Goat Anti-MCL1 Polyclonal Antibody – Cat# MBS420762

Staining (1ug/ml) of Human Lung lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

MCL1 Antibody – Goat Polyclonal

Name: MCL1 Antibody; Polyclonal; Goat; Human/Mouse/Rat-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS420762
Price: 450 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS420762

MBS420762 is an antibody targeting MCL1. Also known as Goat anti-MCL1 Antibody. Reactive in Tested: Human, Mouse, Rat; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow. Host: Goat. More details

$450 / 0.1 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

MCL1, Polyclonal Antibody

Full Product Name

Goat anti-MCL1 Antibody

Product Gene Name

anti-MCL1 antibody

Product Synonym Names

MCL1; TM; EAT; MCL1L; MCL1S; MGC1839; MGC104264; myeloid cell leukemia sequence 1 (BCL2-related); induced myeloid leukemia cell differentiation protein Mcl-1; myeloid cell leukemia sequence 1; myeloid cell leukemia sequence 1, isoform 1; BCL2L3; Mcl-1; MCL1 antibody; TM antibody; EAT antibody; MCL1L antibody; MCL1S antibody; MGC1839 antibody; MGC104264 antibody; myeloid cell leukemia sequence 1 (BCL2-related) antibody; induced myeloid leukemia cell differentiation protein Mcl-1 antibody; myeloid cell leukemia sequence 1 antibody; myeloid cell leukemia sequence 1; isoform 1 antibody; BCL2L3 antibody; Mcl-1 antibody

Immunogen Sequence

AGVGAGLAYL IR

Clonality

Polyclonal

Host

Goat

Species Reactivity

Tested: Human, Mouse, Rat; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow

Form/Format

Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Purity/Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

100ug specific antibody in 200ul (lot specific)

Preparation and Storage

Aliquot and store at -20 degree C. Minimize freezing and thawing.

Antibody/Peptide Pairs

MCL1 peptide (MBS425071) is used for blocking the activity of MCL1 antibody (MBS420762)

Molecular Weight

28,662 Da

OMIM

159552

Immunogen

Peptide with sequence C-AGVGAGLAYLIR, from the C Terminus of the protein sequence according to NP_068779.1; NP_001184249.1.

Epitope

C Terminus

Note

This antibody is expected to recognise reported isoform 1 (NP_068779.1) and isoform 3 (NP_001184249.1).

ISO Certification

Manufactured in an ISO 9001:2008 Certified Laboratory.

Applications Tested/Suitable for anti-MCL1 antibody

Peptide ELISA (EIA), Western Blot (WB)

Application Notes for anti-MCL1 antibody

Peptide ELISA: Antibody detection limit dilution 1: 2000.
Western Blot: Approx 35kDa band observed in lysates of Human Lung, in Mouse and Rat Thymus, and in cell line K562 (calculated MW of 37.3kDa according to NP_068779.1). Recommended concentration: 1-3ug/ml.

Other Notes

Small volumes of anti-MCL1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for MCL1. It may not necessarily be applicable to this product.

NCBI GI #

11386165

NCBI GeneID

4170

NCBI Accession #

NP_068779.1

NCBI Related Accession #

NP_001184249.1

UniProt Secondary Accession #

Q9HD91; Q9NRQ3; Q9NRQ4; Q9UHR7; Q9UHR8; Q9UHR9; Q9UNJ1; B2R6B2; D3DV03; D3DV04

UniProt Related Accession #

Q07820

GenBank Nucleotide #

NM_021960.4

NCBI Official Full Name

induced myeloid leukemia cell differentiation protein Mcl-1 isoform 1

NCBI Official Synonym Full Names

myeloid cell leukemia 1

NCBI Official Symbol

MCL1

NCBI Official Synonym Symbols

TM; EAT; MCL1L; MCL1S; Mcl-1; BCL2L3; MCL1-ES; bcl2-L-3; mcl1/EAT

UniProt Protein Name

Induced myeloid leukemia cell differentiation protein Mcl-1

UniProt Synonym Protein Names

Bcl-2-like protein 3; Bcl2-L-3; Bcl-2-related protein EAT/mcl1; mcl1/EAT

Protein Family

Induced myeloid leukemia cell differentiation protein

UniProt Gene Name

MCL1

UniProt Synonym Gene Names

BCL2L3; Bcl2-L-3

UniProt Entry Name

MCL1_HUMAN

NCBI Protein Information

induced myeloid leukemia cell differentiation protein Mcl-1

NCBI Summary for MCL1

This gene encodes an anti-apoptotic protein, which is a member of the Bcl-2 family. Alternative splicing results in multiple transcript variants. The longest gene product (isoform 1) enhances cell survival by inhibiting apoptosis while the alternatively spliced shorter gene products (isoform 2 and isoform 3) promote apoptosis and are death-inducing. [provided by RefSeq, Oct 2010]

UniProt Comments for MCL1

MCL1: a myeloid cell leukemia protein of the Bcl-2 family of proteins. Two alternatively spliced transcripts encoding distinct isoforms have been identified. The longer gene product (isoform 1) enhances cell survival by inhibiting apoptosis while the alternatively spliced shorter gene product (isoform 2) promotes apoptosis and is death-inducing.

