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PTBP1 Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS420820

MBS420820 is an antibody targeting PTBP1. Also known as Goat anti-PTBP1/PTB Antibody. Reactive in Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow. Host: Goat. More details

$450 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
PTBP1/PTB, Polyclonal Antibody
Full Product Name
Goat anti-PTBP1/PTB Antibody
Product Gene Name
anti-PTBP1 antibody
Product Synonym Gene Name
PTB
Product Synonym Names
PTBP1; PTB; polypyrimidine tract binding protein 1; PTB2; PTB3; PTB4; pPTB; HNRPI; PTB-1; HNRNPI; RNA binding protein; heterogeneous nuclear ribonucleoprotein polypeptide I; polypyrimidine tract binding protein (heterogeneous nuclear ribonucleoprotein I); PTBP1 antibody; PTB antibody; polypyrimidine tract binding protein 1 antibody; PTB2 antibody; PTB3 antibody; PTB4 antibody; pPTB antibody; HNRPI antibody; PTB-1 antibody; HNRNPI antibody; RNA binding protein antibody; heterogeneous nuclear ribonucleoprotein polypeptide I antibody; polypyrimidine tract binding protein (heterogeneous nuclear ribonucleoprotein I) antibody; HNRNP-I antibody; MGC10830 antibody; MGC8461 antibody; RNA-binding protein antibody; heterogeneous nuclear ribonucleoprotein I antibody; PTBP1 / PTB
Immunogen Sequence
DGIVPDIAVG TKR
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
100ug specific antibody in 200ul (lot specific)
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Antibody/Peptide Pairs
PTBP1 / PTB peptide (MBS426220) is used for blocking the activity of PTBP1/PTB antibody (MBS420820)
Molecular Weight
59,633 Da
OMIM
600693
Immunogen
Peptide with sequence DGIVPDIAVGTKR-C, from the N Terminus of the protein sequence according to NP_002810.1; NP_114367.1; NP_114368.1; NP_787041.1.
Epitope
N Terminus
Note
This antibody is expected to recognise all 4 reported isoforms (represented by NP_002810.1; NP_114367.1; NP_114368.1; NP_787041.1).
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Applications Tested/Suitable for anti-PTBP1 antibody
Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-PTBP1 antibody
Peptide ELISA: Antibody detection limit dilution 1: 32000.
Immunohistochemistry: In paraffin embedded Human Kidney shows nuclear membrane staining in all the convoluted tubules. Recommended concentration, 2-4ug/ml.
Western Blot: Approx 55kDa band observed in Human Liver and Ovary lysates, and 60kDa band observed in nuclear lysates of cell lines HeLa, HepG2, Jurkat and HEK293 (calculated MW of 57.2kDa according to NP_114368.1). Recommended concentration: 0.1-0.3ug/ml.

Western Blot (WB) of anti-PTBP1 antibody

(0.1ug/ml) staining of Human Ovary lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Goat Anti-PTBP1 Polyclonal Antibody – Cat# MBS420820

Western Blot (WB) of anti-PTBP1 antibody

(0. 01ug/ml) staining of HeLa (A), HepG2 (B), Jurkat (C) and HEK293 (D) nuclear lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Goat Anti-PTBP1 Polyclonal Antibody, Western Blot (WB) – Cat# MBS420820

Immunohistochemistry (IHC) of anti-PTBP1 antibody

(2ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.
Goat Anti-PTBP1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS420820

Other Notes

Small volumes of anti-PTBP1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for PTBP1. It may not necessarily be applicable to this product.

NCBI GI #
4506243
NCBI GeneID
5725
NCBI Accession #
NP_002810.1
NCBI Related Accession #
NP_114367.1; NP_114368.1; NP_787041.1
UniProt Secondary Accession #
Q9BUQ0
UniProt Related Accession #
P26599
GenBank Nucleotide #
NM_002819.4
NCBI Official Full Name
polypyrimidine tract-binding protein 1 isoform a
NCBI Official Synonym Full Names
polypyrimidine tract binding protein 1
NCBI Official Symbol
PTBP1
NCBI Official Synonym Symbols
PTB; PTB2; PTB3; PTB4; pPTB; HNRPI; PTB-1; PTB-T; HNRNPI; HNRNP-I
UniProt Protein Name
Polypyrimidine tract-binding protein 1
UniProt Synonym Protein Names
57 kDa RNA-binding protein PPTB-1; Heterogeneous nuclear ribonucleoprotein I; hnRNP I
Protein Family
Polypyrimidine tract-binding protein
UniProt Gene Name
PTBP1
UniProt Synonym Gene Names
PTB; PTB; hnRNP I
UniProt Entry Name
PTBP1_HUMAN
NCBI Protein Information
polypyrimidine tract-binding protein 1

