Goat Anti-PPP2R5E Polyclonal Antibody – Cat# MBS420815

(0.1ug/ml) staining of Human Cerebellum (A), Mouse (B) and Rat (C) Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

PPP2R5E Antibody – Goat Polyclonal

Name: PPP2R5E Antibody; Polyclonal; Goat; Human/Mouse/Rat-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS420815
Price: 450 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Popular Catalog #: MBS420815

MBS420815 is an antibody targeting PPP2R5E. Also known as Goat anti-PPP2R5E Antibody. Reactive in Tested: Human, Mouse, Rat; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow. Host: Goat. More details

$450 / 0.1 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

PPP2R5E, Polyclonal Antibody

Full Product Name

Goat anti-PPP2R5E Antibody

Product Gene Name

anti-PPP2R5E antibody

Product Synonym Names

PPP2R5E; protein phosphatase 2, regulatory subunit B (B56), epsilon isoform; PP2A, B subunit, B' epsilon isoform; PP2A, B subunit, R5 epsilon isoform; PP2A, B subunit, B56 epsilon isoform; PP2A, B subunit, PR61 epsilon isoform; Serine/threonine protein ph; PPP2R5E antibody; protein phosphatase 2; regulatory subunit B (B56); epsilon isoform antibody; PP2A; B subunit; B\1 epsilon isoform antibody; PP2A; B subunit; R5 epsilon isoform antibody; PP2A; B subunit; B56 epsilon isoform antibody; PP2A; B subunit; PR61 epsilon isoform antibody; Serine/threonine protein phosphatase 2A; 56 kDa regulatory subunit; epsilon isoform antibody; protein phosphatase 2; regulatory subunit B\1; epsilon isoform antibody; PP2A; B subunit; R5 epsilon antibody; epsilon isoform of regulatory subunit B56; protein phosphatase 2A antibody; serine/threonine protein phosphatase 2A; 56 kDa regulatory subunit; epsilon antibody

Immunogen Sequence

LKRGLRRDGI IPT

Clonality

Polyclonal

Host

Goat

Species Reactivity

Tested: Human, Mouse, Rat; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow

Form/Format

Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Purity/Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

100ug specific antibody in 200ul (lot specific)

Preparation and Storage

Aliquot and store at -20 degree C. Minimize freezing and thawing.

Antibody/Peptide Pairs

PPP2R5E peptide (MBS426047) is used for blocking the activity of PPP2R5E antibody (MBS420815)

Molecular Weight

46,180 Da

OMIM

601647

Immunogen

Peptide with sequence C-LKRGLRRDGIIPT, from the C Terminus of the protein sequence according to NP_006237.1.

Epitope

C Terminus

ISO Certification

Manufactured in an ISO 9001:2008 Certified Laboratory.

Applications Tested/Suitable for anti-PPP2R5E antibody

Peptide ELISA (EIA), Western Blot (WB)

Application Notes for anti-PPP2R5E antibody

Peptide ELISA: Antibody detection limit dilution 1: 16000.
Western Blot: Approx 50kDa band observed in Human (Cerebellum), Mouse and Rat Brain lysates (calculated MW of 54.7kDa according to Human NP_001269108.1 and Mouse NP_036154.1 and 50.4kDa according to Rat NP_001100210.1). Recommended concentration: 0.1-0.3ug/ml.

Other Notes

Small volumes of anti-PPP2R5E antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for PPP2R5E. It may not necessarily be applicable to this product.

NCBI GI #

5453956

NCBI GeneID

5529

NCBI Accession #

NP_006237.1

UniProt Secondary Accession #

Q52LW4; A4FU37; B7ZAW5; B7ZKK8; B7ZKK9; J3KQN6

UniProt Related Accession #

Q16537

GenBank Nucleotide #

NM_006246.3

NCBI Official Full Name

serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit epsilon isoform isoform a

NCBI Official Synonym Full Names

protein phosphatase 2 regulatory subunit B'epsilon

NCBI Official Symbol

PPP2R5E

UniProt Protein Name

Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit epsilon isoform

UniProt Synonym Protein Names

PP2A B subunit isoform B'-epsilon; PP2A B subunit isoform B56-epsilon; PP2A B subunit isoform PR61-epsilon; PP2A B subunit isoform R5-epsilon

