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Clik1/STK35 (STK35) Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS420787

MBS420787 is an antibody targeting STK35. Also known as Goat anti-Clik1/STK35 Antibody. Reactive in Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow. Host: Goat. More details

$450 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Clik1/STK35, Polyclonal Antibody
Full Product Name
Goat anti-Clik1/STK35 Antibody
Product Gene Name
Product Synonym Gene Name
Clik1
Product Synonym Names
STK35; Clik1; bA550O8.2; bA550O8.2 CLIK1; serine/threonine kinase 35; CLP-36 interacting kinase; CLIK1; STK35L1; OTTHUMP00000030006; serine threonine kinase 35 long form; STK35 antibody; Clik1 antibody; bA550O8.2 antibody; bA550O8.2 CLIK1 antibody; serine/threonine kinase 35 antibody; CLP-36 interacting kinase antibody; CLIK1 antibody; STK35L1 antibody; OTTHUMP00000030006 antibody; serine threonine kinase 35 long form antibody; Clik1 / STK35
Immunogen Sequence
ELETRMDQVT CAA
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
100ug specific antibody in 200ul (lot specific)
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Antibody/Peptide Pairs
Molecular Weight
58,051 Da
OMIM
609370
Immunogen
Peptide with sequence ELETRMDQVTCAA, from the C Terminus of the protein sequence according to NP_543026.2.
Epitope
C Terminus
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Applications Tested/Suitable for anti-STK35 antibody
Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-STK35 antibody
Peptide ELISA: Antibody detection limit dilution 1: 16000.
Immunohistochemistry: In paraffin embedded human testis shows granular cytoplasmic staining of Leydig cells. Recommended concentration, 10ug/ml.
Western Blot: Approx 45kDa band observed in human testis lysates (calculated MW of 44.6kDa according to NP_543026. Recommended concentration: 1-3ug/ml.

Western Blot (WB) of anti-STK35 antibody

(1ug/ml) staining of human testis lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Goat Anti-STK35 Polyclonal Antibody – Cat# MBS420787

Immunohistochemistry (IHC) of anti-STK35 antibody

(10ug/ml) staining of paraffin embedded Human Testis. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.
Goat Anti-STK35 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS420787

Other Notes

Small volumes of anti-STK35 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for STK35. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
serine/threonine-protein kinase 35
NCBI Official Synonym Full Names
serine/threonine kinase 35
NCBI Official Symbol
STK35
NCBI Official Synonym Symbols
CLIK1; STK35L1
UniProt Protein Name
Serine/threonine-protein kinase 35
UniProt Synonym Protein Names
CLP-36-interacting kinase 1; CLIK-1; PDLIM1-interacting kinase 1; Serine/threonine-protein kinase 35 L1
UniProt Gene Name
STK35
UniProt Synonym Gene Names
CLIK1; PDIK1; STK35L1; CLIK-1
UniProt Entry Name
STK35_HUMAN
NCBI Protein Information
serine/threonine-protein kinase 35

NCBI Summary for STK35

The protein encoded by this gene is a kinase that is predominantly found in the nucleus. However, it can interact with PDLIM1/CLP-36 in the cytoplasm and localize to actin stress fibers. The encoded protein may be a regulator of actin stress fibers in nonmuscle cells. [provided by RefSeq, Jul 2008]

UniProt Comments for STK35

CLIK1: is a kinase that is predominantly found in the nucleus. However, it can interact with PDLIM1/CLP-36 in the cytoplasm and localize to actin stress fibers. The encoded protein may be a regulator of actin stress fibers in nonmuscle cells. [provided by RefSeq, Jul 2008]

Protein type: Nucleolus; EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); Protein kinase, Other; Kinase, protein; Other group; NKF4 family

Chromosomal Location of Human Ortholog: 20p13

Cellular Component: cytoplasm; nucleolus

Molecular Function: ATP binding; protein serine/threonine kinase activity

Biological Process: protein amino acid phosphorylation
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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