NCBI Summary for PTBP1

This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA-binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has four repeats of quasi-RNA recognition motif (RRM) domains that bind RNAs. This protein binds to the intronic polypyrimidine tracts that requires pre-mRNA splicing and acts via the protein degradation ubiquitin-proteasome pathway. It may also promote the binding of U2 snRNP to pre-mRNAs. This protein is localized in the nucleoplasm and it is also detected in the perinucleolar structure. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008]
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UniProt Comments for PTBP1

hnRNP I: a ubiquitously expressed heterogeneous nuclear ribonucleoprotein (hnRNP). hnRNPs are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. Binds to the polypyrimidine tract of introns. May promote the binding of U2 snRNP to pre-mRNA. Two alternatively spliced isoforms have been described.

Protein type: RNA splicing; RNA-binding

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: membrane; nucleolus; nucleoplasm

Molecular Function: nucleotide binding; poly-pyrimidine tract binding; protein binding; RNA binding

Biological Process: fibroblast growth factor receptor signaling pathway; gene expression; mRNA processing; negative regulation of muscle cell differentiation; negative regulation of nuclear mRNA splicing, via spliceosome; negative regulation of RNA splicing; nuclear mRNA splicing, via spliceosome; regulation of alternative nuclear mRNA splicing, via spliceosome; RNA splicing
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Research Articles on PTBP1

1. Data indicate that polypyrimidine tract-binding protein (PTBP1) is preferentially overexpressed in clinical colorectal cancers

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-PTBP1 antibody

 Products by Pathway  Pathway Diagram
 FGFR2 Alternative Splicing Pathway antibodies  FGFR2 Alternative Splicing Pathway Diagram
 Gene Expression Pathway antibodies  Gene Expression Pathway Diagram
 Processing Of Capped Intron-Containing Pre-mRNA Pathway antibodies  Processing Of Capped Intron-Containing Pre-mRNA Pathway Diagram
 Signal Transduction Pathway antibodies  Signal Transduction Pathway Diagram
 Signaling By FGFR Pathway antibodies  Signaling By FGFR Pathway Diagram
 Signaling By FGFR2 Pathway antibodies  Signaling By FGFR2 Pathway Diagram
 MRNA Splicing Pathway antibodies  MRNA Splicing Pathway Diagram
 MRNA Splicing - Major Pathway antibodies  MRNA Splicing - Major Pathway Diagram
 MRNA Processing Pathway antibodies  MRNA Processing Pathway Diagram

Diseases associated with anti-PTBP1 antibody

 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >12 publications with PTBP1 and Nervous System Diseases
 Disease Models, Animal Antibodies  >4 publications with PTBP1 and Disease Models, Animal
 Carcinoma Antibodies  >4 publications with PTBP1 and Carcinoma
 Cell Transformation, Neoplastic Antibodies  >3 publications with PTBP1 and Cell Transformation, Neoplastic
 Breast Neoplasms Antibodies  >3 publications with PTBP1 and Breast Neoplasms
 Liver Diseases Antibodies  >2 publications with PTBP1 and Liver Diseases
 Lymphoma Antibodies  >2 publications with PTBP1 and Lymphoma
 Liver Cirrhosis Antibodies  >2 publications with PTBP1 and Liver Cirrhosis
 Carcinoma, Hepatocellular Antibodies  >1 publications with PTBP1 and Carcinoma, Hepatocellular
 Neoplasms, Experimental Antibodies  >1 publications with PTBP1 and Neoplasms, Experimental

Organs/Tissues associated with anti-PTBP1 antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >26 publications with PTBP1 and Brain
 Muscle Antibodies  >15 publications with PTBP1 and Muscle
 Nerve Antibodies  >7 publications with PTBP1 and Nerve
 Blood Antibodies  >7 publications with PTBP1 and Blood
 Liver Antibodies  >4 publications with PTBP1 and Liver
 Pancreas Antibodies  >4 publications with PTBP1 and Pancreas
 Skin Antibodies  >4 publications with PTBP1 and Skin
 Heart Antibodies  >4 publications with PTBP1 and Heart
 Embryonic Tissue Antibodies  >4 publications with PTBP1 and Embryonic Tissue
 Lung Antibodies  >3 publications with PTBP1 and Lung
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