Goat Anti-BRUNOL5 Polyclonal Antibody – Cat# MBS420771

(1ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Bruno-Like 5 (BRUNOL5) Antibody – Goat Polyclonal

Name: BRUNOL5 Antibody; Polyclonal; Goat; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS420771
Price: 450 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS420771

MBS420771 is an antibody targeting BRUNOL5. Also known as Goat anti-Bruno-like 5 Antibody. Reactive in Tested: Human; Expected from sequence similarity: Human. Host: Goat. More details

$450 / 0.1 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Bruno-like 5 (BRUNOL5), Polyclonal Antibody

Full Product Name

Goat anti-Bruno-like 5 Antibody

Product Gene Name

anti-BRUNOL5 antibody

Product Synonym Names

BRUNOL5; bruno-like 5, RNA binding protein (Drosophila); BRUNOL-5; CELF5; Bruno (Drosophila) -like 5, RNA binding protein; CUG-BP and ETR-3 like factor 5; RNA-binding protein BRUNOL-5; bruno-like 5, RNA binding protein; BRUNOL5 antibody; bruno-like 5; RNA binding protein (Drosophila) antibody; BRUNOL-5 antibody; CELF5 antibody; Bruno (Drosophila) -like 5; RNA binding protein antibody; CUG-BP and ETR-3 like factor 5 antibody; RNA-binding protein BRUNOL-5 antibody; bruno-like 5; RNA binding protein antibody; Bruno-like 5; Bruno-like 5

Immunogen Sequence

EPPGGQPDGM KD

Clonality

Polyclonal

Host

Goat

Species Reactivity

Tested: Human; Expected from sequence similarity: Human

Form/Format

Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Purity/Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

100ug specific antibody in 200ul (lot specific)

Preparation and Storage

Aliquot and store at -20 degree C. Minimize freezing and thawing.

Antibody/Peptide Pairs

Bruno-like 5 peptide (MBS426083) is used for blocking the activity of Bruno-like 5 antibody (MBS420771)

Molecular Weight

44,479 Da

OMIM

612680

Immunogen

Peptide with sequence C-EPPGGQPDGMKD, from the internal region (near the N Terminus) of the protein sequence according to NP_068757.2.

Epitope

Internal region (near the N Terminus)

ISO Certification

Manufactured in an ISO 9001:2008 Certified Laboratory.

Applications Tested/Suitable for anti-BRUNOL5 antibody

Peptide ELISA (EIA), Western Blot (WB)

Application Notes for anti-BRUNOL5 antibody

Peptide ELISA: Antibody detection limit dilution 1: 1000.
Western Blot: Approx 55kDa band observed in Human Heart lysates (calculated MW of 52.4kDa according to NP_068757.2). Recommended concentration: 1-3ug/ml.

Other Notes

Small volumes of anti-BRUNOL5 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for BRUNOL5. It may not necessarily be applicable to this product.

NCBI GI #

24762232

NCBI GeneID

60680

NCBI Accession #

NP_068757.2

UniProt Secondary Accession #

O75253; Q59GP2; Q86VW6; Q9BZC0; Q9NR86; D6W614

UniProt Related Accession #

Q8N6W0

GenBank Nucleotide #

NM_021938.3

NCBI Official Full Name

CUGBP Elav-like family member 5 isoform 1

NCBI Official Synonym Full Names

CUGBP, Elav-like family member 5

NCBI Official Symbol

CELF5

NCBI Official Synonym Symbols

CELF-5; BRUNOL5; BRUNOL-5

UniProt Protein Name

CUGBP Elav-like family member 5

UniProt Synonym Protein Names

Bruno-like protein 5; CUG-BP- and ETR-3-like factor 5; RNA-binding protein BRUNOL-5

UniProt Gene Name

CELF5

UniProt Synonym Gene Names

BRUNOL5; CELF-5

UniProt Entry Name

CELF5_HUMAN

NCBI Protein Information

CUGBP Elav-like family member 5

NCBI Summary for BRUNOL5

This gene encodes a member of the the CELF/BRUNOL protein family, which contain two N-terminal RNA recognition motif (RRM) domains, one C-terminal RRM domain, and a divergent segment of 160-230 aa between the second and third RRM domains. Members of this protein family regulate pre-mRNA alternative splicing and may also be involved in mRNA editing and translation. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jan 2012]

UniProt Comments for BRUNOL5

CELF5: RNA-binding protein implicated in the regulation of pre- mRNA alternative splicing. Mediates exon inclusion and/or exclusion in pre-mRNA that are subject to tissue-specific and developmentally regulated alternative splicing. Specifically activates exon 5 inclusion of cardiac isoforms of TNNT2 during heart remodeling at the juvenile to adult transition. Binds to muscle-specific splicing enhancer (MSE) intronic sites flanking the alternative exon 5 of TNNT2 pre-mRNA. Belongs to the CELF/BRUNOL family. 2 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 19p13

Cellular Component: cytoplasm; nucleus

Molecular Function: nucleotide binding; RNA binding

Biological Process: mRNA processing

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Organs/Tissues associated with anti-BRUNOL5 antibody

Organ/Tissue Name	Pubmed Publications
Embryonic Tissue Antibodies	>1 publications with BRUNOL5 and Embryonic Tissue
Brain Antibodies	>1 publications with BRUNOL5 and Brain