Protein Family

Serine/threonine-protein phosphatase

UniProt Gene Name

PPP2R5E

UniProt Entry Name

2A5E_HUMAN

NCBI Protein Information

serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit epsilon isoform

NCBI Summary for PPP2R5E

The protein encoded by this gene belongs to the phosphatase 2A regulatory subunit B family. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The B regulatory subunit might modulate substrate selectivity and catalytic activity. This gene encodes an epsilon isoform of the regulatory subunit B56 subfamily. Multiple transcript variants encoding several different isoforms have been found for this gene. [provided by RefSeq, Aug 2013]

UniProt Comments for PPP2R5E

PPP2R5E: The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment. Belongs to the phosphatase 2A regulatory subunit B56 family.

Protein type: Protein phosphatase, regulatory subunit; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 14q23.1

Cellular Component: cytoplasm; protein phosphatase type 2A complex

Molecular Function: protein binding; protein phosphatase type 2A regulator activity

Biological Process: regulation of protein phosphatase type 2A activity; signal transduction

Research Articles on PPP2R5E

1. PP2A holoenzyme containing B56 is responsible for the dephosphorylation of gamma-H2AX and regulation of DNA repair of double strand breaks induced by camptothecin.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-PPP2R5E antibody

Products by Pathway	Pathway Diagram
AMER1 Mutants Destabilize The Destruction Complex Pathway antibodies	AMER1 Mutants Destabilize The Destruction Complex Pathway Diagram
AMPK Signaling Pathway antibodies	AMPK Signaling Pathway Diagram
AMPK Signaling Pathway antibodies	AMPK Signaling Pathway Diagram
APC Truncation Mutants Have Impaired AXIN Binding Pathway antibodies	APC Truncation Mutants Have Impaired AXIN Binding Pathway Diagram
ARMS-mediated Activation Pathway antibodies	ARMS-mediated Activation Pathway Diagram
AXIN Missense Mutants Destabilize The Destruction Complex Pathway antibodies	AXIN Missense Mutants Destabilize The Destruction Complex Pathway Diagram
AXIN Mutants Destabilize The Destruction Complex, Activating WNT Signaling Pathway antibodies	AXIN Mutants Destabilize The Destruction Complex, Activating WNT Signaling Pathway Diagram
Adaptive Immune System Pathway antibodies	Adaptive Immune System Pathway Diagram
Adrenergic Signaling In Cardiomyocytes Pathway antibodies	Adrenergic Signaling In Cardiomyocytes Pathway Diagram
Adrenergic Signaling In Cardiomyocytes Pathway antibodies	Adrenergic Signaling In Cardiomyocytes Pathway Diagram

Diseases associated with anti-PPP2R5E antibody

Disease Name	Pubmed Publications
Neoplasms, Experimental Antibodies	>2 publications with PPP2R5E and Neoplasms, Experimental
Adenocarcinoma Antibodies	>2 publications with PPP2R5E and Adenocarcinoma
Breast Neoplasms Antibodies	>1 publications with PPP2R5E and Breast Neoplasms
Liver Neoplasms, Experimental Antibodies	>1 publications with PPP2R5E and Liver Neoplasms, Experimental
Carcinoma, Hepatocellular Antibodies	>1 publications with PPP2R5E and Carcinoma, Hepatocellular
Liver Diseases Antibodies	>1 publications with PPP2R5E and Liver Diseases
Precancerous Conditions Antibodies	>1 publications with PPP2R5E and Precancerous Conditions
Liver Neoplasms Antibodies	>1 publications with PPP2R5E and Liver Neoplasms
Inflammation Antibodies	>1 publications with PPP2R5E and Inflammation

Organs/Tissues associated with anti-PPP2R5E antibody

Organ/Tissue Name	Pubmed Publications
Liver Antibodies	>2 publications with PPP2R5E and Liver
Embryonic Tissue Antibodies	>2 publications with PPP2R5E and Embryonic Tissue
Brain Antibodies	>2 publications with PPP2R5E and Brain
Heart Antibodies	>1 publications with PPP2R5E and Heart
Stomach Antibodies	>1 publications with PPP2R5E and Stomach
Lung Antibodies	>1 publications with PPP2R5E and Lung
Intestine Antibodies	>1 publications with PPP2R5E and Intestine
Kidney Antibodies	>1 publications with PPP2R5E and Kidney