• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

VPS26A Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS420801

MBS420801 is an antibody targeting VPS26A. Also known as Goat anti-VPS26A Antibody. Reactive in Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Pig, Cow. Host: Goat. More details

$450 / 0.1 mg +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
VPS26A, Polyclonal Antibody
Full Product Name
Goat anti-VPS26A Antibody
Product Gene Name
Product Synonym Names
VPS26A; vacuolar protein sorting 26 homologue A (yeast); FLJ12930; HB58; Hbeta58; VPS26; Hbeta58 homolog; vacuolar protein sorting 26 (yeast homolog); vacuolar protein sorting 26 (yeast); vacuolar protein sorting 26 homolog A; vacuolar protein sorting 26; VPS26A antibody; vacuolar protein sorting 26 homologue A (yeast) antibody; FLJ12930 antibody; HB58 antibody; Hbeta58 antibody; VPS26 antibody; Hbeta58 homolog antibody; vacuolar protein sorting 26 (yeast homolog) antibody; vacuolar protein sorting 26 (yeast) antibody; vacuolar protein sorting 26 homolog A antibody; vacuolar protein sorting 26 homolog A (S. pombe) antibody; PEP8A antibody; OTTHUMP00000019721 antibody; vacuolar protein sorting 26 A antibody
Immunogen Sequence
SPESQASAEQ PEM
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Pig, Cow
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
100ug specific antibody in 200ul (lot specific)
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Antibody/Peptide Pairs
VPS26A peptide (MBS426640) is used for blocking the activity of VPS26A antibody (MBS420801)
Molecular Weight
28,938 Da
OMIM
605506
Immunogen
Peptide with sequence C-SPESQASAEQPEM, from the C Terminus of the protein sequence according to NP_004887.2.
Epitope
C Terminus
Note
This antibody is expected to recognize isoform 1 (NP_004887.2) only.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Applications Tested/Suitable for anti-VPS26A antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes for anti-VPS26A antibody
Peptide ELISA: Antibody detection limit dilution 1: 4000.
Western Blot: Approx 40kDa band observed in Human Kidney lysates (calculated MW of 38.2 kDa according to NP_004887.2). In transfected HEK293 transiently expressing VPS26A a band of approx. 40kDa is observed. This band is not observed in the non-transfected HEK293. Recommended for use at 1-3ug/ml.

Western Blot (WB) of anti-VPS26A antibody

Staining (1ug/ml) of Human Kidney lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Goat Anti-VPS26A Polyclonal Antibody – Cat# MBS420801

Western Blot (WB) of anti-VPS26A antibody

HEK293 overexpressing VPS26A (RC205353) and probed (mock transfection in first lane), tested by Origene.
Goat Anti-VPS26A Polyclonal Antibody, Western Blot (WB) – Cat# MBS420801

Other Notes

Small volumes of anti-VPS26A antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for VPS26A. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
vacuolar protein sorting-associated protein 26A isoform 1
NCBI Official Synonym Full Names
VPS26 retromer complex component A
NCBI Official Symbol
VPS26A
NCBI Official Synonym Symbols
HB58; PEP8A; VPS26; Hbeta58
UniProt Protein Name
Vacuolar protein sorting-associated protein 26A
UniProt Synonym Protein Names
Vesicle protein sorting 26A; hVPS26
UniProt Gene Name
VPS26A
UniProt Synonym Gene Names
VPS26; hVPS26
UniProt Entry Name
VP26A_HUMAN
NCBI Protein Information
vacuolar protein sorting-associated protein 26A

NCBI Summary for VPS26A

This gene belongs to a group of vacuolar protein sorting (VPS) genes. The encoded protein is a component of a large multimeric complex, termed the retromer complex, involved in retrograde transport of proteins from endosomes to the trans-Golgi network. The close structural similarity between the yeast and human proteins that make up this complex suggests a similarity in function. Expression studies in yeast and mammalian cells indicate that this protein interacts directly with VPS35, which serves as the core of the retromer complex. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
Show more

UniProt Comments for VPS26A

VPS26A: Essential component of the retromer complex, a complex required to retrieve lysosomal enzyme receptors (IGF2R and M6PR) from endosomes to the trans-Golgi network. Also required to regulate transcytosis of the polymeric immunoglobulin receptor (pIgR-pIgA). Belongs to the VPS26 family.

Protein type: Vesicle

Chromosomal Location of Human Ortholog: 10q21.1

Cellular Component: cytosol; early endosome; endosome; endosome membrane; intracellular membrane-bound organelle; retromer complex; vesicle

Molecular Function: protein binding; protein transporter activity

Biological Process: intracellular protein transport; regulation of macroautophagy; retrograde transport, endosome to Golgi; Wnt receptor signaling pathway
Show more

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000