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SH2-B/SH2B1 (SH2B1) Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS420824

MBS420824 is an antibody targeting SH2B1. Also known as Goat anti-SH2-B/SH2B1 (isoform2) Antibody. Reactive in Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow. Host: Goat. More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
SH2-B/SH2B1, Polyclonal Antibody
Full Product Name
Goat anti-SH2-B/SH2B1 (isoform2) Antibody
Product Gene Name
Product Synonym Gene Name
SH2-B
Product Synonym Names
SH2B1; SH2B; SH2-B; DKFZP547G1110; SH2-B homolog; SH2-B alpha signaling protein; SH2-B gamma signaling protein; likely ortholog of mouse SH2 domain-containing putative adapter SH2-B; SH2B adaptor protein 1; DKFZp547G1110; FLJ30542; KIAA1299; PSM; SH2 doma; SH2B1 antibody; SH2B antibody; SH2-B antibody; DKFZP547G1110 antibody; SH2-B homolog antibody; SH2-B alpha signaling protein antibody; SH2-B gamma signaling protein antibody; likely ortholog of mouse SH2 domain-containing putative adapter SH2-B antibody; SH2B adaptor protein 1 antibody; DKFZp547G1110 antibody; FLJ30542 antibody; KIAA1299 antibody; PSM antibody; SH2 domain-containing protein 1B antibody; SH2-B signaling protein antibody; pro-rich; PH and SH2 domain-containing signaling mediator antibody; SH2-B / SH2B1 (isoform2)
Immunogen Sequence
PFGASDCVTD HLP
Clonality
Polyclonal
Host
Goat
Species Reactivity
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
100ug specific antibody in 200ul (lot specific)
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Molecular Weight
72,188 Da
OMIM
608937
Immunogen
Peptide with sequence PFGASDCVTDHLP, from the C Terminus of the protein sequence according to NP_056318.2.
Epitope
C Terminus
Note
This antibody is expected to recognise isoform 2 (NP_056318.2) only. Reported variants represent identical protein (NP_001139268.1; NP_056318.2; NP_001139284.1).
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Applications Tested/Suitable for anti-SH2B1 antibody
Peptide ELISA (EIA)
Application Notes for anti-SH2B1 antibody
Peptide ELISA: Antibody detection limit dilution 1: 32000.
Western Blot: Preliminary experiments gave an approx 45-48kDa band in human heart lysate at 1ug/ml. Please note that currently we cannot find an explanation in the literature for the band we observe given the predicted size of 70.6kDa according to NP_056318.1.

Other Notes

Small volumes of anti-SH2B1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for SH2B1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
SH2B adapter protein 1 isoform 2
NCBI Official Synonym Full Names
SH2B adaptor protein 1
NCBI Official Symbol
SH2B1
NCBI Official Synonym Symbols
PSM; SH2B
UniProt Protein Name
SH2B adapter protein 1
UniProt Synonym Protein Names
Pro-rich, PH and SH2 domain-containing signaling mediator; PSM; SH2 domain-containing protein 1B
Protein Family
UniProt Gene Name
SH2B1
UniProt Synonym Gene Names
KIAA1299; SH2B; PSM
UniProt Entry Name
SH2B1_HUMAN
NCBI Protein Information
SH2B adapter protein 1

NCBI Summary for SH2B1

This gene encodes a member of the SH2-domain containing mediators family. The encoded protein mediates activation of various kinases and may function in cytokine and growth factor receptor signaling and cellular transformation. Alternatively spliced transcript variants have been described. [provided by RefSeq, Mar 2009]
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UniProt Comments for SH2B1

SH2-B-beta: an adaptor protein that specifically activates JAK2 and serve as adapter proteins for JAK1, -2 and -3. YXXL motifs in SH2-Bbeta are phosphorylated by JAK2, JAK1, and platelet-derived growth factor receptor and are required for membrane ruffling.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 16p11.2

Cellular Component: cytosol; membrane; nucleus

Molecular Function: protein binding; signal transducer activity

Biological Process: blood coagulation; lamellipodium biogenesis; positive regulation of mitosis; positive regulation of signal transduction
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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