Protein type: Inhibitor; Apoptosis; Membrane protein, integral; Channel, misc.; Mitochondrial

Chromosomal Location of Human Ortholog: 1q21

Cellular Component: cytoplasm; cytosol; integral to membrane; membrane; mitochondrial matrix; mitochondrial outer membrane; mitochondrion; nucleoplasm; nucleus

Molecular Function: BH3 domain binding; protein binding; protein channel activity; protein heterodimerization activity; protein homodimerization activity

Biological Process: apoptotic mitochondrial changes; cell fate determination; cellular homeostasis; DNA damage response, signal transduction resulting in induction of apoptosis; multicellular organismal development; response to cytokine stimulus

Research Articles on MCL1

1. Results indicate that Mcl-1 expression induces epithelial-mesenchymal transition via beta-catenin, MEK1/2 and MAPK signaling pathways, which subsequently stimulates the invasive and migratory capacity of human gastric cancer cells.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-MCL1 antibody

Reference Product	PubMed Publications
BAX antibody	>233 publications with MCL1 and BAX
BCL2L11 antibody	>93 publications with MCL1 and BCL2L11
PMAIP1 antibody	>60 publications with MCL1 and PMAIP1
BAK1 antibody	>49 publications with MCL1 and BAK1
BID antibody	>42 publications with MCL1 and BID
TP53 antibody	>29 publications with MCL1 and TP53
BBC3 antibody	>25 publications with MCL1 and BBC3
USP9X antibody	>14 publications with MCL1 and USP9X
HUWE1 antibody	>6 publications with MCL1 and HUWE1
UBC antibody	>1 publications with MCL1 and UBC

Pathways associated with anti-MCL1 antibody

Products by Pathway	Pathway Diagram
Apoptosis Pathway antibodies	Apoptosis Pathway Diagram
Apoptosis Pathway antibodies	Apoptosis Pathway Diagram
Apoptosis Pathway antibodies	Apoptosis Pathway Diagram
Apoptosis Modulation And Signaling Pathway antibodies	Apoptosis Modulation And Signaling Pathway Diagram
Direct P53 Effectors Pathway antibodies	Direct P53 Effectors Pathway Diagram
E2F Transcription Factor Network Pathway antibodies	E2F Transcription Factor Network Pathway Diagram
HIF-1-alpha Transcription Factor Network Pathway antibodies	HIF-1-alpha Transcription Factor Network Pathway Diagram
IL-7 Signaling Pathway antibodies	IL-7 Signaling Pathway Diagram
IL6-mediated Signaling Events Pathway antibodies	IL6-mediated Signaling Events Pathway Diagram
Jak-STAT Signaling Pathway antibodies	Jak-STAT Signaling Pathway Diagram

Diseases associated with anti-MCL1 antibody

Disease Name	Pubmed Publications
Lung Neoplasms Antibodies	>72 publications with MCL1 and Lung Neoplasms
Lung Diseases Antibodies	>71 publications with MCL1 and Lung Diseases
Necrosis Antibodies	>56 publications with MCL1 and Necrosis
Breast Neoplasms Antibodies	>55 publications with MCL1 and Breast Neoplasms
Liver Diseases Antibodies	>50 publications with MCL1 and Liver Diseases
Multiple Myeloma Antibodies	>43 publications with MCL1 and Multiple Myeloma
Liver Neoplasms Antibodies	>40 publications with MCL1 and Liver Neoplasms
Carcinoma, Hepatocellular Antibodies	>38 publications with MCL1 and Carcinoma, Hepatocellular
Neoplasms, Experimental Antibodies	>37 publications with MCL1 and Neoplasms, Experimental
Inflammation Antibodies	>32 publications with MCL1 and Inflammation

Organs/Tissues associated with anti-MCL1 antibody

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>184 publications with MCL1 and Blood
Lung Antibodies	>99 publications with MCL1 and Lung
Liver Antibodies	>84 publications with MCL1 and Liver
Bone Antibodies	>73 publications with MCL1 and Bone
Bone Marrow Antibodies	>57 publications with MCL1 and Bone Marrow
Brain Antibodies	>49 publications with MCL1 and Brain
Prostate Antibodies	>41 publications with MCL1 and Prostate
Skin Antibodies	>29 publications with MCL1 and Skin
Vascular Antibodies	>25 publications with MCL1 and Vascular
Lymph Node Antibodies	>19 publications with MCL1 and Lymph Node