. Isoform 2 may participate in the release of snRNPs and SMN from the Cajal body. Belongs to the TIM50 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Mitochondrial; Membrane protein, integral; Protein phosphatase, dual-specificity

Chromosomal Location of Human Ortholog: 19q13.2

Cellular Component: integral to membrane; mitochondrial inner membrane; mitochondrial inner membrane presequence translocase complex; mitochondrion; nuclear speck; nucleoplasm

Molecular Function: interleukin-2 receptor binding; phosphoprotein phosphatase activity; protein binding; protein serine/threonine phosphatase activity; protein tyrosine phosphatase activity; ribonucleoprotein binding; RNA binding

Biological Process: mitochondrial membrane organization and biogenesis; protein amino acid dephosphorylation; protein import into mitochondrial matrix; release of cytochrome c from mitochondria

Research Articles on TIMM50

1. the interaction of divalent metal ions with the intermembrane space domain of Tim50 (Tim50(IMS)) and the interaction of presequence peptides with Tim50(IMS) in presence of Ca(2+) ion was investigated.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-TIMM50 antibody

Products by Pathway	Pathway Diagram
Metabolism Of Proteins Pathway antibodies	Metabolism Of Proteins Pathway Diagram
Mitochondrial Protein Import Pathway antibodies	Mitochondrial Protein Import Pathway Diagram

Organs/Tissues associated with anti-TIMM50 antibody

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>2 publications with TIMM50 and Brain
Thyroid Antibodies	>1 publications with TIMM50 and Thyroid
Intestine Antibodies	>1 publications with TIMM50 and Intestine
Adrenal Gland Antibodies	>1 publications with TIMM50 and Adrenal Gland
Liver Antibodies	>1 publications with TIMM50 and Liver
Lung Antibodies	>1 publications with TIMM50 and Lung